FastQCFastQC Report
Fri 10 Feb 2017
ERR1632363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences861097
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.17640289073124166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14010.16269944036502276No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT10850.12600206480802975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5600.026.7589281
CGAACGA551.9019397E-523.54545416
CGGTCGA551.9019397E-523.54545420
TAACGTG400.001930795523.1250025
TAACGCC652.6815487E-622.769234
TCGTTTA502.7013393E-422.230
CGCATCG502.7013393E-422.213
AGTGCGC603.72489E-521.5833328
CATCGTT603.72489E-521.58333228
GTACCGG500.00703348318.51
CGTTTAT609.234253E-418.531
GTATCAA8850.016.7231662
ACACTTA1251.6561717E-716.2799996
CCGATAA803.381332E-416.18759
AATTCCG803.381332E-416.18755
GTGCTAG1504.663889E-916.0333331
CTTACAC1504.663889E-916.0333333
TTAGACT700.002591785515.8571424
CTAGACA1455.3392796E-815.3103454
ATCGCCG855.3622114E-415.23529316
Produced by FastQC (version 0.11.5)