##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1773215
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77073056566745	34.0	31.0	34.0	31.0	34.0
2	32.932907177076665	34.0	31.0	34.0	31.0	34.0
3	32.93449525297271	34.0	33.0	34.0	31.0	34.0
4	36.38179408588355	37.0	37.0	37.0	35.0	37.0
5	36.29460894476981	37.0	37.0	37.0	35.0	37.0
6	36.32844184151386	37.0	37.0	37.0	35.0	37.0
7	36.31892015350648	37.0	37.0	37.0	35.0	37.0
8	36.29893385742846	37.0	37.0	37.0	35.0	37.0
9	38.09275242990839	39.0	39.0	39.0	37.0	39.0
10	38.01283431507178	39.0	38.0	39.0	35.0	39.0
11	38.129288326570666	39.0	38.0	39.0	37.0	39.0
12	38.09598948802035	39.0	38.0	39.0	37.0	39.0
13	38.1202894178089	39.0	39.0	39.0	37.0	39.0
14	39.52472035257992	41.0	39.0	41.0	37.0	41.0
15	39.54545162318162	41.0	39.0	41.0	37.0	41.0
16	39.43624377190583	41.0	39.0	41.0	37.0	41.0
17	39.4807183562061	41.0	39.0	41.0	37.0	41.0
18	39.49752004128095	41.0	39.0	41.0	37.0	41.0
19	39.549159577377814	41.0	39.0	41.0	37.0	41.0
20	39.52024148227936	41.0	39.0	41.0	37.0	41.0
21	39.49333160389462	41.0	39.0	41.0	37.0	41.0
22	39.481903209706665	41.0	39.0	41.0	37.0	41.0
23	39.44135539119622	41.0	39.0	41.0	37.0	41.0
24	39.38794393234887	41.0	39.0	41.0	37.0	41.0
25	39.3270139266812	40.0	39.0	41.0	36.0	41.0
26	39.25006837862301	40.0	39.0	41.0	36.0	41.0
27	39.185933459845536	40.0	39.0	41.0	36.0	41.0
28	39.11861787769673	40.0	39.0	41.0	36.0	41.0
29	38.98793208945334	40.0	39.0	41.0	35.0	41.0
30	38.94842193417042	40.0	39.0	41.0	35.0	41.0
31	38.88355613955442	40.0	38.0	41.0	35.0	41.0
32	38.82156591276298	40.0	38.0	41.0	35.0	41.0
33	38.688407215143116	40.0	38.0	41.0	35.0	41.0
34	38.69561333510037	40.0	38.0	41.0	35.0	41.0
35	38.673689315734414	40.0	38.0	41.0	35.0	41.0
36	38.64510394960566	40.0	38.0	41.0	35.0	41.0
37	38.61796228883694	40.0	38.0	41.0	35.0	41.0
38	38.54937951686626	40.0	38.0	41.0	35.0	41.0
39	38.35836376299547	40.0	38.0	41.0	34.0	41.0
40	38.37612133892393	40.0	38.0	41.0	34.0	41.0
41	38.38200669405571	40.0	38.0	41.0	34.0	41.0
42	38.26144150596515	40.0	38.0	41.0	34.0	41.0
43	37.39493236860731	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	1.0
13	0.0
14	1.0
15	0.0
16	2.0
17	14.0
18	30.0
19	41.0
20	92.0
21	180.0
22	365.0
23	638.0
24	1060.0
25	1617.0
26	2456.0
27	3526.0
28	5231.0
29	7259.0
30	10024.0
31	14006.0
32	19674.0
33	27498.0
34	41654.0
35	63221.0
36	101833.0
37	187158.0
38	444515.0
39	841116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.601255346926344	17.005100904289666	11.542988300911057	27.85065544787293
2	20.18294453859233	19.401200644027938	32.50513897073959	27.910715846640144
3	20.84874084642866	20.67431191367093	28.283033924256223	30.19391331564418
4	15.8844810133007	14.724610382835696	31.950214722974934	37.440693880888674
5	16.841612551213476	34.13906379091086	32.72051048519215	16.298813172683516
6	37.686518555279534	32.12120357655445	14.587571163113328	15.604706705052687
7	31.97948359336008	27.865656448879577	19.732801718911695	20.422058238848646
8	28.99586344577505	31.360776893946866	18.884906793592428	20.758452866685655
