##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632347.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	111700
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.864127126230976	31.0	31.0	34.0	30.0	34.0
2	32.06746642793196	33.0	31.0	34.0	30.0	34.0
3	32.1418800358102	34.0	31.0	34.0	30.0	34.0
4	35.810358102059084	37.0	35.0	37.0	35.0	37.0
5	35.42268576544315	37.0	35.0	37.0	33.0	37.0
6	35.408343777976725	37.0	35.0	37.0	33.0	37.0
7	35.39750223813787	37.0	35.0	37.0	33.0	37.0
8	35.376723366159354	37.0	35.0	37.0	33.0	37.0
9	37.03964189794091	39.0	37.0	39.0	33.0	39.0
10	36.89628469113698	39.0	37.0	39.0	33.0	39.0
11	36.98406445837063	39.0	37.0	39.0	33.0	39.0
12	36.93515666965085	39.0	37.0	39.0	33.0	39.0
13	37.02097582811101	39.0	37.0	39.0	33.0	39.0
14	38.13516562220233	40.0	38.0	41.0	33.0	41.0
15	38.097842435094	40.0	38.0	41.0	33.0	41.0
16	38.08794091316025	40.0	38.0	41.0	33.0	41.0
17	38.05692927484333	40.0	37.0	41.0	33.0	41.0
18	38.0177260519248	40.0	37.0	41.0	33.0	41.0
19	38.065595344673234	40.0	37.0	41.0	33.0	41.0
20	38.01108325872874	40.0	37.0	41.0	33.0	41.0
21	37.98444046553268	40.0	37.0	41.0	33.0	41.0
22	37.93851387645479	40.0	37.0	41.0	33.0	41.0
23	37.88300805729633	39.0	37.0	40.0	33.0	41.0
24	37.832068039391224	39.0	37.0	41.0	33.0	41.0
25	37.74717994628469	39.0	37.0	40.0	33.0	41.0
26	37.60357206803939	39.0	37.0	40.0	33.0	41.0
27	37.50973142345568	39.0	37.0	40.0	32.0	41.0
28	37.4243509400179	39.0	37.0	40.0	32.0	41.0
29	37.33052820053715	39.0	36.0	40.0	32.0	41.0
30	37.22611459265891	39.0	36.0	40.0	32.0	41.0
31	37.14641002685765	39.0	36.0	40.0	31.0	41.0
32	37.05703670546106	39.0	36.0	40.0	31.0	41.0
33	36.965120859444944	39.0	36.0	40.0	31.0	41.0
34	36.859668755595344	39.0	36.0	40.0	31.0	41.0
35	36.828576544315126	39.0	36.0	40.0	31.0	41.0
36	36.77368845120859	39.0	35.0	40.0	30.0	41.0
37	36.701915846016114	39.0	35.0	40.0	30.0	41.0
38	36.676911369740374	39.0	35.0	40.0	30.0	41.0
39	36.61575649059982	39.0	35.0	40.0	30.0	41.0
40	36.548997314234555	38.0	35.0	40.0	30.0	41.0
41	36.47753804834378	38.0	35.0	40.0	30.0	41.0
42	36.440895255147716	38.0	35.0	40.0	30.0	41.0
43	35.502166517457475	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	0.0
17	2.0
18	8.0
19	7.0
20	20.0
21	40.0
22	67.0
23	127.0
24	183.0
25	301.0
26	492.0
27	709.0
28	966.0
29	1336.0
30	1811.0
31	2453.0
32	3188.0
33	4271.0
34	5956.0
35	8529.0
36	13275.0
37	21117.0
38	30935.0
39	15905.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.946284691136974	19.30796777081468	13.073410922112801	26.672336615935542
2	20.20769919427037	20.032229185317814	33.241718889883614	26.5183527305282
3	21.493285586392123	21.426141450313338	28.35989256938227	28.72068039391226
4	16.62667860340197	15.887197851387647	32.184422560429724	35.301700984780666
5	16.973142345568487	33.90152193375112	32.14861235452104	16.976723366159355
6	35.71083258728738	32.940017905102955	15.125335720680393	16.223813786929277
7	30.35273052820054	27.933751119068933	20.55595344673232	21.15756490599821
8	27.22918531781558	32.23097582811101	19.501342882721577	21.038495971351836
9	26.803939122649957	14.333930170098478	19.723366159355415	39.13876454789615
10	18.933751119068933	25.435989256938228	31.45120859444942	24.17905102954342
11	37.091316025067144	20.84243509400179	20.18173679498657	21.884512085944493
12	23.017009847806623	24.287376902417186	27.07967770814682	25.615935541629366
13	31.28379588182632	18.65085049239033	23.393017009847807	26.672336615935542
14	23.68128916741271	20.247985675917636	23.54610564010743	32.52461951656222
15	27.111011638316924	26.17636526410027	20.602506714413607	26.110116383169203
16	26.108325872873767	24.519247985675918	22.802148612354518	26.570277529095794
17	24.89435989256938	25.257833482542523	24.131602506714415	25.71620411817368
18	25.134288272157562	23.92032229185318	24.86750223813787	26.077887197851386
19	26.128021486123544	24.390331244404656	24.53446732318711	24.94717994628469
20	25.828111011638317	23.962399283795882	24.734109221128023	25.47538048343778
21	26.892569382273944	23.76902417188899	23.99373321396598	25.344673231871084
22	26.806624888093104	23.982990152193377	23.724261414503133	25.486123545210386
23	26.084153983885404	24.032229185317817	23.80841539838854	26.075201432408235
24	25.99015219337511	24.279319606087736	23.623097582811102	26.107430617726052
25	25.970456580125333	23.641002685765443	24.205908683974933	26.182632050134284
26	26.034914950760967	24.184422560429724	24.265890778871977	25.514771709937335
27	26.296329453894362	23.90689346463742	23.856759176365262	25.940017905102952
28	25.3034914950761	23.846016114592658	24.896150402864816	25.954341987466428
29	25.37332139659803	23.949865711727842	24.752014324082364	25.92479856759176
30	25.105640107430617	24.174574753804833	24.946284691136974	25.773500447627573
31	25.799462846911368	23.745747538048345	24.53446732318711	25.920322291853175
32	24.924798567591765	23.939122649955237	24.53357206803939	26.60250671441361
33	25.338406445837062	23.988361683079678	24.5496866606983	26.123545210384957
34	25.857654431512984	23.178155774395705	24.63294538943599	26.331244404655322
35	25.77887197851388	23.12712623097583	25.17009847806625	25.923903312444047
36	25.25604297224709	22.992837958818264	24.900626678603402	26.850492390331244
37	24.887197851387647	21.91316025067144	26.241718889883618	26.957923008057293
38	24.56759176365264	21.75649059982095	27.122649955237243	26.553267681289167
39	23.93285586392122	21.68128916741271	27.646374216651747	26.739480752014323
40	23.83616830796777	21.43509400179051	28.772605192479855	25.956132497761864
41	22.717994628469114	21.658907788719787	29.066248880931067	26.556848701880035
42	22.79946284691137	21.952551477170996	29.076096687555953	26.171888988361687
43	22.01880035810206	21.70008952551477	29.14234556848702	27.138764547896148
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	2.5
2	2.0
3	9.0
4	16.0
5	16.0
6	19.5
7	23.0
8	24.0
9	25.0
10	41.0
11	57.0
12	57.0
13	105.5
14	154.0
15	209.5
16	265.0
17	265.5
18	266.0
19	266.0
20	343.0
21	420.0
22	445.5
23	471.0
24	570.5
25	670.0
26	670.0
27	804.0
28	938.0
29	1234.0
30	1530.0
31	1915.0
32	2300.0
33	2300.0
34	2871.5
35	3443.0
36	3981.5
37	4520.0
38	4917.0
39	5314.0
40	5314.0
41	5695.5
42	6077.0
43	6535.0
44	6993.0
45	7249.0
46	7505.0
47	7505.0
48	7787.5
49	8070.0
50	8202.5
51	8335.0
