##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2346
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53623188405797	34.0	31.0	34.0	31.0	34.0
2	32.79539641943734	34.0	31.0	34.0	31.0	34.0
3	32.74552429667519	34.0	31.0	34.0	31.0	34.0
4	36.25149190110827	37.0	37.0	37.0	35.0	37.0
5	36.188832054560955	37.0	35.0	37.0	35.0	37.0
6	36.211849957374255	37.0	36.0	37.0	35.0	37.0
7	36.09889173060529	37.0	35.0	37.0	35.0	37.0
8	36.128729752770674	37.0	35.0	37.0	35.0	37.0
9	37.74424552429667	39.0	38.0	39.0	35.0	39.0
10	37.824381926683714	39.0	38.0	39.0	35.0	39.0
11	37.88022165387894	39.0	38.0	39.0	35.0	39.0
12	37.75447570332481	39.0	37.0	39.0	35.0	39.0
13	37.97740835464621	39.0	38.0	39.0	35.0	39.0
14	39.206734867860185	40.0	39.0	41.0	36.0	41.0
15	39.22506393861892	40.0	39.0	41.0	36.0	41.0
16	39.089940323955666	40.0	39.0	41.0	36.0	41.0
17	38.99829497016198	40.0	38.0	41.0	36.0	41.0
18	39.19735720375107	40.0	39.0	41.0	36.0	41.0
19	39.20247229326513	40.0	39.0	41.0	36.0	41.0
20	39.25575447570333	40.0	39.0	41.0	36.0	41.0
21	39.169650468883205	40.0	39.0	41.0	36.0	41.0
22	39.09846547314578	40.0	39.0	41.0	36.0	41.0
23	39.066496163682864	40.0	39.0	41.0	36.0	41.0
24	39.02173913043478	40.0	39.0	41.0	35.0	41.0
25	38.93691389599318	40.0	39.0	41.0	36.0	41.0
26	38.59505541346974	40.0	38.0	41.0	35.0	41.0
27	38.54433077578857	40.0	38.0	41.0	34.0	41.0
28	38.74467178175618	40.0	38.0	41.0	35.0	41.0
29	38.59079283887468	40.0	38.0	41.0	34.0	41.0
30	38.78090366581415	40.0	38.0	41.0	35.0	41.0
31	38.59803921568628	40.0	38.0	41.0	35.0	41.0
32	38.485080988917304	40.0	38.0	41.0	34.0	41.0
33	38.068201193520885	40.0	38.0	41.0	33.0	41.0
34	38.04134697357204	40.0	38.0	41.0	33.0	41.0
35	38.20929241261722	40.0	38.0	41.0	34.0	41.0
36	38.151321398124466	40.0	38.0	41.0	34.0	41.0
37	38.27919863597613	40.0	38.0	41.0	34.0	41.0
38	38.2003410059676	40.0	38.0	41.0	34.0	41.0
39	37.98167092924126	40.0	38.0	41.0	33.0	41.0
40	37.728900255754475	40.0	37.0	41.0	32.0	41.0
41	37.93648763853368	40.0	38.0	41.0	33.0	41.0
42	37.530264279624895	39.0	37.0	41.0	33.0	41.0
43	36.57971014492754	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	2.0
23	2.0
24	5.0
25	2.0
26	3.0
27	8.0
28	12.0
29	11.0
30	25.0
31	31.0
32	46.0
33	32.0
34	72.0
35	88.0
36	153.0
37	288.0
38	656.0
39	909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.044330775788577	19.053708439897697	23.40153452685422	31.50042625745951
2	12.915601023017903	20.28985507246377	33.58908780903666	33.20545609548167
3	13.000852514919012	21.355498721227622	44.032395566922425	21.611253196930946
4	8.780903665814153	16.453537936913897	23.827791986359763	50.93776641091219
5	13.427109974424553	29.923273657289002	32.011935208866156	24.637681159420293
6	32.4381926683717	30.52003410059676	14.663256606990622	22.37851662404092
7	21.824381926683717	34.569479965899404	15.34526854219949	28.26086956521739
8	37.468030690537084	25.404944586530263	17.902813299232736	19.224211423699913
9	17.561807331628305	14.322250639386189	12.489343563512362	55.626598465473144
10	19.01108269394714	21.73913043478261	22.335890878090368	36.91389599317988
11	26.427962489343564	19.352088661551576	18.88320545609548	35.33674339300938
12	14.74850809889173	35.08098891730606	16.624040920716112	33.5464620630861
13	30.477408354646208	13.043478260869565	20.758738277919864	35.72037510656437
14	27.578857630008525	24.765558397271953	16.624040920716112	31.031543052003407
15	26.17220801364024	29.241261722080136	12.745098039215685	31.841432225063937
16	38.53367433930094	22.847399829497018	14.02387041773231	24.595055413469737
17	17.689684569479965	43.7766410912191	14.961636828644501	23.572037510656436
18	25.916453537936913	16.92242114236999	16.92242114236999	40.2387041773231
19	25.95907928388747	24.467178175618074	16.41091219096334	33.16283034953112
20	29.15601023017903	23.52941176470588	17.050298380221655	30.264279624893437
21	23.99829497016198	27.664109121909636	18.88320545609548	29.454390451832907
22	24.722932651321397	25.95907928388747	27.195225916453538	22.122762148337596
23	20.034100596760442	24.765558397271953	15.473145780051151	39.727195225916454
24	25.703324808184142	31.67092924126172	22.975277067348678	19.65046888320546
25	33.71696504688832	22.08013640238704	20.20460358056266	23.99829497016198
26	20.6308610400682	29.198635976129584	30.264279624893437	19.906223358908782
27	25.021312872975276	32.310315430520035	24.85080988917306	17.81756180733163
28	16.879795396419436	19.99147485080989	32.52344416027281	30.605285592497864
29	17.306052855924978	25.2770673486786	24.93606138107417	32.48081841432225
30	24.85080988917306	17.391304347826086	34.78260869565217	22.975277067348678
31	19.99147485080989	23.444160272804773	22.89002557544757	33.67433930093777
