##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632348.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4373
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.787102675508805	34.0	31.0	34.0	31.0	34.0
2	32.974845643722844	34.0	33.0	34.0	31.0	34.0
3	33.06059913103133	34.0	33.0	34.0	31.0	34.0
4	36.429224788474734	37.0	37.0	37.0	35.0	37.0
5	36.28470157786417	37.0	37.0	37.0	35.0	37.0
6	36.306654470615136	37.0	37.0	37.0	35.0	37.0
7	36.306197118682825	37.0	37.0	37.0	35.0	37.0
8	36.31442945346444	37.0	37.0	37.0	35.0	37.0
9	38.09490052595472	39.0	39.0	39.0	37.0	39.0
10	38.095815229819344	39.0	38.0	39.0	35.0	39.0
11	38.14040704321976	39.0	39.0	39.0	37.0	39.0
12	38.09055568259776	39.0	38.0	39.0	37.0	39.0
13	38.128744568945805	39.0	39.0	39.0	37.0	39.0
14	39.5145209238509	40.0	39.0	41.0	37.0	41.0
15	39.54081865995884	40.0	39.0	41.0	37.0	41.0
16	39.506288589069285	41.0	39.0	41.0	37.0	41.0
17	39.47564600960439	41.0	39.0	41.0	37.0	41.0
18	39.45277841298879	40.0	39.0	41.0	37.0	41.0
19	39.50285844957695	41.0	39.0	41.0	37.0	41.0
20	39.50445918134004	41.0	39.0	41.0	37.0	41.0
21	39.453693116853415	41.0	39.0	41.0	37.0	41.0
22	39.49028127143837	40.0	39.0	41.0	37.0	41.0
23	39.4548364966842	40.0	39.0	41.0	37.0	41.0
24	39.43814315115481	41.0	39.0	41.0	37.0	41.0
25	39.305053738852045	40.0	39.0	41.0	36.0	41.0
26	39.2341641893437	40.0	39.0	41.0	36.0	41.0
27	39.1822547450263	40.0	39.0	41.0	36.0	41.0
28	39.11136519551795	40.0	39.0	41.0	36.0	41.0
29	39.10564829636405	40.0	39.0	41.0	36.0	41.0
30	39.03407271895724	40.0	39.0	41.0	35.0	41.0
31	39.004116167390805	40.0	39.0	41.0	35.0	41.0
32	38.95655156643037	40.0	38.0	41.0	35.0	41.0
33	38.83901211982621	40.0	38.0	41.0	35.0	41.0
34	38.793734278527324	40.0	38.0	41.0	35.0	41.0
35	38.75051452092385	40.0	38.0	41.0	35.0	41.0
36	38.70729476332038	40.0	38.0	41.0	35.0	41.0
37	38.73656528698834	40.0	38.0	41.0	35.0	41.0
38	38.671621312600045	40.0	38.0	41.0	35.0	41.0
39	38.624056711639604	40.0	38.0	41.0	35.0	41.0
40	38.591356048479305	40.0	38.0	41.0	35.0	41.0
41	38.51703635947862	40.0	38.0	41.0	35.0	41.0
42	38.42579464898239	40.0	38.0	41.0	34.0	41.0
43	37.66956322890464	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	2.0
22	1.0
23	2.0
24	2.0
25	1.0
26	3.0
27	5.0
28	9.0
29	16.0
30	26.0
31	37.0
32	53.0
33	66.0
34	110.0
35	148.0
36	225.0
37	429.0
38	1104.0
39	2134.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01394923393551	17.790990166933454	12.805854104733594	26.38920649439744
2	20.85524811342328	19.757603475874685	32.83786873999542	26.54927967070661
3	21.518408415275555	21.358335238966383	28.172879030413906	28.950377315344156
4	17.92819574662703	15.092613766293162	29.384861651040477	37.59432883603933
5	17.17356505831237	33.706837411388065	32.357649211067915	16.76194831923165
6	37.45712325634576	30.665447061513834	14.863937800137206	17.0134918820032
7	30.391035902126685	28.378687399954266	19.62039789618111	21.609878801737935
8	28.83603933226618	31.32860736336611	19.36885433340956	20.466498970958153
9	26.869426023324948	13.812028355819802	18.271209695860964	41.047335924994286
10	18.477018065401328	26.091927738394695	31.191401783672536	24.23965241253144
11	36.999771324033844	20.032014635261834	19.597530299565516	23.370683741138805
12	21.564143608506747	24.376857992225016	27.761262291333182	26.297736107935055
13	32.929339126457805	18.591356048479305	22.112965927281042	26.366338897781844
14	24.78847473130574	20.37502858449577	21.678481591584724	33.158015092613766
15	27.258175165790078	27.258175165790078	18.797164418019666	26.68648525040018
16	28.378687399954266	23.759432883603935	20.512234164189344	27.34964555225246
17	26.00045735193231	25.017150697461698	23.62222730391036	25.360164646695633
18	25.977589755316714	23.43928653098559	24.51406357191859	26.069060141779097
19	27.53258632517722	24.23965241253144	22.684655842670935	25.5431054196204
20	27.235307569174477	24.285387605762633	23.3935513377544	25.08575348730848
21	27.212439972558883	24.51406357191859	22.250171506974617	26.02332494854791
