FastQCFastQC Report
Fri 10 Feb 2017
ERR1632356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28509
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT610.21396751902907854RNA PCR Primer, Index 47 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTTAT400.001885611623.12537
TCTCTTA500.00687198718.49999836
CTGTCTC1552.508534E-511.93548437
Produced by FastQC (version 0.11.5)