52	8471.0
53	8607.0
54	8607.0
55	8458.5
56	8310.0
57	7945.5
58	7581.0
59	7088.5
60	6596.0
61	6596.0
62	6304.5
63	6013.0
64	5316.0
65	4619.0
66	4156.0
67	3693.0
68	3693.0
69	3182.5
70	2672.0
71	2364.0
72	2056.0
73	1891.5
74	1727.0
75	1727.0
76	1523.0
77	1319.0
78	941.5
79	564.0
80	401.5
81	239.0
82	239.0
83	193.5
84	148.0
85	117.0
86	86.0
87	66.0
88	46.0
89	46.0
90	33.0
91	20.0
92	13.5
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	111700.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.73321396598031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.33321962590256	71.90957923008058
2	4.600602649383137	7.244404655326768
3	1.421342884757519	3.357206803939123
4	0.7914037182329865	2.4923903312444047
5	0.41048382511797143	1.6159355416293641
6	0.32747740064813236	1.5470008952551477
7	0.1864801864801865	1.02775290957923
8	0.1591904030928421	1.0026857654431514
9	0.12394109955085565	0.8782452999104745
>10	0.6299391665245324	7.992837958818264
>50	0.012507817385866166	0.6195165622202328
>100	0.0034112229234180454	0.3124440465532677
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	131	0.11727842435094002	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	113	0.10116383169203223	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	8.952551477170994E-4	0.0	0.0	0.0	0.0
11	8.952551477170994E-4	0.0	0.0	0.0	0.0
12	0.0026857654431512983	0.0	0.0	0.0	0.0
13	0.0035810205908683975	0.0	0.0	0.0	0.0
14	0.0035810205908683975	0.0	0.0	0.0	0.0
15	0.0035810205908683975	0.0	0.0	0.0	0.0
16	0.004476275738585497	0.0	0.0	8.952551477170994E-4	0.0
17	0.004476275738585497	0.0	0.0	0.004476275738585497	0.0
18	0.004476275738585497	0.0	0.0	0.004476275738585497	0.0
19	0.004476275738585497	0.0	0.0	0.004476275738585497	0.0
20	0.004476275738585497	0.0	0.0	0.005371530886302597	0.0
21	0.005371530886302597	0.0	0.0	0.005371530886302597	0.0
22	0.005371530886302597	0.0	0.0	0.006266786034019696	0.0
23	0.005371530886302597	0.0	0.0	0.008952551477170993	0.0
24	0.005371530886302597	0.0	0.0	0.01432408236347359	0.0
25	0.005371530886302597	0.0	0.0	0.018800358102059087	0.0
26	0.005371530886302597	0.0	0.0	0.025962399283795883	0.0
27	0.005371530886302597	0.0	0.0	0.06803939122649955	0.0
28	0.006266786034019696	0.0	0.0	0.170993733213966	0.0
29	0.006266786034019696	0.0	0.0	0.3106535362578335	0.0
30	0.006266786034019696	0.0	0.0	0.4619516562220233	0.0
31	0.006266786034019696	0.0	0.0	0.8334825425246195	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGTGG	25	0.0054753874	29.6	8
ACTAGGT	25	0.0054753874	29.6	28
CCTGACG	25	0.0054753874	29.6	8
TTGGTTC	40	0.0019203511	23.125002	5
CCTTGCT	40	0.0019203511	23.125002	9
TCAGAAG	45	0.0038045468	20.555555	18
GTTCACA	45	0.0038045468	20.555555	8
GACAGGC	55	5.103254E-4	20.181818	7
GGTATCA	115	2.9649527E-9	19.304348	1
AGTCCTT	50	0.0069961613	18.5	6
TATACAC	60	9.165848E-4	18.5	37
TGTTGGT	65	0.0015678944	17.076923	3
TCTTATA	185	1.8189894E-12	17.0	37
GGTGTTG	70	0.0025729001	15.857143	1
GCTGGGT	85	5.310514E-4	15.235293	24
GTTCCCC	75	0.0040736417	14.8	17
ACGAGGC	75	0.0040736417	14.8	17
GAGGCTT	75	0.0040736417	14.8	19
TAGAAGA	90	8.1918767E-4	14.388888	4
GTGTTGG	80	0.0062518013	13.875001	2
>>END_MODULE