##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1092650
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67954971857411	34.0	31.0	34.0	31.0	34.0
2	32.84685123324029	34.0	31.0	34.0	31.0	34.0
3	32.8503747769185	34.0	31.0	34.0	31.0	34.0
4	36.324984212693906	37.0	37.0	37.0	35.0	37.0
5	36.23576259552464	37.0	37.0	37.0	35.0	37.0
6	36.265755731478514	37.0	37.0	37.0	35.0	37.0
7	36.259356610076416	37.0	37.0	37.0	35.0	37.0
8	36.24060861208987	37.0	36.0	37.0	35.0	37.0
9	38.007624582437195	39.0	38.0	39.0	35.0	39.0
10	37.93686633414177	39.0	38.0	39.0	35.0	39.0
11	38.04858829451334	39.0	38.0	39.0	35.0	39.0
12	38.01574978263854	39.0	38.0	39.0	35.0	39.0
13	38.03911682606507	39.0	38.0	39.0	35.0	39.0
14	39.43034549032169	40.0	39.0	41.0	37.0	41.0
15	39.45056422459159	40.0	39.0	41.0	37.0	41.0
16	39.330327186198694	40.0	39.0	41.0	37.0	41.0
17	39.381933830595344	40.0	39.0	41.0	37.0	41.0
18	39.39268841806617	40.0	39.0	41.0	37.0	41.0
19	39.447145014414495	40.0	39.0	41.0	37.0	41.0
20	39.42662517732119	40.0	39.0	41.0	37.0	41.0
21	39.4074021873427	40.0	39.0	41.0	37.0	41.0
22	39.38198508213975	40.0	39.0	41.0	37.0	41.0
23	39.34359035372718	40.0	39.0	41.0	36.0	41.0
24	39.28728961698623	40.0	39.0	41.0	36.0	41.0
25	39.229655424884456	40.0	39.0	41.0	36.0	41.0
26	39.14782867340868	40.0	39.0	41.0	36.0	41.0
27	39.08279229396422	40.0	39.0	41.0	36.0	41.0
28	39.02187068137098	40.0	39.0	41.0	35.0	41.0
29	38.884309705761225	40.0	39.0	41.0	35.0	41.0
30	38.84376790372031	40.0	38.0	41.0	35.0	41.0
31	38.78331945270672	40.0	38.0	41.0	35.0	41.0
32	38.72249576717156	40.0	38.0	41.0	35.0	41.0
33	38.58769596851691	40.0	38.0	41.0	35.0	41.0
34	38.58943760582071	40.0	38.0	41.0	35.0	41.0
35	38.56622980826431	40.0	38.0	41.0	35.0	41.0
36	38.54001189767995	40.0	38.0	41.0	35.0	41.0
37	38.511546240790736	40.0	38.0	41.0	35.0	41.0
38	38.4513760124468	40.0	38.0	41.0	34.0	41.0
39	38.2693808630394	40.0	38.0	41.0	34.0	41.0
40	38.27354047499199	40.0	38.0	41.0	34.0	41.0
41	38.276664988788724	40.0	38.0	41.0	34.0	41.0
42	38.15404566878689	40.0	38.0	41.0	34.0	41.0
43	37.325674278131146	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	1.0
16	3.0
17	8.0
18	16.0
19	39.0
20	67.0
21	145.0
22	256.0
23	447.0
24	684.0
25	1106.0
26	1643.0
27	2384.0
28	3547.0
29	5009.0
30	7036.0
31	9757.0
32	13486.0
33	18701.0
34	27460.0
35	41638.0
36	65696.0
37	119018.0
38	282281.0
39	492220.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.64302384112021	18.172607879924954	12.252962979911224	26.93140529904361
2	19.593739989932732	19.83819155264723	33.564178831281744	27.00388962613829
3	20.49411980048506	21.406397290989794	29.14931588340274	28.950167025122408
4	15.273417837367868	15.351301880748638	33.43376195488034	35.94151832700316
5	16.11101450601748	34.841074452020315	33.05321923763327	15.994691804328925
6	35.693222898457876	33.71628609344255	15.19388642291676	15.396604585182812
7	30.37624124834119	29.243856678716877	20.316020683658994	20.063881389282937
8	28.141582391433673	32.18926463185833	19.33318079897497	20.335972177733034
9	27.332082551594745	13.717841943897863	18.842630302475634	40.107445202031755
10	17.785841760856634	25.855855031345808	32.147622752024894	24.210680455772664
11	37.09897954514254	20.715050565139798	20.335056971582848	21.85091291813481
12	22.953095684803003	24.492472429414725	27.566558367272226	24.987873518510046
13	31.87882670571546	18.696654921521073	23.0394911453805	26.385027227382967
14	23.882945133391296	19.77092390060861	23.738800164737107	32.60733080126298
