##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359729
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.77406603304154	34.0	31.0	34.0	31.0	34.0
2	32.94662370840271	34.0	31.0	34.0	31.0	34.0
3	33.03387272085375	34.0	33.0	34.0	31.0	34.0
4	36.42197042773866	37.0	37.0	37.0	35.0	37.0
5	36.289292773170914	37.0	37.0	37.0	35.0	37.0
6	36.3069227112632	37.0	37.0	37.0	35.0	37.0
7	36.2818760789372	37.0	37.0	37.0	35.0	37.0
8	36.27182406756197	37.0	37.0	37.0	35.0	37.0
9	38.06715610918219	39.0	38.0	39.0	35.0	39.0
10	38.03964373180922	39.0	38.0	39.0	35.0	39.0
11	38.105660094126414	39.0	38.0	39.0	37.0	39.0
12	38.06758976896497	39.0	38.0	39.0	35.0	39.0
13	38.088213627480684	39.0	38.0	39.0	37.0	39.0
14	39.48310533762916	40.0	39.0	41.0	37.0	41.0
15	39.4859936229773	40.0	39.0	41.0	37.0	41.0
16	39.48527641641347	40.0	39.0	41.0	37.0	41.0
17	39.47161891312627	40.0	39.0	41.0	37.0	41.0
18	39.463276522048545	40.0	39.0	41.0	37.0	41.0
19	39.486443961982495	40.0	39.0	41.0	37.0	41.0
20	39.457099650014314	40.0	39.0	41.0	37.0	41.0
21	39.42357719283127	40.0	39.0	41.0	37.0	41.0
22	39.38430040391517	40.0	39.0	41.0	37.0	41.0
23	39.338666051388685	40.0	39.0	41.0	36.0	41.0
24	39.28888135235135	40.0	39.0	41.0	36.0	41.0
25	39.22864156073044	40.0	39.0	41.0	36.0	41.0
26	39.14966544259707	40.0	39.0	41.0	36.0	41.0
27	39.079854557180546	40.0	39.0	41.0	36.0	41.0
28	38.99743973935935	40.0	39.0	41.0	35.0	41.0
29	38.94282918530338	40.0	39.0	41.0	35.0	41.0
30	38.87217321928452	40.0	38.0	41.0	35.0	41.0
31	38.79681093267432	40.0	38.0	41.0	35.0	41.0
32	38.72110672200462	40.0	38.0	41.0	35.0	41.0
33	38.640262530960804	40.0	38.0	41.0	35.0	41.0
34	38.573209276983505	40.0	38.0	41.0	35.0	41.0
35	38.535669907068936	40.0	38.0	41.0	35.0	41.0
36	38.49367162502884	40.0	38.0	41.0	35.0	41.0
37	38.435892018714085	40.0	38.0	41.0	34.0	41.0
38	38.380870043838556	40.0	38.0	41.0	34.0	41.0
39	38.32792185228325	40.0	38.0	41.0	34.0	41.0
40	38.24883453933378	40.0	38.0	41.0	34.0	41.0
41	38.209718982901016	40.0	38.0	41.0	34.0	41.0
42	38.14256287371883	40.0	37.0	41.0	34.0	41.0
43	37.25230659746643	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	4.0
19	16.0
20	16.0
21	38.0
22	76.0
23	144.0
24	237.0
25	360.0
26	501.0
27	729.0
28	1134.0
29	1612.0
30	2282.0
31	3124.0
32	4264.0
33	5973.0
34	8858.0
35	13543.0
36	21408.0
37	39037.0
38	91436.0
39	164936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54639186721115	17.444520736443266	11.76302160793264	27.246065788412942
2	20.632754101003812	19.398491642319634	31.93403923509086	28.034715021585694
3	21.00859257941395	20.623580528675753	28.052784179201566	30.315042712708735
4	15.947282537688093	14.857573339930893	31.949328522304288	37.245815600076725
5	17.37557995046271	33.277550600591	32.19618101404112	17.150688434905163
6	37.404268213015904	31.92903546836646	14.732479171821009	15.934217146796618
7	31.313294174225597	27.723925510592696	20.025630405110515	20.93714991007119