9	27.812363418987545	13.197891964595382	17.8991267274414	41.09061788897567
10	18.10744889931565	25.33843893718472	31.606827147300244	24.947285016199388
11	38.71747080867238	19.76274732618436	19.483142202158227	22.03663966298503
12	23.73367019791734	23.241456901729347	27.062820921320878	25.962051979032434
13	32.78750743705642	17.750752164853107	22.432643531664237	27.02909686642624
14	24.794173295398473	18.527928085426755	22.369368632681315	34.30852998649346
15	27.58503621952217	25.523921239105242	19.893695913919068	26.997346627453524
16	27.90535834628062	23.383346069145592	21.52169928632456	27.189596298249224
17	26.156444649971945	24.33619160677075	22.831015979449756	26.676347763807545
18	26.350498952467692	23.065843679418457	23.808336834506814	26.775320533607033
19	27.234317327566032	23.107181024297674	23.282963430830442	26.375538217305856
20	27.221628510925072	23.178633160671435	23.41035914990568	26.189379178497813
21	27.45662539511565	23.163237396480405	22.862991797384975	26.517145411018966
22	27.749765256892147	22.758548737744718	22.39322360796632	27.098462397396816
23	26.690446448964167	23.019938360548494	22.93715088130881	27.352464309178526
24	26.961028414490062	23.08107025938761	22.654162072845086	27.303739253277236
25	27.43598492004636	22.66921946859236	22.877710824688492	27.017084786672797
26	27.061523842286466	22.837896137806187	22.797348319295743	27.3032317006116
27	27.172847060283157	22.746367473769396	22.844832690903246	27.235952775044197
28	26.390539218312497	23.060542573799566	23.214049057784873	27.334869150103064
29	26.60060962714617	23.17440355512445	23.17231694972127	27.05266986800811
30	26.229814207527006	23.560199975750262	23.403197017846118	26.806788798876617
31	27.17329821820817	23.156526422345852	22.831861900559154	26.838313458886827
32	26.2268252862738	22.937714828715077	23.467092258975928	27.368367626035194
33	26.1027004621549	22.91944293275209	23.43427051993131	27.5435860851617
34	26.769737454285014	22.284720127000956	23.54818789599682	27.397354522717208
35	26.26962889440931	22.679032153461367	23.97560363520498	27.075735316924344
36	25.97434603248901	22.644349387976074	24.163962068897455	27.21734251063746
37	26.480714408574258	21.745981169796107	24.467816931392978	27.30548749023666
38	25.577834611144162	22.00917542429993	25.41028583674286	27.002704127813036
39	25.490084394729344	21.833956965173428	25.827268548935127	26.848690091162098
40	25.19034634829956	21.626085951224187	26.171107282534834	27.012460417941426
41	24.532388909410308	21.401183725605748	27.249769486497687	26.81665787848625
42	24.38074345186568	21.276438559339955	27.088931686230943	27.253886302563423
43	23.39930578074289	21.03907309604306	27.714067386075573	27.847553737138476
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	17.5
2	22.0
3	58.5
4	95.0
5	95.0
6	118.5
7	142.0
8	148.0
9	154.0
10	228.5
11	303.0
12	303.0
13	537.0
14	771.0
15	1218.5
16	1666.0
17	1803.5
18	1941.0
19	1941.0
20	2487.5
21	3034.0
22	3637.0
23	4240.0
24	5357.0
25	6474.0
26	6474.0
27	8220.5
28	9967.0
29	13547.5
30	17128.0
31	20948.0
32	24768.0
33	24768.0
34	30441.0