15	26.82926829268293	26.22349334187526	20.824326179471925	26.122912185969888
16	26.24252962979911	24.868073033450784	22.55964856083833	26.329748775911778
17	24.894705532421177	25.223081499107675	24.147714272639913	25.73449869583124
18	24.988422642200156	24.01638219008832	24.846382647691392	26.148812520020137
19	25.869308561753535	24.32517274516085	24.721823090651167	25.083695602434446
20	26.175078936530454	24.056834301926507	24.489818331579187	25.278268429963852
21	26.7985173660367	24.090788450098387	23.507527570585275	25.603166613279644
22	27.004530270443418	24.05738342561662	23.508076694275385	25.430009609664577
23	26.23621470736283	24.045851828124285	23.62998215348007	26.08795131103281
24	25.944904589758842	24.168764014094172	23.742003386262756	26.144328009884227
25	26.067084610808582	23.623484189813755	24.02251407129456	26.286917128083097
26	25.882029927241113	24.018578684848762	24.011989200567427	26.0874021873427
27	25.908753946826522	24.055278451471192	23.904910080995744	26.13105752070654
28	25.502585457374273	23.99633917539926	24.501441449686542	25.999633917539928
29	25.442181851462042	24.214981924678533	24.6849402827987	25.657895941060726
30	25.21694961790143	24.744428682560745	24.169313137784286	25.869308561753535
31	25.78474351347641	24.41806616940466	23.9614698210772	25.83572049604173
32	25.036562485699903	24.261108314647874	24.467304260284628	26.23502493936759
33	25.062279778520114	24.21022285269757	24.588202992724113	26.13929437605821
34	25.621104653823274	23.521347183453074	24.738937445659634	26.118610717064016
35	25.245321008557177	23.717292820207753	25.272960234292775	25.7644259369423
36	25.045806067816777	23.720679082963436	24.860659863634282	26.372854985585505
37	24.951082231272594	22.810689607834163	25.58239143367043	26.65583672722281
38	24.451196632041366	22.89342424381092	26.341554935249167	26.31382418889855
39	24.049146570264952	22.76319040863955	27.028417150963254	26.159245870132246
40	23.756097560975608	22.207568754862034	28.022239509449502	26.014094174712852
41	23.23690111197547	22.16601839564362	28.43225186473253	26.16482862764838
42	22.936255891639593	22.28325630348236	28.38063423786208	26.399853567015967
43	22.232096279687	21.77614057566467	28.982839884684026	27.008923259964305
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	34.5
2	39.0
3	98.0
4	157.0
5	157.0
6	200.5
7	244.0
8	255.5
9	267.0
10	415.5
11	564.0
12	564.0
13	935.0
14	1306.0
15	2018.5
16	2731.0
17	2789.0
18	2847.0
19	2847.0
20	3581.0
21	4315.0
22	4816.0
23	5317.0
24	6874.5
25	8432.0
26	8432.0
27	10158.0
28	11884.0
29	14676.0
30	17468.0
31	20800.0
32	24132.0
33	24132.0
34	28432.0
35	32732.0
36	37578.5
37	42425.0
38	45894.0
39	49363.0
40	49363.0
41	54120.5
42	58878.0
43	62695.5
44	66513.0
45	69510.0
46	72507.0
47	72507.0
48	74357.5
49	76208.0
50	78304.5
51	80401.0
52	82394.0
53	84387.0
54	84387.0
55	82940.0
56	81493.0
57	78351.5
58	75210.0
59	71114.5
60	67019.0
61	67019.0
62	63510.0
63	60001.0
64	53027.0
65	46053.0
66	40759.5
67	35466.0
68	35466.0
69	30756.0
70	26046.0
71	23004.5
72	19963.0
73	17465.0
74	14967.0
75	14967.0
76	12804.0
77	10641.0
78	8143.5
79	5646.0
80	4403.0
81	3160.0
82	3160.0
83	2534.5
84	1909.0
85	1470.0
86	1031.0
87	765.5
88	500.0
89	500.0
90	390.5
91	281.0
92	186.0
93	91.0
94	54.0
95	17.0
96	17.0
97	12.0
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1092650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.29541626941842