8	28.53258981066303	30.85294763558123	18.972615496665547	21.6418470570902
9	27.056200639926164	13.557427952708846	18.64459078917741	40.741780618187576
10	18.67516936360427	24.59740526896636	31.26909423482677	25.458331132602595
11	38.48230195508285	19.816306163806644	19.90303812036283	21.798353760747673
12	23.337845989619964	23.45988230028716	27.04118933975298	26.161082370339894
13	32.23231932927287	17.899029547242506	22.561150199177714	27.30750092430691
14	24.355834530994162	19.271729551968285	22.61785955538753	33.75457636165002
15	27.150438246568946	25.689894337126002	20.22411315184486	26.935554264460194
16	27.521828932335175	23.764000122314297	22.202268930222473	26.51190201512805
17	26.080744115709326	24.07367768514632	23.231654940246685	26.61392325889767
18	26.370962585724257	22.916417636609783	24.117877624545144	26.59474215312082
19	26.713998593385575	23.46738795037375	23.637516019003193	26.181097437237476
20	26.859107828393043	23.02594453046599	23.619724848427566	26.495222792713403
21	27.53294841394494	23.22720714760278	23.261121566512568	25.97872287193971
22	27.59466153687915	22.962285498250072	22.977296798423257	26.46575616644752
23	26.66090306869894	22.820234120685292	23.43458547962494	27.08427733099083
24	26.561105721251277	23.427357816578592	23.187177013807617	26.824359448362518
25	26.8682814007211	22.706537421225423	23.428747751779813	26.99643342627367
26	26.660347094618448	23.665592710067855	23.353135276833395	26.320924918480298
27	26.833533020690574	22.927537118219547	23.317274948641895	26.921654912447984
28	26.247258352815596	23.14297707440879	23.886036432981495	26.723728139794122
29	26.060729048811744	23.263345462834522	23.955255206002295	26.72067028235144
30	25.869751952163988	23.696727258575205	23.937464035426668	26.496056753834136
31	26.754862688301472	23.36203086212121	23.26834922955892	26.614757220018404
32	25.96287761064579	23.154374543058804	23.501580356323785	27.38116748997162
33	25.610390043616167	22.891676790028047	24.290785563577025	27.207147602778758
34	26.424336097451135	22.434110121786123	24.012520536292598	27.12903324447014
35	26.108820806773984	22.60729604785825	24.670515860550584	26.61336728481718
36	25.556460557808798	22.736560021571794	24.644663065807872	27.06231635481154
37	25.683222648160143	21.740532456376886	25.418023011767193	27.15822188369578
38	25.009938036688727	21.600704975134057	26.36456888379864	27.024788104378572
39	24.924040041253274	21.264896630519083	26.783495353446625	27.027567974781014
40	24.581004033591956	21.544273605964488	27.194916173008014	26.67980618743554
41	23.71312849394961	21.52314659090593	27.954654753995367	26.809070161149084
42	23.74231713317525	21.91788818805267	28.03832885310887	26.301465825663207
43	23.059024988255047	21.478390677426617	28.19844938828952	27.26413494602882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	15.0
2	17.0
3	38.5
4	60.0
5	60.0
6	73.0
7	86.0
8	90.0
9	94.0
10	123.5
11	153.0
12	153.0
13	271.5
14	390.0