22	28.035673450720328	22.8675966155957	22.61605305282415	26.48067688085982
23	26.32060370455065	24.331122798993825	22.158701120512234	27.18957237594329
24	27.6469243082552	24.056711639606675	22.18156871712783	26.11479533501029
25	28.950377315344156	22.638920649439743	23.46215412760119	24.94854790761491
26	26.00045735193231	24.879945117768123	23.18774296821404	25.931854562085526
27	27.372513148868055	24.674136748227763	22.227303910359023	25.726046192545166
28	26.02332494854791	23.3935513377544	23.98810884975989	26.5950148639378
29	26.777955636862565	23.34781614452321	24.376857992225016	25.497370226389204
30	25.451635033158016	23.09627258175166	23.62222730391036	27.829865081179967
31	26.52641207409101	24.102446832837867	22.455979876514977	26.91516121655614
32	25.15435627715527	24.125314429453464	24.765607134690143	25.95472215870112
33	25.771781385776354	22.959067002058084	24.491195975302997	26.777955636862565
34	27.46398353533044	22.227303910359023	23.416418934369997	26.892293619940546
35	25.703178595929565	23.78230048021953	24.03384404299108	26.48067688085982
36	26.709352847015776	22.318774296821402	23.98810884975989	26.98376400640293
37	24.651269151612166	21.678481591584724	24.491195975302997	29.179053281500117
38	24.056711639606675	22.021495540818663	27.34964555225246	26.572147267322205
39	25.291561856848844	20.946718499885662	27.303910359021266	26.457809284244227
40	23.736565286988338	19.025840384175623	30.276697919048708	26.96089640978733
41	24.171049622684656	20.306425794648984	27.92133546764235	27.60118911502401
42	21.86142236450949	21.564143608506747	29.224788474731305	27.34964555225246
43	21.907157557740682	20.237823004802195	30.093757146123945	27.761262291333182
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.0
6	0.5
7	0.0
8	0.5
9	1.0
10	2.0
11	3.0
12	3.0
13	2.0
14	1.0
15	2.5
16	4.0
17	5.0
18	6.0
19	6.0
20	5.5
21	5.0
22	11.0
23	17.0
24	18.5
25	20.0
26	20.0
27	20.5
28	21.0
29	31.5
30	42.0
31	61.5
32	81.0
33	81.0
34	85.5
35	90.0
36	113.5
37	137.0
38	150.5
39	164.0
40	164.0
41	188.5
42	213.0
43	223.0
44	233.0
45	251.0
46	269.0
47	269.0
48	292.0
49	315.0
50	345.0
51	375.0
52	390.5
53	406.0
54	406.0
55	398.5
56	391.0
57	373.0
58	355.0
59	339.0
60	323.0
61	323.0
62	290.0
63	257.0
64	223.5
65	190.0
66	173.0
67	156.0
68	156.0
69	135.0
70	114.0
71	96.0
72	78.0
73	56.0
74	34.0
75	34.0
76	28.5
77	23.0
78	22.5
79	22.0
80	18.5
81	15.0
82	15.0
83	11.0
84	7.0
85	4.0
86	1.0
87	1.0
88	1.0
89	1.0
90	1.5
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4373.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.54081865995883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.88846337960747	92.5680310999314
2	2.4174246050741983	4.619254516350332
3	0.47869794159885115	1.372055796935742
4	0.11967448539971279	0.45735193231191396
5	0.023934897079942556	0.11433798307797849
6	0.023934897079942556	0.13720557969357422
7	0.023934897079942556	0.1600731763091699
8	0.0	0.0
9	0.0	0.0
>10	0.023934897079942556	0.5716899153898924
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	25	0.5716899153898924	RNA PCR Primer, Index 17 (95% over 21bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	7	0.1600731763091699	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	6	0.13720557969357422	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	5	0.11433798307797849	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0228675966155957	0.0
18	0.0	0.0	0.0	0.0228675966155957	0.0
19	0.0	0.0	0.0	0.0228675966155957	0.0
20	0.0	0.0	0.0	0.0457351932311914	0.0
21	0.0	0.0	0.0	0.0457351932311914	0.0
22	0.0	0.0	0.0	0.0457351932311914	0.0
23	0.0	0.0	0.0	0.0457351932311914	0.0
24	0.0	0.0	0.0	0.0457351932311914	0.0
25	0.0	0.0	0.0	0.0457351932311914	0.0
26	0.0	0.0	0.0	0.0457351932311914	0.0
27	0.0	0.0	0.0	0.0686027898467871	0.0
28	0.0	0.0	0.0	0.1829407729247656	0.0
29	0.0	0.0	0.0	0.2744111593871484	0.0
30	0.0	0.0	0.0	0.457351932311914	0.0
31	0.0	0.0	0.0	0.8689686713926367	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE