FastQCFastQC Report
Fri 10 Feb 2017
ERR1632346.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632346.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences391853
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12930.3299706777796776No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11190.2855662710251038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9030.23044355919183981No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.12147412422515586No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTC358.858925E-426.4285717
ACCGTCG551.8985127E-523.5454548
ACCCGTC400.001928928723.12514
GGTATCA3200.021.968751
GTATTAG1059.767973E-1021.1428571
GTAGCCT450.003821377420.5555554
ACTGTCC555.1347836E-420.1818188
TACCGTC555.1347836E-420.1818187
ATACCGT609.222012E-418.56
TAGAACT500.007026812518.4999984
ATTTGCG752.0628888E-417.26666616
GTTATTC752.0628888E-417.2666663
CCGTCGT650.001577422517.0769239
GTATCAA5800.015.9482761
TAGAACC700.002588407815.8571424
CGACCCG700.002588407815.8571425
CGTCGTA700.002588407815.85714210
GTCTTGA700.002588407815.8571421
CGGGAGT957.040713E-515.5789484
CAAATCG855.352954E-415.23529413
Produced by FastQC (version 0.11.5)