##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632346.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	391853
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83135002156421	34.0	31.0	34.0	31.0	34.0
2	32.990948136163304	34.0	33.0	34.0	31.0	34.0
3	33.075589570578764	34.0	33.0	34.0	31.0	34.0
4	36.44362043929739	37.0	37.0	37.0	35.0	37.0
5	36.31412800208241	37.0	37.0	37.0	35.0	37.0
6	36.34326903200945	37.0	37.0	37.0	35.0	37.0
7	36.326004904900564	37.0	37.0	37.0	35.0	37.0
8	36.32536691055064	37.0	37.0	37.0	35.0	37.0
9	38.11831732818174	39.0	39.0	39.0	37.0	39.0
10	38.100149801073364	39.0	38.0	39.0	37.0	39.0
11	38.15005627110166	39.0	39.0	39.0	37.0	39.0
12	38.1285099259161	39.0	38.0	39.0	37.0	39.0
13	38.14182869596507	39.0	39.0	39.0	37.0	39.0
14	39.55785970759443	41.0	39.0	41.0	37.0	41.0
15	39.55673173358377	41.0	39.0	41.0	37.0	41.0
16	39.542999543196046	41.0	39.0	41.0	37.0	41.0
17	39.54053433302795	41.0	39.0	41.0	37.0	41.0
18	39.52671792738604	41.0	39.0	41.0	37.0	41.0
19	39.549629580480435	41.0	39.0	41.0	37.0	41.0
20	39.51610170140333	41.0	39.0	41.0	37.0	41.0
21	39.49656121045392	41.0	39.0	41.0	37.0	41.0
22	39.44833649353201	41.0	39.0	41.0	37.0	41.0
23	39.39255792350703	41.0	39.0	41.0	37.0	41.0
24	39.336062247832736	41.0	39.0	41.0	36.0	41.0
25	39.29811434389937	40.0	39.0	41.0	36.0	41.0
26	39.20923917897783	40.0	39.0	41.0	36.0	41.0
27	39.125917116878014	40.0	39.0	41.0	36.0	41.0
28	39.05612308697394	40.0	39.0	41.0	35.0	41.0
29	39.00198543841696	40.0	39.0	41.0	35.0	41.0
30	38.932916170094394	40.0	38.0	41.0	35.0	41.0
31	38.85584134866902	40.0	38.0	41.0	35.0	41.0
32	38.769913717644116	40.0	38.0	41.0	35.0	41.0
33	38.68897775441301	40.0	38.0	41.0	35.0	41.0
34	38.609833279316476	40.0	38.0	41.0	35.0	41.0
35	38.5761267618214	40.0	38.0	41.0	35.0	41.0
36	38.53717337879256	40.0	38.0	41.0	35.0	41.0
37	38.46632283024501	40.0	38.0	41.0	35.0	41.0
38	38.417791876035146	40.0	38.0	41.0	35.0	41.0
39	38.35673071279281	40.0	38.0	41.0	34.0	41.0
40	38.27304116594743	40.0	38.0	41.0	34.0	41.0
41	38.20685563208652	40.0	38.0	41.0	34.0	41.0
42	38.13604846715477	40.0	38.0	41.0	34.0	41.0
43	37.28591589192886	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	7.0
19	13.0
20	20.0
21	48.0
22	90.0
23	140.0
24	217.0
25	388.0
26	592.0
27	853.0
28	1248.0
29	1725.0
30	2438.0
31	3287.0
32	4605.0
33	6270.0
34	9442.0
35	13819.0
36	22118.0
37	41382.0
38	94167.0
39	188982.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.371756755722174	18.638111740882422	12.503923665252021	26.486207838143383
2	19.26334620380602	20.411991231405654	33.69273681712275	26.63192574766558
3	20.12693535586049	21.97635337741449	29.40235241276703	28.49435885395799
4	15.0834624208568	15.685473889443236	33.63914529172929	35.591918397970666
5	16.15197535810623	34.59052246633304	32.904175800619115	16.353326374941624
6	34.80003981084743	33.7552602634151	15.814603945867455	15.630095979870001
7	29.493968401415838	29.46742783645908	21.15972060951428	19.8788831526108
8	27.576667781030128	31.969131281373368	20.0769165988266	20.377284338769897
9	26.81413693400331	14.1685785230686	19.91920439552587	39.098080147402214
