FastQCFastQC Report
Fri 10 Feb 2017
ERR1632349.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632349.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22926
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT580.25298787402948614TruSeq Adapter, Index 12 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC200.001804528137.035
CCGTCTT200.001804528137.037
GCCGTCT303.4914506E-430.83333436
TATGCCG250.005385979529.633
ATGCCGT250.005385979529.634
CGAGCCC400.001874305423.1255
TCCGAGC400.001874305423.1253
GTCTCTT450.00371418120.55555536
CCGAGCC450.00371418120.5555554
TCTCTTA450.00371418120.55555537
CTCCGAG450.00371418120.5555552
TGTCTCT1253.45608E-1019.2437
CTGTCTC1556.0554157E-915.516129536
Produced by FastQC (version 0.11.5)