##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632350.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452480
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.81881188118812	34.0	31.0	34.0	31.0	34.0
2	32.98813649222065	34.0	33.0	34.0	31.0	34.0
3	33.07397896039604	34.0	33.0	34.0	31.0	34.0
4	36.445491513437055	37.0	37.0	37.0	35.0	37.0
5	36.32239214992928	37.0	37.0	37.0	35.0	37.0
6	36.34237756364922	37.0	37.0	37.0	35.0	37.0
7	36.322129154879775	37.0	37.0	37.0	35.0	37.0
8	36.310031382602546	37.0	37.0	37.0	35.0	37.0
9	38.11758530763791	39.0	39.0	39.0	37.0	39.0
10	38.07949964639321	39.0	38.0	39.0	37.0	39.0
11	38.14550919377652	39.0	39.0	39.0	37.0	39.0
12	38.11940638260255	39.0	38.0	39.0	37.0	39.0
13	38.13131630127298	39.0	39.0	39.0	37.0	39.0
14	39.53820279349363	41.0	39.0	41.0	37.0	41.0
15	39.54819660537483	41.0	39.0	41.0	37.0	41.0
16	39.55105419024045	41.0	39.0	41.0	37.0	41.0
17	39.536867927864215	41.0	39.0	41.0	37.0	41.0
18	39.53789338755304	41.0	39.0	41.0	37.0	41.0
19	39.5612270155587	41.0	39.0	41.0	37.0	41.0
20	39.53044554455445	41.0	39.0	41.0	37.0	41.0
21	39.50638039250354	41.0	39.0	41.0	37.0	41.0
22	39.471013083451204	41.0	39.0	41.0	37.0	41.0
23	39.4267039427157	40.0	39.0	41.0	37.0	41.0
24	39.366146570014145	41.0	39.0	41.0	37.0	41.0
25	39.31778200141443	40.0	39.0	41.0	36.0	41.0
26	39.25706992574258	40.0	39.0	41.0	36.0	41.0
27	39.179300742574256	40.0	39.0	41.0	36.0	41.0
28	39.11280277581329	40.0	39.0	41.0	36.0	41.0
29	39.05955401343706	40.0	39.0	41.0	36.0	41.0
30	38.99561085572843	40.0	39.0	41.0	35.0	41.0
31	38.935095473833094	40.0	38.0	41.0	35.0	41.0
32	38.860727545968885	40.0	38.0	41.0	35.0	41.0
33	38.78245226308345	40.0	38.0	41.0	35.0	41.0
34	38.71213755304102	40.0	38.0	41.0	35.0	41.0
35	38.69392459335219	40.0	38.0	41.0	35.0	41.0
36	38.66540841584158	40.0	38.0	41.0	35.0	41.0
37	38.615112270155585	40.0	38.0	41.0	35.0	41.0
38	38.56550786775106	40.0	38.0	41.0	35.0	41.0
39	38.51146349009901	40.0	38.0	41.0	35.0	41.0
40	38.441069218528995	40.0	38.0	41.0	34.0	41.0
41	38.38922604314003	40.0	38.0	41.0	34.0	41.0
42	38.343036156294204	40.0	38.0	41.0	34.0	41.0
43	37.47248497171146	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	3.0
18	3.0
19	5.0
20	18.0
21	52.0
22	71.0
23	105.0
24	238.0
25	353.0
26	551.0
27	830.0
28	1212.0
29	1757.0
30	2375.0
31	3482.0
32	4954.0
33	6948.0
34	10126.0
35	15676.0
36	25537.0
37	47149.0
38	114748.0
39	216286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.24279526166902	17.44916902404526	11.68427333804809	27.623762376237625
2	20.206417963224894	19.59114214992928	32.7338224893918	27.468617397454032
3	21.082699787835928	20.379464285714285	28.24058521923621	30.29725070721358
4	16.115408415841586	14.680648868458274	31.809803748231968	37.39413896746818
5	17.255348302687413	33.52877475247525	32.08716407355021	17.12871287128713
6	37.52408946251768	31.661067892503535	14.623408769448373	16.191433875530407
7	31.38790664780764	27.85559582743989	19.785846888260256	20.970650636492223
8	28.355065417256007	31.10855728429986	19.195765558698728	21.340611739745402
9	27.457567185289953	13.381585926449787	18.908239038189535	40.25260785007072
10	18.55021216407355	25.119342291371993	31.381276520509193	24.94916902404526
11	38.15594059405941	19.988286775106083	19.788277934936353	22.06749469589816
12	23.546013083451204	23.504243281471005	27.08628005657709	25.863463578500706
13	32.12318776520509	18.1095297029703	22.808521923620933	26.95876060820368
14	24.554013437057993	19.270243988684584	22.63503359264498	33.54070898161245
15	27.20274929278642	25.985899929278645	20.436704384724187	26.37464639321075
16	27.45425212164074	23.713755304101838	22.069925742574256	26.762066831683168
17	25.470518033946256	24.629375884016973	23.41805162659123	26.482054455445546
18	26.26060820367751	22.953721711456858	24.441522277227723	26.344147807637906
19	26.490894625176804	23.468219589816126	23.79795792079208	26.242927864214995
20	26.48647454031117	23.652537128712872	24.046587694483733	25.81440063649222
21	27.475026520509193	23.254950495049506	23.70800919377652	25.56201379066478
22	27.220429632248937	22.9568157708628	23.124336987270155	26.6984176096181
23	26.309229137199434	23.267105728429986	23.605021216407355	26.818643917963225
24	26.362933168316832	23.42976485148515	23.311969589816126	26.895332390381892
25	26.853562588401697	23.302908415841582	23.433521923620933	26.410007072135784
26	26.804720650636494	23.201467468175387	23.273735855728432	26.720076025459687
27	26.570898161244692	23.083451202263085	23.728120579915135	26.61753005657709
28	25.750972418670436	23.280586987270155	24.11465700141443	26.85378359264498
29	25.753403465346537	23.305781471004245	23.938958628005658	27.00185643564356
30	25.546543493635077	23.736739745403113	24.524840876944836	26.191875884016973
31	26.668140028288544	23.382248939179632	23.444130127298443	26.50548090523338
32	25.939047029702973	23.047206506364923	23.930560466760962	27.083185997171142
33	25.71030763790665	22.935157355021214	24.21852899575672	27.136006011315416
34	26.362049151343708	22.43657178217822	24.088578500707214	27.11280056577086
35	25.745668316831683	22.581771570014144	24.96972241867044	26.702837694483733
36	25.53328323903819	22.17092468175389	25.215479137199438	27.08031294200849
37	25.730861032531827	21.674549151343705	25.70522454031117	26.889365275813297
38	25.07602545968883	21.29795792079208	26.84649045261669	26.779526166902407
39	24.95491513437058	21.185024752475247	27.27766973125884	26.582390381895333
40	24.498541371994342	21.518741159830267	27.27634370579915	26.70637376237624
41	23.917300212164076	21.05617927864215	28.512420438472418	26.51410007072136
42	23.709556223479492	21.241822842998587	28.32743988684583	26.721181046676097
43	22.57337340876945	21.321384370579917	28.622922560113157	27.48231966053748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	18.0
2	21.0
3	36.0
4	51.0
5	51.0
6	80.0
7	109.0
8	107.5
9	106.0
10	159.0
11	212.0
12	212.0
13	375.0
14	538.0
15	774.5
16	1011.0
17	983.0
18	955.0
19	955.0
20	1189.0
21	1423.0
22	1497.0
23	1571.0
24	1876.5
25	2182.0
26	2182.0
27	2652.0
28	3122.0
29	3846.0
30	4570.0
31	5555.0
32	6540.0
33	6540.0
34	7989.5
35	9439.0
36	11419.5
37	13400.0
38	14974.5
39	16549.0
40	16549.0
41	18353.0
42	20157.0
43	22265.5
44	24374.0
45	27268.5
46	30163.0
47	30163.0
48	31789.5
49	33416.0
50	35033.5
51	36651.0
52	37623.5
53	38596.0
54	38596.0
55	38177.0
56	37758.0
57	36763.5
58	35769.0
59	35167.0
60	34565.0
61	34565.0
62	31896.0
63	29227.0
64	25701.0
65	22175.0
66	19789.0
67	17403.0
68	17403.0
69	14496.0
70	11589.0
71	9836.0
72	8083.0
73	6210.5
74	4338.0
75	4338.0
76	3383.0
77	2428.0
78	1959.0
79	1490.0
80	1292.0
81	1094.0
82	1094.0
83	848.5
84	603.0
85	502.5
86	402.0
87	315.5
88	229.0
89	229.0
90	178.5
91	128.0
92	73.0
93	18.0
94	13.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452480.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.18817502578634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.86395259476008	52.53858559774001
2	7.162183261027656	8.76481845885035
3	2.359242873024457	4.330732975288785
4	1.2184046812870875	2.982078355633269
5	0.6812860520966544	2.0843325099158543
6	0.45079288946662094	1.654991653263814
7	0.32485610720934427	1.391414664228468
8	0.24955703030621915	1.2215951399433924
9	0.20049238863378505	1.1040987030355813
>10	1.339894302970813	15.98263424110764
>50	0.11426331932726863	4.746962190062005
>100	0.03507449988995731	3.197755510930861
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	457	0.10099893917963226	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.21004243281471E-4	0.0
12	0.0	0.0	0.0	2.21004243281471E-4	0.0
13	0.0	0.0	0.0	2.21004243281471E-4	0.0
14	0.0	0.0	0.0	2.21004243281471E-4	0.0
15	0.0	0.0	0.0	2.21004243281471E-4	0.0
16	0.0	0.0	0.0	4.42008486562942E-4	0.0
17	0.0	0.0	0.0	0.001547029702970297	0.0
18	0.0	0.0	0.0	0.001768033946251768	0.0
19	0.0	0.0	0.0	0.00221004243281471	0.0
20	0.0	0.0	0.0	0.00221004243281471	0.0
21	0.0	0.0	0.0	0.003094059405940594	0.0
22	0.0	0.0	0.0	0.00442008486562942	0.0
23	0.0	0.0	0.0	0.005083097595473833	0.0
24	0.0	0.0	0.0	0.007735148514851485	0.0
25	0.0	0.0	0.0	0.009724186704384723	0.0
26	0.0	0.0	0.0	0.013702263083451202	0.0
27	0.0	0.0	0.0	0.03491867043847242	0.0
28	0.0	0.0	0.0	0.11779526166902404	0.0
29	0.0	0.0	0.0	0.22962340876944837	0.0
30	0.0	0.0	0.0	0.3761492220650636	0.0
31	0.0	0.0	0.0	0.7777139321074965	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCACG	35	8.861051E-4	26.42857	9
CTAGAAC	75	1.3689714E-8	24.666666	3
TCTAGCG	55	1.8993549E-5	23.545454	28
TATCACT	40	0.0019293878	23.125	4
GTAATGG	40	0.0019293878	23.125	1
TTATACA	190	0.0	21.421053	37
GGTATCA	300	0.0	20.35	1
GTCTAGA	55	5.136472E-4	20.181818	1
CTAAAGG	55	5.136472E-4	20.181818	3
CTTATAC	405	0.0	19.185184	37
CCTAGAG	50	0.0070284517	18.5	3
GCGAAAG	60	9.2250184E-4	18.5	18
GAACTCT	50	0.0070284517	18.5	6
TACCTCA	50	0.0070284517	18.5	7
GACGGAC	60	9.2250184E-4	18.5	7
CATACAC	50	0.0070284517	18.5	3
TATACAC	70	1.217125E-4	18.5	37
TAAGCCG	50	0.0070284517	18.5	29
CTAGCGG	70	1.217125E-4	18.5	29
GGACTGC	85	2.717211E-5	17.411764	6
>>END_MODULE