##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632360.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20190
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.631104507181774	34.0	31.0	34.0	31.0	34.0
2	32.75998018821198	34.0	31.0	34.0	31.0	34.0
3	32.722634967805845	34.0	31.0	34.0	31.0	34.0
4	36.24447746409113	37.0	37.0	37.0	35.0	37.0
5	36.16711243189698	37.0	35.0	37.0	35.0	37.0
6	36.23912828132739	37.0	36.0	37.0	35.0	37.0
7	36.2345715700842	37.0	36.0	37.0	35.0	37.0
8	36.24081228330857	37.0	36.0	37.0	35.0	37.0
9	37.986379395740464	39.0	38.0	39.0	35.0	39.0
10	37.918870728083206	39.0	38.0	39.0	35.0	39.0
11	38.04878652798415	39.0	38.0	39.0	35.0	39.0
12	37.982565626547796	39.0	38.0	39.0	35.0	39.0
13	38.06453689945518	39.0	38.0	39.0	35.0	39.0
14	39.320752847944526	40.0	39.0	41.0	37.0	41.0
15	39.336255572065376	40.0	39.0	41.0	36.0	41.0
16	39.196334819217434	40.0	39.0	41.0	36.0	41.0
17	39.26805349182764	40.0	39.0	41.0	36.0	41.0
18	39.3579990094106	40.0	39.0	41.0	37.0	41.0
19	39.39450222882615	40.0	39.0	41.0	37.0	41.0
20	39.4098563645369	40.0	39.0	41.0	37.0	41.0
21	39.3949975235265	40.0	39.0	41.0	37.0	41.0
22	39.30138682516097	40.0	39.0	41.0	36.0	41.0
23	39.29217434373452	40.0	39.0	41.0	36.0	41.0
24	39.20807330361565	40.0	39.0	41.0	36.0	41.0
25	39.09569093610698	40.0	39.0	41.0	36.0	41.0
26	38.99088657751362	40.0	38.0	41.0	35.0	41.0
27	38.8691431401684	40.0	38.0	41.0	35.0	41.0
28	38.817979197622584	40.0	38.0	41.0	35.0	41.0
29	38.584546805349184	40.0	38.0	41.0	35.0	41.0
30	38.51624566617137	40.0	38.0	41.0	35.0	41.0
31	38.38954928182268	40.0	38.0	41.0	35.0	41.0
32	38.21045071817731	40.0	37.0	41.0	34.0	41.0
33	37.98167409608717	40.0	37.0	41.0	34.0	41.0
34	37.8877166914314	40.0	37.0	41.0	34.0	41.0
35	37.866864784546806	40.0	37.0	41.0	34.0	41.0
36	37.75052005943537	40.0	36.0	41.0	33.0	41.0
37	37.62526002971768	40.0	36.0	41.0	33.0	41.0
38	37.47538385339277	40.0	36.0	41.0	33.0	41.0
39	37.20926201089648	40.0	35.0	41.0	33.0	41.0
40	37.05527488855869	40.0	35.0	41.0	32.0	41.0
41	36.93046062407132	40.0	35.0	41.0	32.0	41.0
42	36.693164933135215	39.0	35.0	41.0	31.0	41.0
43	35.81287766220901	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	1.0
22	8.0
23	9.0
24	22.0
25	20.0
26	47.0
27	60.0
28	110.0
29	149.0
30	225.0
31	268.0
32	314.0
33	465.0
34	635.0
35	846.0
36	1353.0
37	2949.0
38	4945.0
39	7762.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6691431401684	19.321446260525015	15.002476473501734	24.006934125804854
2	19.41555225359089	21.406636948984644	33.06587419514611	26.11193660227836
3	20.693412580485386	23.179791976225854	32.669638434868745	23.45715700842001
4	14.918276374442794	18.172362555720653	29.658246656760774	37.25111441307578
5	14.972758791480931	35.47795938583457	31.877166914314014	17.67211490837048
6	31.119366022783556	32.42199108469539	16.37444279346211	20.08420009905894
7	25.349182763744427	33.62555720653789	19.10846953937593	21.916790490341754
8	30.237741456166418	30.851906884596335	19.16295195641407	19.74739970282318
9	26.51312530955919	12.610203070827142	19.068845963348192	41.80782565626548
10	21.124318969787023	25.373947498761762	27.40465577018326	26.097077761267958
11	33.590886577513615	21.495789995047055	18.038632986627043	26.874690440812284
12	20.09905894006934	29.73254086181278	25.235264982664685	24.933135215453195
