##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632363.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861097
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.50377948128956	34.0	31.0	34.0	31.0	34.0
2	32.667370807237745	34.0	31.0	34.0	31.0	34.0
3	32.682328471705276	34.0	31.0	34.0	31.0	34.0
4	36.2169267806066	37.0	37.0	37.0	35.0	37.0
5	36.079475366886655	37.0	35.0	37.0	35.0	37.0
6	36.1020221879765	37.0	35.0	37.0	35.0	37.0
7	36.09935001515509	37.0	35.0	37.0	35.0	37.0
8	36.07805624685721	37.0	35.0	37.0	35.0	37.0
9	37.807493232469746	39.0	38.0	39.0	35.0	39.0
10	37.71913617165081	39.0	37.0	39.0	35.0	39.0
11	37.82856402937183	39.0	38.0	39.0	35.0	39.0
12	37.78994584814487	39.0	37.0	39.0	35.0	39.0
13	37.82069499719544	39.0	38.0	39.0	35.0	39.0
14	39.15816684995999	40.0	39.0	41.0	36.0	41.0
15	39.17018756307361	40.0	39.0	41.0	36.0	41.0
16	39.057542878444586	40.0	38.0	41.0	36.0	41.0
17	39.10962528031104	40.0	38.0	41.0	36.0	41.0
18	39.119942352603715	40.0	39.0	41.0	36.0	41.0
19	39.17104112544812	40.0	39.0	41.0	36.0	41.0
20	39.15480253676415	40.0	39.0	41.0	36.0	41.0
21	39.12686027241995	40.0	39.0	41.0	36.0	41.0
22	39.103368145516704	40.0	39.0	41.0	36.0	41.0
23	39.05781694745191	40.0	39.0	41.0	36.0	41.0
24	39.006324490736816	40.0	39.0	41.0	36.0	41.0
25	38.949178780090975	40.0	38.0	41.0	35.0	41.0
26	38.84892178233114	40.0	38.0	41.0	35.0	41.0
27	38.783544710990746	40.0	38.0	41.0	35.0	41.0
28	38.72527949812855	40.0	38.0	41.0	35.0	41.0
29	38.58804176532957	40.0	38.0	41.0	35.0	41.0
30	38.54684083210138	40.0	38.0	41.0	35.0	41.0
31	38.46587434400538	40.0	38.0	41.0	34.0	41.0
32	38.392387849452504	40.0	38.0	41.0	34.0	41.0
33	38.25040500663688	40.0	38.0	41.0	34.0	41.0
34	38.252725302724315	40.0	38.0	41.0	34.0	41.0
35	38.219226173125676	40.0	38.0	41.0	34.0	41.0
36	38.19181927239324	40.0	38.0	41.0	34.0	41.0
37	38.15964055152904	40.0	38.0	41.0	34.0	41.0
38	38.089816826675744	40.0	38.0	41.0	34.0	41.0
39	37.907284545178996	40.0	37.0	41.0	33.0	41.0
40	37.91722651455063	40.0	37.0	41.0	33.0	41.0
41	37.912927347325564	40.0	37.0	41.0	33.0	41.0
42	37.788666085237786	40.0	37.0	41.0	33.0	41.0
43	36.95584121184954	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	0.0
14	0.0
15	3.0
16	2.0
17	10.0
18	11.0
19	42.0
20	75.0
21	144.0
22	258.0
23	414.0
24	743.0
25	1146.0
26	1685.0
27	2608.0
28	3664.0
29	5295.0
30	7338.0
31	9924.0
32	13076.0
33	18056.0
34	25969.0
35	37397.0
36	58800.0
37	108108.0
38	248139.0
39	318187.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.561291004381616	18.433114968464643	12.387106214514741	26.618487812638996
2	19.141165281031057	20.061735205209168	34.19266354429292	26.604435969466856
3	20.442180149274705	21.791970010347267	29.03482418357049	28.731025656807535
4	15.354600004412974	15.43113029078025	33.66902915699393	35.545240547812845
5	16.132212747228245	34.73139495318181	33.14295602005349	15.993436279536452
6	35.32366272324721	33.91998810819222	15.43635618286906	15.319992985691508
7	30.3836849971606	29.233640344815974	20.66329345009912	19.71938120792431
8	27.843553049191904	32.779233930672156	19.528461950279702	19.84875106985624
9	27.24187867336665	13.9677643749775	19.27761913001671	39.51273782163914
