##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2418113
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52702044941655	34.0	31.0	34.0	31.0	34.0
2	32.70635408684375	34.0	31.0	34.0	31.0	34.0
3	32.79375116051235	34.0	31.0	34.0	31.0	34.0
4	36.25914669827258	37.0	37.0	37.0	35.0	37.0
5	36.09436821190739	37.0	35.0	37.0	35.0	37.0
6	36.11444212904856	37.0	35.0	37.0	35.0	37.0
7	36.09294478794002	37.0	35.0	37.0	35.0	37.0
8	36.077286297207785	37.0	35.0	37.0	35.0	37.0
9	37.82378656415147	39.0	38.0	39.0	35.0	39.0
10	37.77207144579265	39.0	37.0	39.0	35.0	39.0
11	37.846317355723244	39.0	38.0	39.0	35.0	39.0
12	37.804460337461485	39.0	37.0	39.0	35.0	39.0
13	37.828732569569745	39.0	38.0	39.0	35.0	39.0
14	39.17267059066305	40.0	39.0	41.0	36.0	41.0
15	39.169447829774704	40.0	39.0	41.0	36.0	41.0
16	39.163742141082736	40.0	39.0	41.0	36.0	41.0
17	39.14307685372851	40.0	39.0	41.0	36.0	41.0
18	39.135256706365666	40.0	39.0	41.0	36.0	41.0
19	39.16361476903685	40.0	39.0	41.0	36.0	41.0
20	39.136147897141285	40.0	39.0	41.0	36.0	41.0
21	39.112525345176174	40.0	39.0	41.0	36.0	41.0
22	39.06678099824119	40.0	39.0	41.0	36.0	41.0
23	39.020874127884014	40.0	39.0	41.0	36.0	41.0
24	38.9715344154719	40.0	38.0	41.0	35.0	41.0
25	38.91231799341057	40.0	38.0	41.0	35.0	41.0
26	38.814688560873705	40.0	38.0	41.0	35.0	41.0
27	38.734785347086756	40.0	38.0	41.0	35.0	41.0
28	38.67301528092359	40.0	38.0	41.0	35.0	41.0
29	38.596538706007536	40.0	38.0	41.0	35.0	41.0
30	38.531346963520726	40.0	38.0	41.0	35.0	41.0
31	38.45226877321283	40.0	38.0	41.0	34.0	41.0
32	38.36871477883788	40.0	38.0	41.0	34.0	41.0
33	38.29606267366331	40.0	38.0	41.0	34.0	41.0
34	38.21739720186774	40.0	38.0	41.0	34.0	41.0
35	38.19223626025748	40.0	38.0	41.0	34.0	41.0
36	38.15467391308843	40.0	38.0	41.0	34.0	41.0
37	38.10317466553465	40.0	38.0	41.0	34.0	41.0
38	38.05310628576911	40.0	38.0	41.0	33.0	41.0
39	37.99618173344256	40.0	37.0	41.0	33.0	41.0
40	37.93171410930755	40.0	37.0	41.0	33.0	41.0
41	37.879503563315694	40.0	37.0	41.0	33.0	41.0
42	37.82482952616358	40.0	37.0	41.0	33.0	41.0
43	36.94481151211709	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	2.0
12	1.0
13	2.0
14	1.0
15	3.0
16	5.0
17	12.0
18	43.0
19	102.0
20	167.0
21	353.0
22	654.0
23	1136.0
24	1906.0
25	2981.0
26	4649.0
27	6807.0
28	10129.0
29	14587.0
30	20217.0
31	27888.0
32	37553.0
33	51327.0
34	73455.0
35	107641.0
36	166602.0
37	300903.0
38	694276.0
39	894709.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.387597271095274	18.060901206850136	12.484486870547407	27.067014651507186
2	19.677409616506754	19.49681425144317	33.79457452980898	27.031201602241083
3	20.933430323562217	20.966389908163926	28.190700765431558	29.9094790028423
4	16.1262521643943	15.243456364528871	32.13034295750447	36.49994851357236
5	17.20386929808491	33.644333412044844	32.225044900713904	16.926752389156338
6	36.52575375923292	32.38301932126414	15.027254723000953	16.063972196501982
7	30.93713982762592	28.294459357358402	20.178875015352883	20.589525799662795
8	27.660204465217298	32.33666085910791	19.19385074229368	20.809283933381113
9	26.908709394474123	14.303798044177423	19.18950851345657	39.597984047891885
10	18.424366437796742	25.425279960034953	31.77229517396416	24.37805842820414
11	37.503127438626734	20.524144239743965	20.268697120440606	21.704031201188695
