FastQCFastQC Report
Fri 10 Feb 2017
ERR1632357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1773215
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.023.1249981
TATCCCG801.6174774E-518.55
CTTATAC10100.017.03465337
GTATAAC803.3838613E-416.18751
GTAATAC1151.2430501E-616.0869563
CGTTTAT1059.348745E-615.85714231
TATATAC957.06313E-515.5789483
GTCTACT957.06313E-515.5789481
TCTTATA16650.015.44444537
CGTTATG855.366191E-415.2352942
GTATCAA9400.015.1542561
TACTAGG1101.45267295E-515.1363642
GTTCTAG3450.015.0144921
CCGTATC1001.0938954E-414.89
GTCTAGA2650.014.66037751
TTAGACT908.277138E-414.3888884
TTACACT2450.014.3469384
ACTGATC2201.8189894E-1114.2954548
TCTAAGC1304.446947E-614.2307693
TCGTTTA1203.302344E-513.87500130
Produced by FastQC (version 0.11.5)