##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632354.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4976
Sequences flagged as poor quality	0
Sequence length	43
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64489549839228	34.0	31.0	34.0	31.0	34.0
2	32.80124598070739	34.0	31.0	34.0	31.0	34.0
3	32.77170418006431	34.0	31.0	34.0	31.0	34.0
4	36.30807877813505	37.0	37.0	37.0	35.0	37.0
5	36.16579581993569	37.0	35.0	37.0	35.0	37.0
6	36.18147106109325	37.0	35.0	37.0	35.0	37.0
7	36.190916398713824	37.0	36.0	37.0	35.0	37.0
8	36.181270096463024	37.0	36.0	37.0	35.0	37.0
9	37.99015273311897	39.0	38.0	39.0	35.0	39.0
10	37.81189710610933	39.0	38.0	39.0	35.0	39.0
11	37.94614147909968	39.0	38.0	39.0	35.0	39.0
12	37.91860932475884	39.0	38.0	39.0	35.0	39.0
13	38.01446945337621	39.0	38.0	39.0	35.0	39.0
14	39.37299035369775	40.0	39.0	41.0	37.0	41.0
15	39.38203376205788	40.0	39.0	41.0	37.0	41.0
16	39.237942122186496	40.0	39.0	41.0	36.0	41.0
17	39.31631832797428	40.0	39.0	41.0	36.0	41.0
18	39.320940514469456	40.0	39.0	41.0	36.0	41.0
19	39.30265273311897	40.0	39.0	41.0	36.0	41.0
20	39.27431672025723	40.0	39.0	41.0	36.0	41.0
21	39.23693729903537	40.0	39.0	41.0	36.0	41.0
22	39.220056270096464	40.0	39.0	41.0	36.0	41.0
23	39.17725080385852	40.0	39.0	41.0	36.0	41.0
24	39.16177652733119	40.0	39.0	41.0	36.0	41.0
25	39.08902733118971	40.0	39.0	41.0	36.0	41.0
26	38.9439308681672	40.0	39.0	41.0	35.0	41.0
27	38.890876205787784	40.0	38.0	41.0	35.0	41.0
28	38.83561093247589	40.0	38.0	41.0	35.0	41.0
29	38.64670418006431	40.0	38.0	41.0	35.0	41.0
30	38.61575562700965	40.0	38.0	41.0	34.0	41.0
31	38.53557073954984	40.0	38.0	41.0	34.0	41.0
32	38.429461414791	40.0	38.0	41.0	34.0	41.0
33	38.29742765273312	40.0	38.0	41.0	34.0	41.0
34	38.283159163987136	40.0	38.0	41.0	34.0	41.0
35	38.212620578778136	40.0	38.0	41.0	34.0	41.0
36	38.18971061093247	40.0	38.0	41.0	34.0	41.0
37	38.17825562700965	40.0	38.0	41.0	34.0	41.0
38	38.08400321543408	40.0	37.0	41.0	33.0	41.0
39	37.86515273311897	40.0	37.0	41.0	33.0	41.0
40	37.82958199356913	40.0	37.0	41.0	33.0	41.0
41	37.88102893890675	40.0	37.0	41.0	33.0	41.0
42	37.82395498392283	40.0	37.0	41.0	33.0	41.0
43	36.858520900321544	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	0.0
23	3.0
24	2.0
25	6.0
26	14.0
27	18.0
28	25.0
29	19.0
30	32.0
31	46.0
32	61.0
33	111.0
34	142.0
35	232.0
36	368.0
37	596.0
38	1269.0
39	2031.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.790192926045016	16.479099678456592	10.65112540192926	29.079581993569132
2	21.60369774919614	18.066720257234728	30.285369774919612	30.04421221864952
3	21.282154340836012	19.212218649517684	27.311093247588424	32.19453376205787
4	15.89630225080386	14.891479099678456	30.22508038585209	38.98713826366559
5	18.066720257234728	32.11414790996784	31.511254019292608	18.30787781350482
6	39.87138263665595	30.848070739549836	13.02250803858521	16.258038585209004
7	32.13424437299036	27.331189710610932	18.368167202572348	22.166398713826364
8	29.54180064308682	30.04421221864952	18.04662379421222	22.367363344051448
9	27.471864951768488	12.942122186495178	16.479099678456592	43.106913183279744
10	17.584405144694536	24.236334405144692	30.94855305466238	27.230707395498392
11	40.19292604501607	18.528938906752412	18.810289389067524	22.467845659163988
12	26.326366559485532	21.362540192926044	25.904340836012864	26.406752411575564
13	35.14871382636656	16.438906752411576	19.915594855305464	28.4967845659164
14	25.924437299035368	17.363344051446948	20.779742765273312	35.932475884244376
15	28.9991961414791	25.120578778135048	17.363344051446948	28.516881028938908
16	29.03938906752412	22.126205787781352	19.493569131832796	29.340836012861736