10	17.700561028548467	26.17602894911955	32.43455731468116	23.688852707650824
11	36.96947033841716	20.929813946628546	20.76374670913962	21.336969005814677
12	22.669803750332424	24.615345309529587	28.475653730067574	24.239197210070408
13	31.56729148980893	19.1067905241802	23.474707262944825	25.851210723066043
14	23.580618675944752	19.788246852561326	24.501536992928788	32.12959747856513
15	26.205758468558134	26.446265635578804	21.513255765610612	25.83472013025246
16	25.841107331694342	25.171960882455753	23.27960729162917	25.707324494220746
17	24.549382938275247	25.51570845096429	24.667139706676483	25.267768904083976
18	24.76515421607554	24.463329915212803	25.50061142937439	25.270904439337265
19	25.44568149697421	24.55646692532897	25.526624758883145	24.471226818813676
20	25.938425055481556	24.209699952502447	25.22061974434936	24.63125524766664
21	26.062220632518752	24.22967447337524	24.63090685486072	25.077198039245292
22	26.050375277117443	24.281468870522136	24.650649113862897	25.01750673849752
23	25.69071777047185	24.23211322301669	24.667488099482405	25.40968090702906
24	25.25615581055328	24.549382938275247	24.64762970954492	25.546831541626553
25	25.64333634886662	24.042471405660454	24.77432855996479	25.539863685508134
26	25.327692466702356	24.453342654776407	24.764573561399004	25.454391317122226
27	25.23920069399847	24.33895368349907	24.788961057813463	25.632884564688997
28	25.1359602925106	24.32919868493329	25.031674712604968	25.50316630995114
29	24.961299365808962	24.551937818852	25.257897774582887	25.228865040756155
30	24.85062658446145	24.730895590159992	25.04746851980671	25.371009305571846
31	25.414558406311947	24.555537877846515	24.708017795904528	25.32188591993701
32	24.654133041922105	24.59328043182127	25.117611604732105	25.634974921524524
33	24.639036020332203	24.49387235119853	25.403758229328403	25.463333399140865
34	25.34197657174511	23.78152519402576	25.285304675315324	25.591193558913805
35	24.811025935521783	24.127711512175747	25.775841746051835	25.28542080625063
36	24.857594440579863	24.124459845987154	25.3652027588065	25.652742954626483
37	24.792096593066752	23.367402278721215	25.911831071296266	25.92867005691577
38	24.266720241738156	23.485391308993066	26.59584228025414	25.652046169014643
39	24.059426522215265	23.20052212468514	27.00230055382843	25.737750799271165
40	23.73646639112667	22.963034361982448	27.728583423238035	25.57191582365285
41	23.378086324769452	22.76781825973148	27.979310112565713	25.87478530293335
42	23.08961708146701	23.009486736105224	28.134693304006404	25.766202878421364
43	22.71521094603744	22.585841084105507	28.45625986387132	26.242688105985735
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	22.0
1	31.0
2	40.0
3	112.0
4	184.0
5	184.0
6	265.0
7	346.0
8	349.5
9	353.0
10	509.5
11	666.0
12	666.0
13	1153.0
14	1640.0
15	2443.0
16	3246.0
17	3214.0
18	3182.0
19	3182.0
20	3946.5
21	4711.0
22	4974.0
23	5237.0
24	6351.5
25	7466.0
26	7466.0
27	9174.0
28	10882.0
29	13084.0
30	15286.0
31	18109.0
32	20932.0
33	20932.0
34	24389.5
35	27847.0
36	31176.5
37	34506.0
38	37648.0
39	40790.0
40	40790.0
41	44094.5
42	47399.0
43	49666.5
44	51934.0
45	54203.0
46	56472.0