32	29.624893435635123	20.716112531969312	32.4381926683717	17.22080136402387
33	16.92242114236999	19.948849104859335	36.91389599317988	26.214833759590796
34	21.526001705029838	34.14322250639386	23.273657289002557	21.057118499573743
35	25.31969309462916	17.007672634271103	40.2387041773231	17.43393009377664
36	27.877237851662407	21.696504688832054	23.742540494458652	26.683716965046887
37	19.47996589940324	21.653878942881498	25.618073316283034	33.248081841432224
38	24.765558397271953	15.77152600170503	27.791986359761296	31.67092924126172
39	29.624893435635123	19.01108269394714	24.722932651321397	26.641091219096335
40	14.74850809889173	14.663256606990622	43.17988064791134	27.408354646206305
41	23.91304347826087	15.814151747655583	24.978687127024724	35.294117647058826
42	19.224211423699913	13.938618925831204	44.37340153452685	22.463768115942027
43	16.92242114236999	13.895993179880648	38.192668371696506	30.988917306052855
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.5
11	1.0
12	1.0
13	2.0
14	3.0
15	8.0
16	13.0
17	13.0
18	13.0
19	13.0
20	12.0
21	11.0
22	10.0
23	9.0
24	9.0
25	9.0
26	9.0
27	9.0
28	9.0
29	11.5
30	14.0
31	18.5
32	23.0
33	23.0
34	28.0
35	33.0
36	29.0
37	25.0
38	42.5
39	60.0
40	60.0
41	72.5
42	85.0
43	94.0
44	103.0
45	106.5
46	110.0
47	110.0
48	136.5
49	163.0
50	244.5
51	326.0
52	251.0
53	176.0
54	176.0
55	351.0
56	526.0
57	444.5
58	363.0
59	225.0
60	87.0
61	87.0
62	77.5
63	68.0
64	52.0
65	36.0
66	30.5
67	25.0
68	25.0
69	27.0
70	29.0
71	22.5
72	16.0
73	10.5
74	5.0
75	5.0
76	3.5
77	2.0
78	1.5
79	1.0
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2346.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.21398124467178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.08900876601484	60.74168797953964
2	2.629804450438301	3.324808184143223
3	0.4045853000674309	0.7672634271099744
4	0.0	0.0
5	0.13486176668914363	0.4262574595055414
6	0.0	0.0
7	0.06743088334457181	0.29838022165387895
8	0.0	0.0
9	0.0	0.0
>10	0.26972353337828725	2.47229326513214
>50	0.20229265003371544	9.718670076726342
>100	0.20229265003371544	22.25063938618926
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT	279	11.892583120204604	TruSeq Adapter, Index 6 (95% over 21bp)
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC	130	5.541346973572037	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT	113	4.816709292412617	No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC	89	3.7936913895993176	RNA PCR Primer, Index 6 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	86	3.6658141517476555	No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG	53	2.2591645353793695	No Hit
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG	23	0.9803921568627451	No Hit
ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT	13	0.5541346973572037	RNA PCR Primer, Index 40 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT	12	0.5115089514066496	No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA	10	0.4262574595055414	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.29838022165387895	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.2131287297527707	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.2131287297527707	No Hit
TCTCCGAGCCCACGAGACCGATCAGTACTCGTATGCCGTCTTC	3	0.1278772378516624	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTA	3	0.1278772378516624	No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATG	3	0.1278772378516624	No Hit
CACCATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC	3	0.1278772378516624	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	3	0.1278772378516624	No Hit
TATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTAT	3	0.1278772378516624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.042625745950554135	0.0
29	0.0	0.0	0.0	0.042625745950554135	0.0
30	0.0	0.0	0.0	0.08525149190110827	0.0
31	0.0	0.0	0.0	0.5115089514066496	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	50	1.2380588E-7	29.599998	37
GCCGTCT	50	1.2380588E-7	29.599998	36
TGCCGTC	65	1.2377768E-6	22.76923	35
TATGCCG	65	1.2377768E-6	22.76923	33
TCTCGTA	65	1.2377768E-6	22.76923	28
ATGCCGT	65	1.2377768E-6	22.76923	34
TCTCCGA	65	1.2377768E-6	22.76923	1
GTATGCC	70	2.3646626E-6	21.142857	32
ATCTCGT	70	2.3646626E-6	21.142857	27
CAGTATC	70	2.3646626E-6	21.142857	23
GTATCTC	70	2.3646626E-6	21.142857	25
AGTATCT	70	2.3646626E-6	21.142857	24
TCAGTAT	70	2.3646626E-6	21.142857	22
TATCTCG	70	2.3646626E-6	21.142857	26
CGTATGC	70	2.3646626E-6	21.142857	31
TCGTATG	70	2.3646626E-6	21.142857	30
CTCGTAT	70	2.3646626E-6	21.142857	29
AGCCCAC	75	4.314201E-6	19.733332	7
GCCCACG	75	4.314201E-6	19.733332	8
CCACGAG	75	4.314201E-6	19.733332	10
>>END_MODULE