17	28.315916398713824	22.005627009646304	21.342443729903536	28.336012861736336
18	27.190514469453376	22.889871382636656	21.844855305466236	28.07475884244373
19	28.516881028938908	21.0008038585209	23.2516077170418	27.230707395498392
20	29.381028938906752	21.402733118971064	21.20176848874598	28.014469453376208
21	30.54662379421222	20.538585209003216	20.59887459807074	28.315916398713824
22	28.657556270096464	21.362540192926044	21.5032154340836	28.476688102893892
23	27.833601286173632	21.70418006430868	21.382636655948552	29.079581993569132
24	29.722668810289388	21.483118971061092	21.0008038585209	27.79340836012862
25	29.903536977491964	20.759646302250804	20.739549839228296	28.597266881028936
26	29.099678456591636	21.905144694533764	20.518488745980708	28.476688102893892
27	28.416398713826368	20.679260450160772	21.82475884244373	29.079581993569132
28	28.69774919614148	21.643890675241156	20.719453376205788	28.938906752411576
29	27.090032154340836	21.061093247588424	22.789389067524116	29.059485530546624
30	26.768488745980708	21.784565916398712	21.70418006430868	29.7427652733119
31	27.431672025723476	22.487942122186492	21.60369774919614	28.476688102893892
32	27.170418006430868	21.061093247588424	22.829581993569132	28.938906752411576
33	27.009646302250808	22.487942122186492	22.84967845659164	27.652733118971064
34	27.110128617363344	21.483118971061092	21.905144694533764	29.5016077170418
35	27.351286173633437	21.262057877813508	23.15112540192926	28.235530546623792
36	26.44694533762058	21.30225080385852	24.4975884244373	27.7532154340836
37	27.110128617363344	20.337620578778136	23.633440514469452	28.918810289389068
38	26.527331189710612	21.30225080385852	24.05546623794212	28.114951768488744
39	25.241157556270092	22.02572347266881	24.135852090032152	28.597266881028936
40	25.984726688102892	20.578778135048232	25.683279742765276	27.7532154340836
41	25.100482315112536	20.136655948553052	25.683279742765276	29.079581993569132
42	23.613344051446948	21.020900321543408	27.351286173633437	28.014469453376208
43	24.738745980707396	19.011254019292604	27.069935691318324	29.180064308681676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	0.5
9	0.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	4.5
16	7.0
17	5.5
18	4.0
19	4.0
20	5.0
21	6.0
22	6.5
23	7.0
24	5.5
25	4.0
26	4.0
27	10.0
28	16.0
29	24.5
30	33.0
31	36.0
32	39.0
33	39.0
34	47.5
35	56.0
36	70.0
37	84.0
38	106.5
39	129.0
40	129.0
41	155.5
42	182.0
43	201.5
44	221.0
45	247.5
46	274.0
47	274.0
48	290.5
49	307.0
50	313.5
51	320.0
52	362.5
53	405.0
54	405.0
55	424.5
56	444.0
57	439.5
58	435.0
59	440.0
60	445.0
61	445.0
62	411.0
63	377.0
64	374.0
65	371.0
66	315.0
67	259.0
68	259.0
69	226.0
70	193.0
71	163.0
72	133.0
73	101.0
74	69.0
75	69.0
76	61.5
77	54.0
78	49.5
79	45.0
80	35.5
81	26.0
82	26.0
83	20.0
84	14.0
85	8.5
86	3.0
87	3.0
88	3.0
89	3.0
90	4.5
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4976.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	98.33199356913184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.65113427345187	97.0056270096463
2	1.185366850602902	2.3311897106109325
3	0.08174943797261394	0.2411575562700965
4	0.06131207847946045	0.2411575562700965
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.020437359493153485	0.18086816720257234
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	9	0.18086816720257234	Illumina PCR Primer Index 11 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.02009646302250804	0.0
28	0.0	0.0	0.0	0.08038585209003216	0.0
29	0.0	0.0	0.0	0.14067524115755628	0.0
30	0.0	0.0	0.0	0.3215434083601286	0.0
31	0.0	0.0	0.0	0.542604501607717	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE