ERR1632360.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632360.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20190
Sequences flagged as poor quality	0
Sequence length	43
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	327	1.6196136701337298	RNA PCR Primer, Index 33 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	245	1.2134720158494305	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	197	0.9757305596830114	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	175	0.866765725606736	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	163	0.8073303615651314	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	132	0.6537890044576523	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.6488360574541853	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	108	0.5349182763744428	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	83	0.41109460128776626	RNA PCR Primer, Index 33 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.39128281327389797	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.27241208519068844	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	51	0.2526002971768202	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	33	0.16344725111441308	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	33	0.16344725111441308	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.15354135710747896	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	26	0.12877662209014362	No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCG	24	0.1188707280832095	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	22	0.10896483407627539	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	21	0.10401188707280833	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	30	7.9138335E-6	37.0	3
TTATACA	30	7.9138335E-6	37.0	2
TACACAT	30	7.9138335E-6	37.0	5
ATACACA	30	7.9138335E-6	37.0	4
CATAGAG	25	0.0053707315	29.599998	31
ACACATC	35	8.5694186E-4	26.42857	6
CTTATAC	45	1.261308E-4	24.666666	1
GGTATCA	145	0.0	21.689655	1
TGCCGTC	70	1.1455591E-4	18.5	37
CACATCT	50	0.0068035815	18.499998	7
GTGGTAT	50	0.0068035815	18.499998	1
TGGGAAG	50	0.0068035815	18.499998	18
GTATCAA	180	0.0	17.472221	2
ATGCCGT	80	3.1810816E-4	16.1875	36
TATGCCG	85	5.0468603E-4	15.235293	35
GTATGCC	95	0.0011724543	13.631579	34
CAACGCA	250	7.2759576E-12	13.32	6
TATCAAC	240	5.820766E-11	13.104167	3
AACGCAG	255	1.0913936E-11	13.058824	7
ATCAACG	255	1.0913936E-11	13.058824	4