FastQCFastQC Report
Fri 10 Feb 2017
ERR1632358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22891
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4271.865361932637281No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3041.328032851338954No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2611.140186099340352No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1830.7994408282731205TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.4019046786946835No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT770.3363767419509851No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC760.3320082128347385No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC540.2359005722773142RNA PCR Primer, Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC510.22279498492857452No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.1747411646498624No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.14852998995238303No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG340.14852998995238303No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.13542440260364336No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT500.006831109518.516
TCTTATA608.8648574E-418.537
GGTATCA2100.016.7380941
CTCTTAT750.003943088514.836
GTATCAA2500.014.0600012
AGTACTT1501.7549255E-512.33333312
GAGTACT1501.7549255E-512.33333311
AACGCAG3200.012.1406255
CATGGGA1552.4572124E-511.93548418
CAACGCA3300.011.7727274
CGCAGAG3203.6379788E-1211.56257
CAGAGTA3307.2759576E-1211.2121219
AGAGTAC3307.2759576E-1211.21212110
TACTTTT1654.6580015E-511.21212114
GCAGAGT3351.0913936E-1111.0447768
ACGCAGA3551.8189894E-1210.9436626
TCAACGC3551.8189894E-1210.9436623
TATCAAC3551.8189894E-1210.9436621
ATCAACG3551.8189894E-1210.9436622
GAGTACA2205.936399E-710.93181813
Produced by FastQC (version 0.11.5)