FastQCFastQC Report
Fri 10 Feb 2017
ERR1632359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54671
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9851.8016864516837081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8431.5419509429130618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5250.9602897331309104No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT5160.9438276234200947TruSeq Adapter, Index 5 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.6859212379506503No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT2950.5395913738545115No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC2240.4097236194691884No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC1830.33472956411991733No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.2926597281922775No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC1510.27619761848146185Illumina Paired End PCR Primer 2 (95% over 23bp)
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG1250.22864041265021673No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1190.21766567284300636No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.16827934371055953No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.15730460390334913No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG780.14267161749373525No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720.13169687768652485No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA640.11706389127691097No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC610.11157652137330579No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG570.10426002816849883No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG560.1024309048672971No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGC550.10060178156609537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC659.0543836E-825.61538535
ATGCCGT659.0543836E-825.61538534
GCCGTCT659.0543836E-825.61538536
CCGTCTT701.854205E-723.78571337
TATGCCG753.6078018E-722.233
TCTTATA1151.2551027E-1020.91304437
GGTATCA6000.019.4251
TCGCCTT953.4810382E-617.52631624
CTCGCCT953.4810382E-617.52631623
GTATGCC1005.674641E-616.65000232
CTCTTAT1452.7739588E-916.58620636
ACCCTGT1151.1900902E-616.08695635
CTCGTAT1059.02192E-615.85714429
GTATCAA7450.015.6442952
GCCTTAT956.865146E-515.57894826
CGCCTTA956.865146E-515.57894825
TTCCTCG1101.4021774E-515.13636420
TCTCGTA1101.4021774E-515.13636428
AGTCGGT1101.4021774E-515.13636411
CGTATGC1101.4021774E-515.13636431
Produced by FastQC (version 0.11.5)