FastQCFastQC Report
Fri 10 Feb 2017
ERR1632351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418113
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33830.13990247767577446No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32080.13266542961391797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14150.022.6183741
CTTATAC25000.018.537
CTAGTAC803.3844967E-416.18753
TCTTATA42750.015.23274837
GTATCAA21800.014.596332
CGAATTA908.2786725E-414.38888815
TAGAACA2201.8189894E-1114.2954544
GTTAGAC950.001245769113.6315793
ACAACGC1254.8487043E-513.328
ATACACA8300.013.15060237
TATACTA3000.012.955
CTCTTAT64050.012.79547137
GTACTAG1451.3733454E-512.758621
CGTGCGA1653.8078542E-612.33333410
AATCGTA1359.9688485E-512.33333418
CTATACA2255.7134457E-912.3333334
GTACTAT2552.237357E-1012.3333331
ACCGTTT1050.002645024312.3333338
TATACAC9150.011.92896336
CGAATCG1401.39953E-411.89285716
Produced by FastQC (version 0.11.5)