10	17.918709311910334	25.804829872426648	32.227901789701754	24.048559025961268
11	36.572132917190885	21.231941569925457	21.156913434374623	21.039012078509035
12	22.28999139983616	24.942516709072024	28.461693543241985	24.30579834784983
13	30.732698231224465	19.267939763125458	23.997264280227533	26.002097725422544
14	23.237285410600403	20.4370006099226	24.60335891265347	31.722355066823525
15	25.88164439215726	26.994816933901234	21.76964320803975	25.353895465901754
16	25.433261963032056	25.481239138146194	23.814032302929924	25.271466595891827
17	24.26241473205513	25.732864109755443	25.10737444909188	24.897346709097544
18	24.40711185061745	24.44181874325321	26.179460154700866	24.971609251428468
19	24.856004675222596	24.858811850362255	26.006180889262044	24.279002585153105
20	25.069094788096557	24.524502811003106	25.844130324381847	24.56227207651849
21	25.82192812100456	24.616629195131846	25.265086652392604	24.296356031470985
22	25.72316659563663	24.43492840427405	25.128811059249262	24.71309394084006
23	25.070370776796402	24.30171518401033	25.423054053433304	25.204859985759963
24	24.755711963414853	24.887649194978728	25.233186934896505	25.123451906709914
25	24.984113940686942	24.356072302623687	25.544017782178518	25.115795974510853
26	24.718963488859345	25.02009682202254	25.48966066356516	24.77127902555295
27	24.910361793835953	24.458406596351182	25.516966821741825	25.11426478807104
28	24.4566202121714	24.61816038157166	25.98091631300513	24.944303093251808
29	24.225921455239593	24.858811850362255	25.99035862938398	24.924908065014176
30	24.147830946809133	25.175257047923584	25.897466652035327	24.779445353231953
31	24.741676087716566	24.925928855974053	25.43377235851199	24.898622697797386
32	23.89569557971995	24.840948008564435	25.681569364021712	25.581787047693904
33	23.837765692747027	24.429058856254766	26.362181736518544	25.370993714479667
34	24.674559082104768	24.14247179426979	26.059006821435588	25.123962302189852
35	24.49770704830638	24.07969315023746	26.611254730728106	24.811345070728052
36	24.054428573980548	24.21418235920103	26.360140154598792	25.371248912219635
37	23.962557387591776	23.52387247258538	26.96776597346454	25.545804166358305
38	23.39168004328154	23.078807614079768	27.956657215843695	25.572855126794998
39	23.437360438735954	22.726634732922804	28.226146029250764	25.609858799090475
40	23.14337264229188	22.71132286852468	28.879197045830963	25.26610744335248
41	22.242779817941933	22.67687117362889	29.6595917346556	25.42075727377358
42	22.173621230410383	22.85959275544656	29.604979418302275	25.361806595840786
43	21.56038106126532	22.337968574950303	30.021206932191408	26.08044343159297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.5
2	105.0
3	186.5
4	268.0
5	268.0
6	316.0
7	364.0
8	352.0
9	340.0
10	519.5
11	699.0
12	699.0
13	1093.0
14	1487.0
15	2152.0
16	2817.0
17	2686.0
18	2555.0
19	2555.0
20	2934.0
21	3313.0
22	3057.0
23	2801.0
24	3386.5
25	3972.0
26	3972.0
27	4644.5
28	5317.0
29	6302.0
30	7287.0
31	8368.5
32	9450.0
33	9450.0
34	11030.5
35	12611.0
36	14147.5
37	15684.0
38	17279.0
39	18874.0
40	18874.0
41	20252.5
42	21631.0
43	22715.5
44	23800.0
45	24823.5
46	25847.0
47	25847.0
48	26711.0
49	27575.0
50	27991.5
51	28408.0
52	28359.5
53	28311.0
54	28311.0
55	27751.5
56	27192.0
57	26293.5
58	25395.0
59	24359.5
60	23324.0
61	23324.0
62	21487.5
63	19651.0
64	17571.0
65	15491.0
66	13692.0
67	11893.0
68	11893.0
69	10315.0
70	8737.0
71	7509.5
72	6282.0
73	5016.5
74	3751.0
75	3751.0
76	3026.5
77	2302.0
78	1918.5
79	1535.0
80	1323.0
81	1111.0
82	1111.0
83	870.0
84	629.0
85	548.5
86	468.0
87	391.5
88	315.0
89	315.0
90	216.0
91	117.0
92	73.5
93	30.0
94	16.5
95	3.0
96	3.0
97	4.5
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	391853.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.64981596632873
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.37554639444053	70.039188149566
2	5.00321112455032	7.669904238749414
3	1.3522725850751802	3.1095433434697255
4	0.635723005299865	1.9491220544718433
5	0.3675952240573682	1.4088053137049328
6	0.23850733407780655	1.0968925958210136
7	0.17029121323256818	0.9136953108471438
8	0.12967774820685224	0.7951820427986512
9	0.10319109585645503	0.7118620656084961
>10	0.5693564859402817	8.013404007840014
>50	0.040637745682863075	2.1043283396002863
>100	0.01299075475280543	1.341694420616186
>500	3.330962757129597E-4	0.2305518671391848
>1k	6.661925514259194E-4	0.6158262497671249
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1293	0.3299706777796776	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1119	0.2855662710251038	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	903	0.23044355919183981	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.12147412422515586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.551977399688148E-4	0.0
13	0.0	0.0	0.0	2.551977399688148E-4	0.0
14	0.0	0.0	0.0	5.103954799376296E-4	0.0
15	0.0	0.0	0.0	0.0010207909598752593	0.0
16	0.0	0.0	0.0	0.0010207909598752593	0.0
17	0.0	0.0	0.0	0.001531186439812889	0.0
18	0.0	0.0	0.0	0.0020415819197505186	0.0
19	0.0	0.0	0.0	0.0020415819197505186	0.0
20	0.0	0.0	0.0	0.0022967796597193335	0.0
21	0.0	0.0	0.0	0.0025519773996881484	0.0
22	0.0	0.0	0.0	0.003317570619594593	0.0
23	0.0	0.0	0.0	0.004338361579469852	0.0
24	0.0	0.0	0.0	0.005103954799376297	0.0
25	0.0	0.0	0.0	0.008676723158939704	0.0
26	0.0	0.0	0.0	0.01531186439812889	0.0
27	0.0	0.0	0.0	0.044149209014604965	0.0
28	0.0	0.0	0.0	0.14163474568269224	0.0
29	0.0	0.0	0.0	0.2526457625691267	0.0
30	0.0	0.0	0.0	0.40168124271091454	0.0
31	0.0	0.0	0.0	0.7888162142436067	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTC	35	8.858925E-4	26.42857	17
ACCGTCG	55	1.8985127E-5	23.545454	8
ACCCGTC	40	0.0019289287	23.125	14
GGTATCA	320	0.0	21.96875	1
GTATTAG	105	9.767973E-10	21.142857	1
GTAGCCT	45	0.0038213774	20.555555	4
ACTGTCC	55	5.1347836E-4	20.181818	8
TACCGTC	55	5.1347836E-4	20.181818	7
ATACCGT	60	9.222012E-4	18.5	6
TAGAACT	50	0.0070268125	18.499998	4
ATTTGCG	75	2.0628888E-4	17.266666	16
GTTATTC	75	2.0628888E-4	17.266666	3
CCGTCGT	65	0.0015774225	17.076923	9
GTATCAA	580	0.0	15.948276	1
TAGAACC	70	0.0025884078	15.857142	4
CGACCCG	70	0.0025884078	15.857142	5
CGTCGTA	70	0.0025884078	15.857142	10
GTCTTGA	70	0.0025884078	15.857142	1
CGGGAGT	95	7.040713E-5	15.578948	4
CAAATCG	85	5.352954E-4	15.235294	13
>>END_MODULE