##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632349.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	22926
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.768865044054785	34.0	31.0	34.0	31.0	34.0
2	32.95149611794469	34.0	31.0	34.0	31.0	34.0
3	33.03458954898369	34.0	33.0	34.0	31.0	34.0
4	36.42497600977057	37.0	37.0	37.0	35.0	37.0
5	36.29285527348861	37.0	37.0	37.0	35.0	37.0
6	36.33455465410451	37.0	37.0	37.0	35.0	37.0
7	36.30738026694583	37.0	37.0	37.0	35.0	37.0
8	36.27802494983861	37.0	37.0	37.0	35.0	37.0
9	38.0587542528134	39.0	38.0	39.0	35.0	39.0
10	38.07441332984384	39.0	38.0	39.0	35.0	39.0
11	38.135479368402684	39.0	38.0	39.0	37.0	39.0
12	38.08270086364826	39.0	38.0	39.0	35.0	39.0
13	38.10629852569136	39.0	38.0	39.0	37.0	39.0
14	39.50087237197941	40.0	39.0	41.0	37.0	41.0
15	39.49524557271221	40.0	39.0	41.0	37.0	41.0
16	39.498516967635	40.0	39.0	41.0	37.0	41.0
17	39.493762540347205	40.0	39.0	41.0	37.0	41.0
18	39.51011951496118	40.0	39.0	41.0	37.0	41.0
19	39.49507109831632	40.0	39.0	41.0	37.0	41.0
20	39.48006630027044	40.0	39.0	41.0	37.0	41.0
21	39.47090639448661	40.0	39.0	41.0	37.0	41.0
22	39.42406001919218	40.0	39.0	41.0	37.0	41.0
23	39.35675652098055	40.0	39.0	41.0	37.0	41.0
24	39.32412980895054	40.0	39.0	41.0	36.0	41.0
25	39.23916077815581	40.0	39.0	41.0	36.0	41.0
26	39.18084271133211	40.0	39.0	41.0	36.0	41.0
27	39.07306115327576	40.0	39.0	41.0	36.0	41.0
28	39.02324871325133	40.0	39.0	41.0	35.0	41.0
29	38.94338305853616	40.0	38.0	41.0	35.0	41.0
30	38.901029398935705	40.0	38.0	41.0	35.0	41.0
31	38.831501352176566	40.0	38.0	41.0	35.0	41.0
32	38.77680362906744	40.0	38.0	41.0	35.0	41.0
33	38.7272965192358	40.0	38.0	41.0	35.0	41.0
34	38.59783651749106	40.0	38.0	41.0	35.0	41.0
35	38.57410799965105	40.0	38.0	41.0	35.0	41.0
36	38.54340050597575	40.0	38.0	41.0	35.0	41.0
37	38.50942161737765	40.0	38.0	41.0	35.0	41.0
38	38.45027479717351	40.0	38.0	41.0	35.0	41.0
39	38.388554479630116	40.0	38.0	41.0	34.0	41.0
40	38.315580563552295	40.0	38.0	41.0	34.0	41.0
41	38.27776323824479	40.0	38.0	41.0	34.0	41.0
42	38.24914943732007	40.0	37.0	41.0	34.0	41.0
43	37.359548111314666	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	3.0
21	3.0
22	4.0
23	10.0
24	10.0
25	20.0
26	21.0
27	40.0
28	59.0
29	96.0
30	141.0
31	180.0
32	253.0
33	372.0
34	522.0
35	866.0
36	1401.0
37	2442.0
38	6157.0
39	10322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.50196283695368	17.783302800314054	12.627584401989008	27.08714996074326
2	21.24661955857978	19.798482072755824	31.196021983773882	27.75887638489052
3	21.573759050859287	20.09508854575591	28.631248364302536	29.699904039082263
4	16.374422053563638	15.13129198290151	31.51879961615633	36.97548634737852
5	17.508505626799266	33.499084009421615	31.793596789671113	17.198813574107998
6	37.983075983599406	31.470819157288666	14.577335775974875	15.96876908313705
7	31.745616330803454	26.960656023728518	19.545494198726338	21.74823344674169
8	28.491668847596614	29.922358893832328	19.23580214603507	22.350170112535984
9	26.764372328360814	13.390909883974528	18.023205094652358	41.8215126930123
10	18.437581784873068	24.880048852830846	31.283259181715085	25.399110180580998
11	38.7594870452761	19.846462531623484	18.616418040652537	22.777632382447877
12	23.554043444124577	23.35339788885981	26.001046846375292	27.091511820640317
13	32.91459478321556	17.364564250196285	22.38506499171247	27.335775974875688
14	24.906220012213208	19.71996859460874	21.22917211899154	34.14463927418651
15	28.31283259181715	25.591032016051646	19.144203088196807	26.951932303934395
16	28.295385152228906	23.933525255168803	20.89330890691791	26.877780685684378
17	26.546279333507805	24.116723370845328	21.813661345197595	27.523335950449273
18	26.12317892349298	21.896536683241735	24.129808950536507	27.85047544272878
19	27.50588851086103	23.597662043095173	22.738375643374333	26.158073802669456
20	27.248538776934485	21.879089243653493	23.061153275756784	27.81121870365524
21	28.62688650440548	22.703480764197852	21.935793422315275	26.733839309081393
22	27.976969379743522	22.328360813050686	22.389426851609525	27.305242955596263
23	27.296519235802148	22.415598010991886	22.546453807903692	27.741428945302278
24	27.863561022419958	22.799441681933178	22.0753729390212	27.261624356625664
25	27.344499694669803	22.232399895315364	22.690395184506674	27.73270522550816
26	27.928988920875863	22.94338305853616	21.813661345197595	27.31396667539039
27	27.322690395184505	22.856145860594957	22.655500305330193	27.16566343889034
28	26.655325830934313	22.52028264852133	23.100410014830324	27.723981505714036
29	25.970513827095875	23.39265462793335	23.061153275756784	27.575678269213995
30	25.75678269213993	23.479891825874553	23.479891825874553	27.283433656110965
31	27.92026520108174	22.472302189653668	22.057925499432958	27.549507109831634
32	26.018494285963534	22.328360813050686	23.026258396580303	28.62688650440548
33	26.105731483904737	22.46357846985955	23.83756433743348	27.593125708802233
34	27.161301578993285	22.53336822821251	23.104771874727383	27.200558318066825
35	26.930123004449097	22.57698682718311	23.475529965977493	27.0173602023903
36	25.93561894791939	23.113495594521506	23.527872284742212	27.42301317281689
37	27.183110878478583	20.81915728866789	24.27811218703655	27.71961964581698
38	24.97164791066911	21.66971996859461	25.44709063944866	27.91154148128762
39	25.726249672860508	21.028526563726775	26.044665445345892	27.200558318066825
40	25.11122742737503	20.95437494547675	26.480851435051907	27.45354619209631
41	24.61397539911018	20.85405216784437	27.38375643374335	27.1482159993021
42	24.339178225595393	20.762453110006106	27.61493500828753	27.283433656110965
43	23.540957864433395	20.640321032888423	28.00314053912588	27.8155805635523
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	5.0
6	3.0
7	1.0
8	0.5
9	0.0
10	2.0
11	4.0
12	4.0
13	4.5
14	5.0
15	12.5
16	20.0
17	21.0
18	22.0
19	22.0
20	25.0
21	28.0
22	32.5
23	37.0
24	55.0
25	73.0
26	73.0
27	87.5
28	102.0
29	143.0
30	184.0
31	229.5
32	275.0
33	275.0
34	339.0
35	403.0
36	456.5
37	510.0
38	666.5
39	823.0
40	823.0
41	926.0
42	1029.0
43	1043.5
44	1058.0
45	1256.0
46	1454.0
47	1454.0
48	1555.0
49	1656.0
50	1818.5
51	1981.0
52	2070.0
53	2159.0
54	2159.0
55	2041.5
56	1924.0
57	1917.0
58	1910.0
59	1771.0
60	1632.0
61	1632.0
62	1551.0
63	1470.0
64	1369.5
65	1269.0
66	1096.0
67	923.0
68	923.0
69	815.0
70	707.0
71	616.5
72	526.0
73	421.5
74	317.0
75	317.0
76	231.5
77	146.0
78	134.0
79	122.0
80	100.0
81	78.0
82	78.0
83	58.0
84	38.0
85	27.5
86	17.0
87	13.0
88	9.0
89	9.0
90	7.5
91	6.0
92	4.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	22926.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.09116287184855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.41336887430798	78.63561022419961
2	4.239286446586016	7.214516269737416
3	1.4045519786754153	3.5854488353834078
4	0.6766454787779372	2.303062025647736
5	0.4100881689563256	1.7447439588240428
6	0.26143120770965755	1.3347291285003926
7	0.16403526758253026	0.9770566169414638
8	0.08201763379126513	0.5583180668236937
9	0.0871437359032192	0.6673645642501963
>10	0.2563051055977035	2.7261624356625664
>50	0.005126102111954071	0.25298787402948614
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	58	0.25298787402948614	TruSeq Adapter, Index 12 (95% over 22bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.008723719794120214	0.0
26	0.0	0.0	0.0	0.01308557969118032	0.0
27	0.0	0.0	0.0	0.017447439588240427	0.0
28	0.0	0.0	0.0	0.05670417866178138	0.0
29	0.0	0.0	0.0	0.12213207711768298	0.0
30	0.0	0.0	0.0	0.23990229433830584	0.0
31	0.0	0.0	0.0	0.4710808688824915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	20	0.0018045281	37.0	35
CCGTCTT	20	0.0018045281	37.0	37
GCCGTCT	30	3.4914506E-4	30.833334	36
TATGCCG	25	0.0053859795	29.6	33
ATGCCGT	25	0.0053859795	29.6	34
CGAGCCC	40	0.0018743054	23.125	5
TCCGAGC	40	0.0018743054	23.125	3
GTCTCTT	45	0.003714181	20.555555	36
CCGAGCC	45	0.003714181	20.555555	4
TCTCTTA	45	0.003714181	20.555555	37
CTCCGAG	45	0.003714181	20.555555	2
TGTCTCT	125	3.45608E-10	19.24	37
CTGTCTC	155	6.0554157E-9	15.5161295	36
>>END_MODULE