FastQCFastQC Report
Fri 10 Feb 2017
ERR1632350.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632350.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452480
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4570.10099893917963226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACG358.861051E-426.428579
CTAGAAC751.3689714E-824.6666663
TCTAGCG551.8993549E-523.54545428
TATCACT400.001929387823.1254
GTAATGG400.001929387823.1251
TTATACA1900.021.42105337
GGTATCA3000.020.351
GTCTAGA555.136472E-420.1818181
CTAAAGG555.136472E-420.1818183
CTTATAC4050.019.18518437
CCTAGAG500.007028451718.53
GCGAAAG609.2250184E-418.518
GAACTCT500.007028451718.56
TACCTCA500.007028451718.57
GACGGAC609.2250184E-418.57
CATACAC500.007028451718.53
TATACAC701.217125E-418.537
TAAGCCG500.007028451718.529
CTAGCGG701.217125E-418.529
GGACTGC852.717211E-517.4117646
Produced by FastQC (version 0.11.5)