35	36114.0
36	42288.0
37	48462.0
38	55971.0
39	63480.0
40	63480.0
41	72475.5
42	81471.0
43	86758.0
44	92045.0
45	102538.0
46	113031.0
47	113031.0
48	119944.5
49	126858.0
50	134749.0
51	142640.0
52	149861.5
53	157083.0
54	157083.0
55	154043.0
56	151003.0
57	145335.5
58	139668.0
59	136999.5
60	134331.0
61	134331.0
62	125615.0
63	116899.0
64	103303.5
65	89708.0
66	80312.0
67	70916.0
68	70916.0
69	60648.5
70	50381.0
71	43020.5
72	35660.0
73	28422.0
74	21184.0
75	21184.0
76	16952.0
77	12720.0
78	10512.5
79	8305.0
80	6630.5
81	4956.0
82	4956.0
83	3812.0
84	2668.0
85	2127.0
86	1586.0
87	1170.5
88	755.0
89	755.0
90	600.0
91	445.0
92	262.0
93	79.0
94	55.5
95	32.0
96	32.0
97	22.0
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1773215.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.09025043858202
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.81685575094575	53.283900654278895
2	7.836879765523716	9.73187654596847
3	2.371716440273991	4.417814032377431
4	1.1227953467132434	2.788585770747993
5	0.6468687971673269	2.0082122808511826
6	0.40928439150814333	1.5247542221605963
7	0.2832356120879891	1.2310319061367818
8	0.20488385053059774	1.0177031672212657
9	0.1536497674429183	0.8586137286324614
>10	0.9866438074761079	11.823782547772803
>50	0.10826125047495368	4.69412418791987
>100	0.05788323969004408	6.218245476446018
>500	0.001041980165260609	0.4013554794861495
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1278948125297835E-4	0.0
10	0.0	0.0	0.0	1.6918422187946753E-4	0.0
11	0.0	0.0	0.0	1.6918422187946753E-4	0.0
12	0.0	0.0	0.0	3.3836844375893506E-4	0.0
13	0.0	0.0	0.0	3.3836844375893506E-4	0.0
14	0.0	0.0	0.0	3.3836844375893506E-4	0.0
15	0.0	0.0	0.0	5.075526656384026E-4	0.0
16	0.0	0.0	0.0	8.459211093973376E-4	0.0
17	0.0	0.0	0.0	0.0011842895531562726	0.0
18	0.0	0.0	0.0	0.0012406842937827617	0.0
19	0.0	0.0	0.0	0.0014662632562887185	0.0
20	0.0	0.0	0.0	0.0015790527375416968	0.0
21	0.0	0.0	0.0	0.0021430001438065885	0.0
22	0.0	0.0	0.0	0.002932526512577437	0.0
23	0.0	0.0	0.0	0.003947631843854242	0.0
24	0.0	0.0	0.0	0.006034237247034341	0.0
25	0.0	0.0	0.0	0.007218526800190615	0.0
26	0.0	0.0	0.0	0.010320237534647518	0.0
27	0.0	0.0	0.0	0.023685791063125454	0.0
28	0.0	0.0	0.0	0.07258003118629157	0.0
29	0.0	0.0	0.0	0.14826177310704003	0.0
30	0.0	0.0	0.0	0.2411439109188677	0.0
31	0.0	0.0	0.0	0.5117258764447628	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	600	0.0	23.124998	1
TATCCCG	80	1.6174774E-5	18.5	5
CTTATAC	1010	0.0	17.034653	37
GTATAAC	80	3.3838613E-4	16.1875	1
GTAATAC	115	1.2430501E-6	16.086956	3
CGTTTAT	105	9.348745E-6	15.857142	31
TATATAC	95	7.06313E-5	15.578948	3
GTCTACT	95	7.06313E-5	15.578948	1
TCTTATA	1665	0.0	15.444445	37
CGTTATG	85	5.366191E-4	15.235294	2
GTATCAA	940	0.0	15.154256	1
TACTAGG	110	1.45267295E-5	15.136364	2
GTTCTAG	345	0.0	15.014492	1
CCGTATC	100	1.0938954E-4	14.8	9
GTCTAGA	265	0.0	14.6603775	1
TTAGACT	90	8.277138E-4	14.388888	4
TTACACT	245	0.0	14.346938	4
ACTGATC	220	1.8189894E-11	14.295454	8
TCTAAGC	130	4.446947E-6	14.230769	3
TCGTTTA	120	3.302344E-5	13.875001	30
>>END_MODULE