12	22.793723866502518	23.946771718277848	27.8761579793831	25.38334643583654
13	31.86848588134632	18.728281101834366	22.856003834394837	26.547229182424477
14	23.886352705601436	19.602681925948044	23.602453648774894	32.90851171967563
15	26.568278653644388	25.94692638433357	21.04971934727616	26.43507561474588
16	26.433504141452445	24.566138968691703	22.890204055807153	26.1101528340487
17	25.104037735209232	24.96690601307714	24.142047952266914	25.78700829944672
18	25.514068201113844	23.98308102226819	24.811743702630938	25.69110707398703
19	26.045226174293756	24.12414142763386	24.69950742583163	25.131124972240755
20	26.090219935958324	23.96364437890206	24.4980693623499	25.448066322789714
21	26.476430175099342	23.889371588507235	24.06934663516552	25.5648516012279
22	26.630889458019542	23.79702685523795	23.942843035044266	25.629240651698243
23	26.14861257517742	23.7517436116509	24.10168590136193	25.997957911809742
24	25.95155809509316	24.029439484424426	23.887221151368855	26.131781269113564
25	26.24827706562927	23.550388257289878	24.04081198852163	26.16052268855922
26	25.89171804626169	24.02517996470802	24.1735187726959	25.909583216334394
27	25.91495930918034	23.855295430775982	24.083572603927113	26.14617265611657
28	25.716416064923354	23.94904621909729	24.41850318822983	25.91603452774953
29	25.4771385787182	24.13510038612753	24.63090021020523	25.75686082494904
30	25.611913090910143	24.20110226445166	24.30606013862876	25.88092450600944
31	25.893661710598302	23.89574019080167	24.192335097656724	26.0182630009433
32	25.233188027193105	23.94991466486471	24.459816394022944	26.35708091391924
33	25.344762631026757	23.555598931894416	24.825721544030408	26.273916893048423
34	25.81517075504743	23.062776636162162	24.82398465249556	26.298067956294847
35	25.26941462206274	23.177328768341265	25.353736570623457	26.19952003897254
36	25.090266666611527	22.891072501574573	25.474574595976286	26.544086235837618
37	24.930265872603968	22.061417311763346	26.168793600629915	26.839523215002774
38	24.241671088158412	22.063691812582785	27.183220966100425	26.511416133158377
39	24.071207590381423	21.72032489796796	27.72657853458461	26.481888977066003
40	23.725028565662566	21.839591450027356	28.243675957244346	26.191704027065732
41	23.10342816898962	21.818790106169565	28.730046941561454	26.347734783279357
42	22.74016971084478	22.197349751645188	28.760483897981608	26.301996639528426
43	22.289322293871294	22.028457727161634	28.972508728913827	26.709711250053243
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	143.5
2	162.0
3	429.0
4	696.0
5	696.0
6	838.0
7	980.0
8	948.0
9	916.0
10	1346.0
11	1776.0
12	1776.0
13	3091.0
14	4406.0
15	6133.0
16	7860.0
17	8008.5
18	8157.0
19	8157.0
20	10279.5
21	12402.0
22	12703.5
23	13005.0
24	15833.0
25	18661.0
26	18661.0
27	22197.5
28	25734.0
29	31188.5
30	36643.0
31	43152.5
32	49662.0
33	49662.0
34	58841.5
35	68021.0
36	76786.5
37	85552.0
38	94886.0
39	104220.0
40	104220.0
41	113308.0
42	122396.0
43	129932.0
44	137468.0
45	145077.0
46	152686.0
47	152686.0
48	159825.0
49	166964.0
50	171529.5
51	176095.0
52	179738.0
53	183381.0
54	183381.0
55	182445.0
56	181509.0
57	177585.5
58	173662.0
59	168129.0
60	162596.0
61	162596.0
62	151050.0
63	139504.0
64	125790.0
65	112076.0
66	99239.0
67	86402.0
68	86402.0
69	74765.5
70	63129.0
71	54278.5
72	45428.0
73	37215.5
74	29003.0
75	29003.0
76	23934.0