13	33.541357107478944	18.152550767706785	24.55175829618623	23.75433382862803
14	24.140663694898464	23.10054482417038	23.97721644378405	28.7815750371471
15	29.722634967805845	25.354135710747894	20.66864784546805	24.254581475978206
16	26.57751362060426	23.82367508667657	24.987617632491332	24.611193660227837
17	21.154036651807825	30.72808320950966	23.650321941555223	24.46755819712729
18	22.481426448737	23.244180287270925	26.433878157503717	27.84051510648836
19	23.16988608221892	28.44477464091134	27.518573551263003	20.866765725606736
20	24.868746904408123	24.09608717186726	28.286280336800395	22.74888558692422
21	29.024269440316992	22.872709262010897	25.373947498761762	22.729073798910353
22	26.349678058444777	26.46359583952452	26.012877662209018	21.173848439821693
23	27.360079247152058	22.95195641406637	25.83952451708767	23.84843982169391
24	25.017335314512135	26.51312530955919	25.864289252105007	22.605250123823676
25	23.84843982169391	25.59683011391778	25.532441802872707	25.0222882615156
26	25.304606240713223	25.849430411094605	25.62654779593858	23.21941555225359
27	23.041109460128776	27.107478949975235	26.39425458147598	23.45715700842001
28	23.427439326399206	24.85884101040119	27.964338781575037	23.749380881624564
29	22.85289747399703	26.14165428429916	27.077761267954433	23.92768697374938
30	22.20901436354631	25.76523031203566	29.484893511639427	22.540861812778605
31	23.432392273402673	25.269935611688954	26.840019811788014	24.457652303120355
32	22.50123823675087	26.12679544328876	28.860822189202572	22.511144130757803
33	22.1050024764735	25.740465577018323	29.02922238732046	23.125309559187716
34	23.278850916295195	26.2109955423477	27.612679544328877	22.897473997028232
35	21.6394254581476	24.408122833085685	30.425953442298166	23.526498266468547
36	23.031203566121842	26.532937097573058	26.37939574046558	24.056463595839524
37	21.050024764735017	24.87369985141159	28.345715700842	25.73055968301139
38	21.376919266963842	23.417533432392272	29.474987617632493	25.73055968301139
39	22.481426448737	23.55621594848935	30.06438831104507	23.89796929172858
40	20.866765725606736	23.041109460128776	32.5111441307578	23.580980683506684
41	20.79247152055473	23.293709757305596	30.128776622090143	25.785042100049534
42	19.385834571570086	23.055968301139178	33.17979197622585	24.378405151064882
43	19.484893511639427	23.407627538385338	31.570084200099057	25.537394749876174
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	5.5
2	10.0
3	15.0
4	20.0
5	20.0
6	32.5
7	45.0
8	41.5
9	38.0
10	56.0
11	74.0
12	74.0
13	160.5
14	247.0
15	335.0
16	423.0
17	387.0
18	351.0
19	351.0
20	382.5
21	414.0
22	294.0
23	174.0
24	179.0
25	184.0
26	184.0
27	200.0
28	216.0
29	242.5
30	269.0
31	297.0
32	325.0
33	325.0
34	332.5
35	340.0
36	391.5
37	443.0
38	538.5
39	634.0
40	634.0
41	729.5
42	825.0
43	986.0
44	1147.0
45	1311.5
46	1476.0
47	1476.0
48	1700.0
49	1924.0
50	1983.5
51	2043.0
52	1820.5
53	1598.0
54	1598.0
55	1698.0
56	1798.0
57	1688.5
58	1579.0
59	1257.5
60	936.0
61	936.0
62	811.5
63	687.0
64	620.5
65	554.0
66	493.0
67	432.0
68	432.0
69	366.0
70	300.0
71	275.0
72	250.0
73	221.0
74	192.0
75	192.0
76	153.5
77	115.0
78	88.0
79	61.0
80	46.5
81	32.0
82	32.0
83	23.5
84	15.0
85	12.5
86	10.0
87	8.5
88	7.0
89	7.0
90	4.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	20190.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.13670133729569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64532916991934	69.01436354631005