47	56472.0
48	58138.0
49	59804.0
50	61174.5
51	62545.0
52	63791.5
53	65038.0
54	65038.0
55	63952.0
56	62866.0
57	60978.5
58	59091.0
59	56284.5
60	53478.0
61	53478.0
62	49699.5
63	45921.0
64	41309.0
65	36697.0
66	32280.0
67	27863.0
68	27863.0
69	23619.0
70	19375.0
71	16673.0
72	13971.0
73	11164.0
74	8357.0
75	8357.0
76	6792.5
77	5228.0
78	4241.5
79	3255.0
80	2620.0
81	1985.0
82	1985.0
83	1587.5
84	1190.0
85	937.5
86	685.0
87	529.0
88	373.0
89	373.0
90	277.5
91	182.0
92	109.5
93	37.0
94	23.5
95	10.0
96	10.0
97	7.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	861097.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.03022589406379
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.91472741018904	68.36237036199844
2	5.952796548278526	9.051849325340392
3	1.5950201735873728	3.6380923231031037
4	0.7224993097585831	2.19727142917001
5	0.4289217887520125	1.6305510244850712
6	0.26489067469739286	1.208381870068425
7	0.19145558049548456	1.0189487723625563
8	0.14547197725786257	0.8848213833737123
9	0.11458273639770018	0.7840576198689398
>10	0.636397972323916	8.805112625140623
>50	0.028795068899723195	1.469448279623279
>100	0.0038282408651430116	0.42343701676532874
>500	1.531296243040903E-4	0.05937664762725685
>1k	4.593888729122709E-4	0.46628132107287
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1519	0.17640289073124166	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1401	0.16269944036502276	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1085	0.12600206480802975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.1613093530693986E-4	0.0
11	0.0	0.0	0.0	1.1613093530693986E-4	0.0
12	0.0	0.0	0.0	1.1613093530693986E-4	0.0
13	0.0	0.0	0.0	1.1613093530693986E-4	0.0
14	0.0	0.0	0.0	1.1613093530693986E-4	0.0
15	0.0	0.0	0.0	2.3226187061387973E-4	0.0
16	0.0	0.0	0.0	4.6452374122775945E-4	0.0
17	0.0	0.0	0.0	8.129165471485791E-4	0.0
18	0.0	0.0	0.0	0.0010451784177624589	0.0
19	0.0	0.0	0.0	0.0013935712236832784	0.0
20	0.0	0.0	0.0	0.0016258330942971581	0.0
21	0.0	0.0	0.0	0.0022064877708318576	0.0
22	0.0	0.0	0.0	0.002787142447366557	0.0
23	0.0	0.0	0.0	0.004064582735742895	0.0
24	0.0	0.0	0.0	0.0065033323771886324	0.0
25	0.0	0.0	0.0	0.00929047482455519	0.0
26	0.0	0.0	0.0	0.012309879142535627	0.0
27	0.0	0.0	0.0	0.02659398418528923	0.0
28	0.0	0.0	0.0	0.07734320291442195	0.0
29	0.0	0.0	0.0	0.15340896554046757	0.0
30	0.0	0.0	0.0	0.2506105583923762	0.0
31	0.0	0.0	0.0	0.52142789952816	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	560	0.0	26.758928	1
CGAACGA	55	1.9019397E-5	23.545454	16
CGGTCGA	55	1.9019397E-5	23.545454	20
TAACGTG	40	0.0019307955	23.125002	5
TAACGCC	65	2.6815487E-6	22.76923	4
TCGTTTA	50	2.7013393E-4	22.2	30
CGCATCG	50	2.7013393E-4	22.2	13
AGTGCGC	60	3.72489E-5	21.583332	8
CATCGTT	60	3.72489E-5	21.583332	28
GTACCGG	50	0.007033483	18.5	1
CGTTTAT	60	9.234253E-4	18.5	31
GTATCAA	885	0.0	16.723166	2
ACACTTA	125	1.6561717E-7	16.279999	6
CCGATAA	80	3.381332E-4	16.1875	9
AATTCCG	80	3.381332E-4	16.1875	5
GTGCTAG	150	4.663889E-9	16.033333	1
CTTACAC	150	4.663889E-9	16.033333	3
TTAGACT	70	0.0025917855	15.857142	4
CTAGACA	145	5.3392796E-8	15.310345	4
ATCGCCG	85	5.3622114E-4	15.235293	16
>>END_MODULE