77	18865.0
78	15410.5
79	11956.0
80	9744.5
81	7533.0
82	7533.0
83	5873.0
84	4213.0
85	3266.5
86	2320.0
87	1786.0
88	1252.0
89	1252.0
90	890.0
91	528.0
92	323.5
93	119.0
94	77.5
95	36.0
96	36.0
97	24.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2418113.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.78848836978418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.15648404458334	57.726293282679485
2	8.808080287011917	11.941728961924651
3	2.5091478848175006	5.102740266240591
4	1.0694768928409724	2.899928876484127
5	0.6205535339879483	2.103319301078525
6	0.3700036475130392	1.504919277372923
7	0.2539027844863594	1.2048180167246576
8	0.18000576155970915	0.976185477918759
9	0.1507512165024476	0.9197277377946286
>10	0.8029968184578801	10.046203800666442
>50	0.055245468310780727	2.556921640495668
>100	0.022924858658478302	2.520323269837586
>500	1.8291482986212195E-4	0.06993329176644343
>1k	2.438864398112616E-4	0.4269567990155167
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3383	0.13990247767577446	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3208	0.13266542961391797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	8.27091207069314E-5	0.0	0.0	0.0
6	0.0	8.27091207069314E-5	0.0	0.0	0.0
7	0.0	8.27091207069314E-5	0.0	0.0	0.0
8	0.0	8.27091207069314E-5	0.0	0.0	0.0
9	0.0	8.27091207069314E-5	0.0	4.13545603534657E-5	0.0
10	0.0	8.27091207069314E-5	0.0	8.27091207069314E-5	0.0
11	0.0	8.27091207069314E-5	0.0	1.240636810603971E-4	0.0
12	0.0	8.27091207069314E-5	0.0	1.240636810603971E-4	0.0
13	0.0	8.27091207069314E-5	0.0	1.240636810603971E-4	0.0
14	0.0	8.27091207069314E-5	0.0	1.654182414138628E-4	0.0
15	0.0	8.27091207069314E-5	0.0	1.654182414138628E-4	0.0
16	0.0	8.27091207069314E-5	0.0	2.8948192247425986E-4	0.0
17	0.0	8.27091207069314E-5	0.0	7.857366467158483E-4	0.0
18	0.0	8.27091207069314E-5	0.0	9.511548881297111E-4	0.0
19	0.0	8.27091207069314E-5	0.0	9.511548881297111E-4	0.0
20	0.0	8.27091207069314E-5	0.0	0.0010338640088366424	0.0
21	0.0	8.27091207069314E-5	0.0	0.0012819913709574365	0.0
22	0.0	8.27091207069314E-5	0.0	0.0019023097762594222	0.0
23	0.0	1.240636810603971E-4	0.0	0.002605337302268339	0.0
24	0.0	1.654182414138628E-4	0.0	0.004300874276760433	0.0
25	0.0	1.654182414138628E-4	0.0	0.006575375096201046	0.0
26	0.0	1.654182414138628E-4	0.0	0.01153792233861693	0.0
27	0.0	1.654182414138628E-4	0.0	0.03941089601685281	0.0
28	0.0	2.0677280176732848E-4	0.0	0.12857132813892486	0.0
29	0.0	2.481273621207942E-4	0.0	0.23873987692055748	0.0
30	0.0	2.481273621207942E-4	0.0	0.3913382046248459	0.0
31	0.0	2.481273621207942E-4	0.0	0.8741113421912045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1415	0.0	22.618374	1
CTTATAC	2500	0.0	18.5	37
CTAGTAC	80	3.3844967E-4	16.1875	3
TCTTATA	4275	0.0	15.232748	37
GTATCAA	2180	0.0	14.59633	2
CGAATTA	90	8.2786725E-4	14.388888	15
TAGAACA	220	1.8189894E-11	14.295454	4
GTTAGAC	95	0.0012457691	13.631579	3
ACAACGC	125	4.8487043E-5	13.32	8
ATACACA	830	0.0	13.150602	37
TATACTA	300	0.0	12.95	5
CTCTTAT	6405	0.0	12.795471	37
GTACTAG	145	1.3733454E-5	12.75862	1
CGTGCGA	165	3.8078542E-6	12.333334	10
AATCGTA	135	9.9688485E-5	12.333334	18
CTATACA	225	5.7134457E-9	12.333333	4
GTACTAT	255	2.237357E-10	12.333333	1
ACCGTTT	105	0.0026450243	12.333333	8
TATACAC	915	0.0	11.928963	36
CGAATCG	140	1.39953E-4	11.892857	16
>>END_MODULE