##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28509
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.702795608404365	31.0	31.0	34.0	30.0	34.0
2	31.879792346276613	31.0	31.0	34.0	30.0	34.0
3	31.91108071135431	33.0	31.0	34.0	30.0	34.0
4	35.695183976989725	37.0	35.0	37.0	33.0	37.0
5	35.15886211371848	37.0	35.0	37.0	32.0	37.0
6	35.12413623767933	37.0	35.0	37.0	32.0	37.0
7	35.15093479252166	37.0	35.0	37.0	32.0	37.0
8	35.058998912624084	37.0	35.0	37.0	32.0	37.0
9	36.689466484268124	38.0	35.0	39.0	32.0	39.0
10	36.4546283629731	38.0	35.0	39.0	32.0	39.0
11	36.634676768739695	38.0	35.0	39.0	32.0	39.0
12	36.551474972815605	38.0	35.0	39.0	32.0	39.0
13	36.634361078957525	38.0	35.0	39.0	32.0	39.0
14	37.69108000982146	39.0	37.0	40.0	33.0	41.0
15	37.67319092216493	39.0	37.0	40.0	33.0	41.0
16	37.50854116243993	39.0	37.0	40.0	32.0	41.0
17	37.555438633414006	39.0	37.0	40.0	32.0	41.0
18	37.58570977586025	39.0	37.0	40.0	33.0	41.0
19	37.61145603142867	39.0	37.0	40.0	33.0	41.0
20	37.60598407520432	39.0	37.0	40.0	32.0	41.0
21	37.521800133291244	39.0	37.0	40.0	32.0	41.0
22	37.44003647970816	39.0	36.0	40.0	32.0	41.0
23	37.38012557438002	39.0	36.0	40.0	32.0	41.0
24	37.2677049352836	39.0	36.0	40.0	32.0	41.0
25	37.21814163948227	39.0	36.0	40.0	32.0	41.0
26	37.04531902206321	39.0	36.0	40.0	31.0	41.0
27	36.93479252165983	39.0	36.0	40.0	31.0	41.0
28	36.82500263074819	39.0	36.0	40.0	31.0	41.0
29	36.626679294257954	38.0	36.0	40.0	30.0	41.0
30	36.57560770283069	38.0	35.0	40.0	30.0	41.0
31	36.45803079729208	38.0	35.0	40.0	30.0	41.0
32	36.358027289627834	38.0	35.0	40.0	30.0	41.0
33	36.167105124697464	38.0	35.0	40.0	29.0	41.0
34	36.15602090567891	38.0	35.0	40.0	29.0	41.0
35	36.069416675435825	38.0	35.0	40.0	29.0	41.0
36	36.051106668069735	38.0	35.0	40.0	29.0	41.0
37	36.039391069486825	38.0	35.0	40.0	30.0	41.0
38	35.886035988635165	38.0	34.0	40.0	29.0	41.0
39	35.71170507559017	38.0	34.0	40.0	28.0	41.0
40	35.69697288575538	38.0	34.0	40.0	28.0	41.0
41	35.66151040022449	38.0	34.0	40.0	28.0	41.0
42	35.54144305307096	38.0	34.0	40.0	28.0	41.0
43	34.525904100459506	37.0	33.0	39.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	0.0
15	1.0
16	1.0
17	1.0
18	5.0
19	4.0
20	12.0
21	13.0
22	31.0
23	42.0
24	80.0
25	121.0
26	142.0
27	237.0
28	339.0
29	428.0
30	603.0
31	824.0
32	1018.0
33	1395.0
34	2025.0
35	2680.0
36	3913.0
37	5328.0
38	6384.0
39	2880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.38629906345365	17.50675225367428	11.880458802483426	27.226489880388648
2	21.70191869234277	19.355291311515664	30.183450840085584	28.75933915605598
3	22.087761759444387	20.568943140762567	27.489564698867024	29.853730400926022
4	17.362938019572766	14.812866112455717	30.148374197621802	37.67582167034971
5	17.443614297239467	34.16114209547862	31.165596829071525	17.229646778210387
6	38.80879722192992	31.256796099477356	14.160440562629345	15.77396611596338
7	32.57217019186923	27.636886597214914	18.65726612648637	21.13367708442948
8	29.394226384650462	30.856922375390226	18.173208460486162	21.57564277947315
9	28.243712511838364	12.648637272440283	17.089340208355257	42.0183100073661
10	19.029078536602476	24.71500227998176	30.13083587638991	26.125083307025854
11	39.73832824722018	19.520151531095443	18.260900066645622	22.48062015503876
12	24.318636220141006	23.10849205514048	25.914623452243152	26.65824827247536
13	34.680276403942614	16.97358728822477	20.807464309516295	27.53867199831632
14	25.40951980076467	18.025886562138272	21.161738398400505	35.40285523869655
15	28.447157038128314	24.743063593952787	19.092216493037288	27.71756287488162
16	28.59097127222982	23.276859938966645	20.295345329545057	27.83682345925848
17	27.261566522852434	23.234767968010104	21.919393875618226	27.584271633519243
18	27.240520537374163	22.20702234382125	22.41397453435757	28.138482584447015
19	27.808762145287453	22.66652636009681	22.161422708618332	27.3632887859974
20	28.21565119786734	21.705426356589147	22.764740958995404	27.314181486548105
21	28.42961871689642	22.256129643270548	21.473920516328178	27.840331123504857