15	582.5
16	775.0
17	782.0
18	789.0
19	789.0
20	946.0
21	1103.0
22	1121.5
23	1140.0
24	1440.0
25	1740.0
26	1740.0
27	2117.5
28	2495.0
29	3139.5
30	3784.0
31	4573.0
32	5362.0
33	5362.0
34	6669.5
35	7977.0
36	9369.5
37	10762.0
38	12411.5
39	14061.0
40	14061.0
41	15651.0
42	17241.0
43	18566.5
44	19892.0
45	21340.5
46	22789.0
47	22789.0
48	24052.0
49	25315.0
50	26464.5
51	27614.0
52	28492.5
53	29371.0
54	29371.0
55	29106.5
56	28842.0
57	28242.0
58	27642.0
59	26942.0
60	26242.0
61	26242.0
62	24183.5
63	22125.0
64	20114.5
65	18104.0
66	16039.0
67	13974.0
68	13974.0
69	12194.5
70	10415.0
71	8890.0
72	7365.0
73	5940.5
74	4516.0
75	4516.0
76	3678.5
77	2841.0
78	2292.5
79	1744.0
80	1500.5
81	1257.0
82	1257.0
83	971.0
84	685.0
85	587.5
86	490.0
87	386.0
88	282.0
89	282.0
90	211.0
91	140.0
92	86.5
93	33.0
94	20.5
95	8.0
96	8.0
97	4.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	359729.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.83271439853131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.4586890856556	66.0497784172822
2	6.060587144082646	8.94939199392929
3	1.7267551022420387	3.8247304890012948
4	0.830597682819351	2.453011259827321
5	0.46625849565151806	1.7212565172663692
6	0.3051700198851894	1.3518918552706383
7	0.19893057840321826	1.0281309206265619
8	0.16425935809504377	0.9702171418813959
9	0.12090208496238747	0.8033876198293427
>10	0.5999362437013804	8.314171280121945
>50	0.04829454542435662	2.4714878585025546
>100	0.019619659077300594	2.062544646461071
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	2.779870402441838E-4	0.0
16	0.0	0.0	0.0	5.559740804883676E-4	0.0
17	0.0	0.0	0.0	0.0013899352012209192	0.0
18	0.0	0.0	0.0	0.0013899352012209192	0.0
19	0.0	0.0	0.0	0.001667922241465103	0.0
20	0.0	0.0	0.0	0.0019459092817092868	0.0
21	0.0	0.0	0.0	0.0022238963219534703	0.0
22	0.0	0.0	0.0	0.0036138315231743897	0.0
23	0.0	0.0	0.0	0.00583772784512786	0.0
24	0.0	0.0	0.0	0.009729546408546433	0.0
25	0.0	0.0	0.0	0.014733313132941742	0.0
26	0.0	0.0	0.0	0.019181105776848683	0.0
27	0.0	0.0	0.0	0.05198357652566237	0.0
28	0.0	0.0	0.0	0.1339897533976966	0.0
29	0.0	0.0	0.0	0.24296067317341666	0.0
30	0.0	0.0	0.0	0.3872359470601481	0.0
31	0.0	0.0	0.0	0.7602945550678427	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCGT	55	1.897951E-5	23.545454	9
TACACCC	40	0.0019286227	23.125	4
ACCGTCG	50	2.6971032E-4	22.2	8
ATACCGT	50	2.6971032E-4	22.2	6
TACCGTC	50	2.6971032E-4	22.2	7
AGTACCA	70	5.08586E-6	21.142857	5
CGCGGGA	45	0.0038207762	20.555555	20
TAAAGCT	65	6.885293E-5	19.923077	4
ATAGGAC	75	9.236779E-6	19.733334	3
CGCGGTT	70	1.2162447E-4	18.5	10
ACTCTTT	60	9.2200097E-4	18.5	8
ATACTGC	50	0.0070257164	18.5	6
CGCAGCT	50	0.0070257164	18.5	31
GATGCCT	90	2.1438627E-6	18.5	37
CGTCGTA	60	9.2200097E-4	18.5	10
TCTTTCG	50	0.0070257164	18.5	10
TGAGCTA	50	0.0070257164	18.5	10
TAGGACC	85	2.7147038E-5	17.411764	4
GGTTCTA	75	2.0622952E-4	17.266666	13
TAGTAGT	75	2.0622952E-4	17.266666	4
>>END_MODULE