FastQCFastQC Report
Fri 10 Feb 2017
ERR1632353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1092650
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.10186244451562715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8650.024.167631
TTAGACT957.1431714E-921.4210534
CTTATAC8600.019.14534837
CTTAGAC801.6165519E-518.53
CGAATTA953.6060574E-617.52631615
GTCTAAT650.001579866317.0769231
CTCTATA650.001579866317.0769232
GTATCAA12300.016.9959352
TCGTTTA904.4456327E-516.44444530
GGACCGT904.4456327E-516.4444456
CCAGTAC1708.54925E-1116.323533
TCTTATA15550.016.18006537
TAACGCC1201.93519E-615.4166664
GTACTAT1201.93519E-615.4166661
TCTATAC1455.343827E-815.3103463
TAGACAG1353.9713268E-715.0740735
GTGTAAT750.00410423414.81
CGCATCG1152.2095359E-514.47826113
AACGCCG1152.2095359E-514.4782615
GATTAGG1051.6555133E-414.0952381
Produced by FastQC (version 0.11.5)