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.4325763759491	61.2797219174853
2	6.767758379399841	9.37949268222713
3	1.764678363380472	3.6685236531625747
4	0.7768336075184377	2.153240328202566
5	0.4559359662618373	1.5797136287156763
6	0.3160189064184242	1.313919700156265
7	0.22599224366853723	1.0962158619069788
8	0.16179980750660103	0.8969588010785352
9	0.12950256260048898	0.8076540585021571
>10	0.8738500254120953	11.742880721988543
>50	0.0716046739877701	3.3621981598690285
>100	0.0229191659819939	2.3948654354104266
>500	2.6496095720070445E-4	0.12843225587144277
>1k	2.6496095720070445E-4	0.19618279542335473
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.10186244451562715	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	9.152061501853292E-5	0.0	0.0	0.0
6	0.0	9.152061501853292E-5	0.0	0.0	0.0
7	0.0	9.152061501853292E-5	0.0	0.0	0.0
8	0.0	9.152061501853292E-5	0.0	0.0	0.0
9	0.0	9.152061501853292E-5	0.0	9.152061501853292E-5	0.0
10	0.0	9.152061501853292E-5	0.0	9.152061501853292E-5	0.0
11	1.8304123003706584E-4	1.8304123003706584E-4	0.0	9.152061501853292E-5	0.0
12	1.8304123003706584E-4	1.8304123003706584E-4	0.0	9.152061501853292E-5	0.0
13	1.8304123003706584E-4	1.8304123003706584E-4	0.0	9.152061501853292E-5	0.0
14	1.8304123003706584E-4	1.8304123003706584E-4	0.0	9.152061501853292E-5	0.0
15	1.8304123003706584E-4	1.8304123003706584E-4	0.0	1.8304123003706584E-4	0.0
16	1.8304123003706584E-4	1.8304123003706584E-4	0.0	7.321649201482633E-4	0.0
17	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.001189767995240928	0.0
18	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.0015558504553150596	0.0
19	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.0017388916853521255	0.0
20	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.0020134535304077244	0.0
21	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.002654097835537455	0.0
22	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.004209948290852515	0.0
23	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.006497963666315837	0.0
24	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.00942662334690889	0.0
25	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.012080721182446345	0.0
26	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.01565002516816913	0.0
27	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.03505239555209811	9.152061501853292E-5
28	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.09875074360499703	9.152061501853292E-5
29	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.19054592046858554	9.152061501853292E-5
30	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.31675284857914243	9.152061501853292E-5
31	1.8304123003706584E-4	1.8304123003706584E-4	0.0	0.71303711160939	9.152061501853292E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	865	0.0	24.16763	1
TTAGACT	95	7.1431714E-9	21.421053	4
CTTATAC	860	0.0	19.145348	37
CTTAGAC	80	1.6165519E-5	18.5	3
CGAATTA	95	3.6060574E-6	17.526316	15
GTCTAAT	65	0.0015798663	17.076923	1
CTCTATA	65	0.0015798663	17.076923	2
GTATCAA	1230	0.0	16.995935	2
TCGTTTA	90	4.4456327E-5	16.444445	30
GGACCGT	90	4.4456327E-5	16.444445	6
CCAGTAC	170	8.54925E-11	16.32353	3
TCTTATA	1555	0.0	16.180065	37
TAACGCC	120	1.93519E-6	15.416666	4
GTACTAT	120	1.93519E-6	15.416666	1
TCTATAC	145	5.343827E-8	15.310346	3
TAGACAG	135	3.9713268E-7	15.074073	5
GTGTAAT	75	0.004104234	14.8	1
CGCATCG	115	2.2095359E-5	14.478261	13
AACGCCG	115	2.2095359E-5	14.478261	5
GATTAGG	105	1.6555133E-4	14.095238	1
>>END_MODULE