##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141815
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.637125832951384	34.0	31.0	34.0	31.0	34.0
2	32.80521806578994	34.0	31.0	34.0	31.0	34.0
3	32.80901879208828	34.0	31.0	34.0	31.0	34.0
4	36.30372668617565	37.0	37.0	37.0	35.0	37.0
5	36.185995839650246	37.0	35.0	37.0	35.0	37.0
6	36.223354370130096	37.0	36.0	37.0	35.0	37.0
7	36.218954271409935	37.0	36.0	37.0	35.0	37.0
8	36.20205196911469	37.0	36.0	37.0	35.0	37.0
9	37.97762578006558	39.0	38.0	39.0	35.0	39.0
10	37.891809752141874	39.0	38.0	39.0	35.0	39.0
11	38.002813524662415	39.0	38.0	39.0	35.0	39.0
12	37.958029827592284	39.0	38.0	39.0	35.0	39.0
13	37.98646828614744	39.0	38.0	39.0	35.0	39.0
14	39.372062193703066	40.0	39.0	41.0	37.0	41.0
15	39.38981772026936	40.0	39.0	41.0	37.0	41.0
16	39.287078235729645	40.0	39.0	41.0	36.0	41.0
17	39.32057257694884	40.0	39.0	41.0	36.0	41.0
18	39.31553079716532	40.0	39.0	41.0	36.0	41.0
19	39.3745301977929	40.0	39.0	41.0	37.0	41.0
20	39.34161407467475	40.0	39.0	41.0	36.0	41.0
21	39.31498783626556	40.0	39.0	41.0	36.0	41.0
22	39.305764552409826	40.0	39.0	41.0	36.0	41.0
23	39.26798998695484	40.0	39.0	41.0	36.0	41.0
24	39.206649508162045	40.0	39.0	41.0	36.0	41.0
25	39.13942107675493	40.0	39.0	41.0	36.0	41.0
26	39.06708035116173	40.0	39.0	41.0	36.0	41.0
27	38.984881712089695	40.0	39.0	41.0	36.0	41.0
28	38.910376194337694	40.0	38.0	41.0	35.0	41.0
29	38.79769417903607	40.0	38.0	41.0	35.0	41.0
30	38.747558438811126	40.0	38.0	41.0	35.0	41.0
31	38.69008920071925	40.0	38.0	41.0	35.0	41.0
32	38.61932094630328	40.0	38.0	41.0	35.0	41.0
33	38.49573035292458	40.0	38.0	41.0	34.0	41.0
34	38.47899023375525	40.0	38.0	41.0	34.0	41.0
35	38.45506469696436	40.0	38.0	41.0	34.0	41.0
36	38.415174699432356	40.0	38.0	41.0	34.0	41.0
37	38.39358318936643	40.0	38.0	41.0	34.0	41.0
38	38.3246483094172	40.0	38.0	41.0	34.0	41.0
39	38.161802348129605	40.0	38.0	41.0	34.0	41.0
40	38.16996086450658	40.0	38.0	41.0	34.0	41.0
41	38.16485562176074	40.0	38.0	41.0	34.0	41.0
42	38.067989986954835	40.0	37.0	41.0	34.0	41.0
43	37.20271480449882	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	2.0
20	19.0
21	19.0
22	31.0
23	85.0
24	88.0
25	180.0
26	239.0
27	325.0
28	475.0
29	675.0
30	970.0
31	1353.0
32	1781.0
33	2489.0
34	3856.0
35	5567.0
36	9005.0
37	16266.0
38	38256.0
39	60131.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10333885696153	17.263336036385432	11.855586503543348	27.777738603109686
2	20.499947114198076	19.868138067200224	32.019885061523816	27.612029757077885
3	21.050664598244193	20.98931706801114	28.721221309452456	29.23879702429221
4	16.075873497161794	15.0188626026866	31.89366428092938	37.011599619222224
5	16.82191587631774	34.27070479145365	32.62419349152064	16.283185840707965
6	37.157564432535345	32.53675563233791	14.818601699397101	15.487078235729648
7	31.5121813630434	28.39121390544019	19.640376546909707	20.456228184606708
8	28.747311638402145	31.402178895039313	19.06215844586257	20.788351020695977
9	27.746712265980328	13.253181962415825	17.754116278249832	41.24598949335402
10	18.18919014208652	25.540316609667524	31.49243733032472	24.778055917921236
11	38.64753375877023	19.479603709057574	19.686916052603745	22.185946479568454
12	23.573669922081585	23.499629799386526	26.79970383950922	26.12699643902267
13	32.80753093819413	17.780911751225187	22.57730141381377	26.834255896766912
14	24.869724641257974	18.780805979621338	22.157035574516097	34.19243380460459
15	27.654338398617917	25.719423192187	19.674928604167402	26.95130980502768
16	27.638120086027573	23.90508761414519	21.23259175686634	27.2242005429609
17	26.657264746324437	24.228043577900788	22.43345203257765	26.681239643197124
18	26.16577936043437	22.99968268518845	23.87970242922117	26.954835525156014
19	27.52600218594648	22.954553467545747	23.39315305151077	26.126291294997
20	27.6331840778479	22.511017875401052	23.34238268166273	26.51341536508832
21	27.57959313189719	23.003913549342453	22.420759440115642	26.995733878644714
22	27.688185311849946	22.620315199379473	22.43345203257765	27.25804745619293
23	27.02464478369707	22.936924866904064	22.434862320628987	27.603568028769875
24	27.151570708317173	23.16398124316892	22.606212318866127	27.07823572964778
25	27.56407996333251	22.346014173394916	22.618904911328137	27.471000951944436
26	27.277791488911614	23.092056552550858	22.368578782216268	27.261573176321264
27	27.268624616577934	22.51454359552939	22.901667665620703	27.315164122271973
28	26.928040052180656	22.71268906674188	23.054683919190495	27.30458696188697
29	26.255332651694108	23.318407784790043	23.211225892888624	27.215033670627225
30	26.162958784331703	23.89662588583718	22.95173289144308	26.98868243838804
31	27.061312273031767	23.36283185840708	22.75358742023058	26.822268448330572
32	26.080456933328634	23.139301202270563	23.446038853435812	27.33420301096499
33	26.203857137820403	22.798716637873287	23.43687198110214	27.560554243204173
34	26.661495610478443	22.600571166660792	23.637132884391637	27.100800338469135
35	26.468991291471283	22.782498325282937	23.826111483270456	26.92239889997532
36	25.80756619539541	22.944681451186405	24.039064979021965	27.208687374396224
37	26.40059232098156	22.003314176920636	24.33452032577654	27.261573176321264
38	25.459225046715794	22.188061911645455	25.313260233402673	27.03945280823608
39	25.204668053449918	21.921517469943236	25.85551598914078	27.018298487466062
40	24.574974438529072	21.56541973698128	26.810280999894225	27.049324824595423
41	23.931883087120546	21.543560272185594	27.463949511687762	27.060607129006097
42	24.138490286641044	21.13810245742693	27.31657441032331	27.406832845608715
43	23.089941120473856	20.755914395515283	28.395444769594192	27.758699714416668
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	7.5
4	13.0
5	13.0
6	11.5
7	10.0
8	8.5
9	7.0
10	10.0
11	13.0
12	13.0
13	26.0
14	39.0
15	66.5
16	94.0
17	95.0
18	96.0
19	96.0
20	143.0
21	190.0
22	236.5
23	283.0
24	392.5
25	502.0
26	502.0
27	634.0
28	766.0
29	1077.0
30	1388.0
31	1683.0
32	1978.0
33	1978.0
34	2414.0
35	2850.0
36	3345.5
37	3841.0
38	4635.5
39	5430.0
40	5430.0
41	6142.5
42	6855.0
43	7128.0
44	7401.0
45	8155.5
46	8910.0
47	8910.0
48	9577.0
49	10244.0
50	10933.5
51	11623.0
52	12517.5
53	13412.0
54	13412.0
55	12756.0
56	12100.0
57	11555.5
58	11011.0
59	10422.0
60	9833.0
61	9833.0
62	9345.5
63	8858.0
64	8029.0
65	7200.0
66	6336.0
67	5472.0
68	5472.0
69	4697.5
70	3923.0
71	3421.0
72	2919.0
73	2362.5
74	1806.0
75	1806.0
76	1463.0
77	1120.0
78	950.0
79	780.0
80	596.5
81	413.0
82	413.0
83	309.0
84	205.0
85	164.0
86	123.0
87	92.0
88	61.0
89	61.0
90	47.0
91	33.0
92	18.5
93	4.0
94	3.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141815.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.3270856650968
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.40076764465562	69.76354221869238
2	4.68570186239455	7.15291934904185
3	1.3238118184899978	3.0312809422306097
4	0.6717255892232221	2.0508342636831403
5	0.38879967010990274	1.4837972863519961
6	0.26486057699457094	1.212962156974295
7	0.19589665157756422	1.0466554354526454
8	0.14992088859430006	0.915441960537966
9	0.11993670935271618	0.8238977540239125
>10	0.7655959935843846	10.680156054994521
>50	0.029984177293788543	1.5638799937486398
>100	0.002998417729378855	0.27463258426805387
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	7.051440256672425E-4	0.0
23	0.0	0.0	0.0	7.051440256672425E-4	0.0
24	0.0	0.0	0.0	0.001410288051334485	0.0
25	0.0	0.0	0.0	0.0021154320770017275	0.0
26	0.0	0.0	0.0	0.007756584282339668	0.0
27	0.0	0.0	0.0	0.023974896872686245	0.0
28	0.0	0.0	0.0	0.0747452667207277	0.0
29	0.0	0.0	0.0	0.15019567746712267	0.0
30	0.0	0.0	0.0	0.24186440080386418	0.0
31	0.0	0.0	0.0	0.4703310651200508	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACTG	20	0.0018365002	37.0	5
GGTATCA	35	2.3736806E-5	31.714285	1
CATCTGG	40	0.0019228949	23.125002	23
TAAAAGA	50	2.68595E-4	22.199999	4
CGTGACG	45	0.003809538	20.555555	36
TAGAGCT	50	0.007005253	18.499998	4
ATCGTGA	50	0.007005253	18.499998	34
TAAAGGT	65	0.0015707186	17.076923	3
CATCGTG	65	0.0015707186	17.076923	33
GGACAGT	65	0.0015707186	17.076923	6
GTATCAA	70	0.002577498	15.857142	1
GGACACA	70	0.002577498	15.857142	6
GACACAG	120	1.9064628E-6	15.416666	7
AGGACAC	85	5.323088E-4	15.235293	5
TCAAGAC	85	5.323088E-4	15.235293	3
CTCTTAT	195	4.0017767E-11	15.179487	37
GTTGAAG	90	8.211131E-4	14.388888	21
TCTCTTA	335	0.0	14.358209	37
TGCCCCC	80	0.0062627867	13.875001	10
CAACGCA	80	0.0062627867	13.875001	5
>>END_MODULE