2	5.705178246161853	8.687469044081228
3	1.6588602654176425	3.789004457652303
4	0.6895654436638043	2.1000495294700348
5	0.44236273744470467	1.6840019811788014
6	0.195160031225605	0.8915304606240713
7	0.08456934686442884	0.4507181773155027
8	0.156128024980484	0.950965824665676
9	0.04553734061930783	0.31203566121842496
>10	0.2992453812125943	3.472015849430411
>50	0.026021337496747333	1.3273897969291728
>100	0.052042674993494666	7.32045567112432
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	327	1.6196136701337298	RNA PCR Primer, Index 33 (95% over 22bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	245	1.2134720158494305	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	197	0.9757305596830114	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	175	0.866765725606736	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	163	0.8073303615651314	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	132	0.6537890044576523	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	131	0.6488360574541853	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	108	0.5349182763744428	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	83	0.41109460128776626	RNA PCR Primer, Index 33 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	79	0.39128281327389797	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	55	0.27241208519068844	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	51	0.2526002971768202	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	33	0.16344725111441308	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	33	0.16344725111441308	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.15354135710747896	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	26	0.12877662209014362	No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCG	24	0.1188707280832095	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	22	0.10896483407627539	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	21	0.10401188707280833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.004952947003467063	0.0
20	0.0	0.0	0.0	0.009905894006934125	0.0
21	0.0	0.0	0.0	0.009905894006934125	0.0
22	0.0	0.0	0.0	0.01981178801386825	0.0
23	0.0	0.0	0.0	0.024764735017335313	0.0
24	0.0	0.0	0.0	0.024764735017335313	0.0
25	0.0	0.0	0.0	0.03467062902426944	0.0
26	0.0	0.0	0.0	0.0396235760277365	0.0
27	0.0	0.0	0.0	0.05448241703813769	0.0
28	0.0	0.0	0.0	0.10896483407627539	0.0
29	0.0	0.0	0.0	0.26250619118375434	0.0
30	0.0	0.0	0.0	0.4011887072808321	0.0
31	0.0	0.0	0.0	0.8519068845963348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	30	7.9138335E-6	37.0	3
TTATACA	30	7.9138335E-6	37.0	2
TACACAT	30	7.9138335E-6	37.0	5
ATACACA	30	7.9138335E-6	37.0	4
CATAGAG	25	0.0053707315	29.599998	31
ACACATC	35	8.5694186E-4	26.42857	6
CTTATAC	45	1.261308E-4	24.666666	1
GGTATCA	145	0.0	21.689655	1
TGCCGTC	70	1.1455591E-4	18.5	37
CACATCT	50	0.0068035815	18.499998	7
GTGGTAT	50	0.0068035815	18.499998	1
TGGGAAG	50	0.0068035815	18.499998	18
GTATCAA	180	0.0	17.472221	2
ATGCCGT	80	3.1810816E-4	16.1875	36
TATGCCG	85	5.0468603E-4	15.235293	35
GTATGCC	95	0.0011724543	13.631579	34
CAACGCA	250	7.2759576E-12	13.32	6
TATCAAC	240	5.820766E-11	13.104167	3
AACGCAG	255	1.0913936E-11	13.058824	7
ATCAACG	255	1.0913936E-11	13.058824	4
>>END_MODULE