22	29.18376652986776	22.0526851169806	21.649303728647094	27.11424462450454
23	27.871900101722265	22.042162124241468	21.747518327545688	28.338419446490583
24	27.777193167070045	22.603388403662	21.729980006313795	27.889438422954154
25	27.878915430215017	22.20702234382125	21.729980006313795	28.184082219649937
26	28.103405941983233	22.33329825669087	21.649303728647094	27.913992072678802
27	28.06832929951945	22.115823073415413	21.435336209618015	28.380511417447124
28	28.008699007331018	22.035146795748712	22.1017924164299	27.854361780490372
29	27.47553404188151	22.340313585183626	22.568311761198217	27.615840611736648
30	26.89326177698271	23.318951909923182	22.112315409169035	27.675470903925074
31	28.026237328562907	22.424497527096708	21.516012487284716	28.03325265705567
32	27.047599003823354	22.50517380476341	22.0526851169806	28.394542074432632
33	26.931846083692868	21.779087305763092	22.771756287488163	28.517310323055877
34	27.21596688764951	21.78259497000947	22.431512855589464	28.569925286751552
35	27.026553018345084	22.33329825669087	23.336490231155075	27.303658493808975
36	26.689817250692766	22.0526851169806	23.259321617734752	27.998176014591884
37	27.05812199656249	21.326598617980284	23.539934757445018	28.075344628012207
38	25.725209582938717	21.50899715879196	25.125398996808023	27.640394261461292
39	26.135606299764984	21.228384019081695	24.725525272720894	27.910484408432424
40	25.044722719141323	21.06703146374829	26.363604475779578	27.524641341330806
41	24.8868778280543	21.217861026342558	26.31449717633028	27.58076396927286
42	24.8062015503876	20.505805184327755	27.293135501069838	27.39485776421481
43	23.985408116735066	20.51983584131327	27.016030025605946	28.478726016345718
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.5
9	2.0
10	3.0
11	4.0
12	4.0
13	7.5
14	11.0
15	18.0
16	25.0
17	25.5
18	26.0
19	26.0
20	30.5
21	35.0
22	39.0
23	43.0
24	54.5
25	66.0
26	66.0
27	92.0
28	118.0
29	154.0
30	190.0
31	216.0
32	242.0
33	242.0
34	337.5
35	433.0
36	524.0
37	615.0
38	710.0
39	805.0
40	805.0
41	949.0
42	1093.0
43	1206.5
44	1320.0
45	1419.5
46	1519.0
47	1519.0
48	1742.0
49	1965.0
50	2092.5
51	2220.0
52	2428.0
53	2636.0
54	2636.0
55	2570.0
56	2504.0
57	2497.0
58	2490.0
59	2458.0
60	2426.0
61	2426.0
62	2256.5
63	2087.0
64	1884.0
65	1681.0
66	1480.5
67	1280.0
68	1280.0
69	1093.0
70	906.0
71	774.0
72	642.0
73	551.0
74	460.0
75	460.0
76	365.5
77	271.0
78	229.0
79	187.0
80	148.5
81	110.0
82	110.0
83	79.5
84	49.0
85	38.5
86	28.0
87	18.5
88	9.0
89	9.0
90	7.0
91	5.0
92	4.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	28509.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	92.81279595917078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.80120937263794	88.91578098144446
2	2.8382464096749813	5.268511698060261
3	0.6575963718820862	1.8310007366094918
4	0.30990173847316704	1.1505138728120943
5	0.15117157974300832	0.7015328492756674
6	0.08314436885865457	0.4630116805219404
7	0.04157218442932729	0.27009014697113193
8	0.030234315948601664	0.22449051176821355
9	0.015117157974300832	0.12627591286962012
>10	0.06802721088435373	0.8348240906380441
>50	0.003779289493575208	0.21396751902907854
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	61	0.21396751902907854	RNA PCR Primer, Index 47 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0035076642463783368	0.0
18	0.0	0.0	0.0	0.0035076642463783368	0.0
19	0.0	0.0	0.0	0.0035076642463783368	0.0
20	0.0	0.0	0.0	0.0035076642463783368	0.0
21	0.0	0.0	0.0	0.0035076642463783368	0.0
22	0.0	0.0	0.0	0.0035076642463783368	0.0
23	0.0	0.0	0.0	0.0035076642463783368	0.0
24	0.0	0.0	0.0	0.0035076642463783368	0.0
25	0.0	0.0	0.0	0.0035076642463783368	0.0
26	0.0	0.0	0.0	0.0070153284927566735	0.0
27	0.0	0.0	0.0	0.02104598547827002	0.0
28	0.0	0.0	0.0	0.0947069346522151	0.0
29	0.0	0.0	0.0	0.16135255533340348	0.0
30	0.0	0.0	0.0	0.22799817601459188	0.0
31	0.0	0.0	0.0	0.4454733592900488	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTTAT	40	0.0018856116	23.125	37
TCTCTTA	50	0.006871987	18.499998	36
CTGTCTC	155	2.508534E-5	11.935484	37
>>END_MODULE