FastQCFastQC Report
Fri 10 Feb 2017
ERR1632361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33546
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7292.17313539617242No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5391.6067489417516247No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4471.3324986585583973No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT2440.7273594467298635RNA PCR Primer, Index 41 (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2030.605139211828534No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT1620.4829189769272045No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC1110.3308889286353067No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC920.2742502831932272No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.2205926190902045No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC690.20568771239492042RNA PCR Primer, Index 41 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG680.20270673105586362No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT550.16395397364812497No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.12222023490132952No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG360.10731532820604543No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2950.025.7118631
GTATCAA4450.017.0449452
ATGGGAA1451.28026E-512.75862119
TATCAAC6250.012.1363
ATCAACG6250.012.1364
CAACGCA6350.011.9448816
AACGCAG6400.011.85156257
TCTTATA1100.00361680211.77272737
TCAACGC6450.011.7596895
CATGGGA2103.43176E-711.4523818
GTCTCTT2654.5256456E-911.16981137
CAGAGTA6900.010.99275311
AGAGTAC6900.010.99275312
CGCAGAG6950.010.9136699
GCAGAGT7000.010.83571410
GAGTACA4250.010.44705913
ACGCAGA7300.010.390418
AGTACAT4300.010.32558214
AGTACTT3156.1318133E-99.98412712
GAGTACT3156.1318133E-99.98412711
Produced by FastQC (version 0.11.5)