FastQCFastQC Report
Fri 10 Feb 2017
ERR1632345.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632345.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7326
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT4786.5247065247065255No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT4245.787605787605788No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC3164.313404313404313No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC2493.3988533988533987No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG1802.457002457002457No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC1051.4332514332514332Illumina PCR Primer Index 7 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG670.9145509145509146No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT240.32760032760032765RNA PCR Primer, Index 41 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230.313950313950314No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT170.23205023205023204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150.20475020475020475No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA140.19110019110019108No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT100.1365001365001365RNA PCR Primer, Index 41 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.10920010920010921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC354.6807327E-737.03
TTATACA354.6807327E-737.02
CTTATAC401.3316676E-632.3751
CCGTCTT451.1559407E-424.66666637
GCCGTCT451.1559407E-424.66666636
GGTATCA400.00175445523.1251
TACACAT603.1169337E-521.5833345
ATACACA603.1169337E-521.5833344
GTATCAA450.003478846720.5555552
TGCCGTC852.173879E-517.41176437
ATGCCGT903.5542438E-516.44444536
ACACATC802.8435446E-416.18756
TATGCCG955.6516787E-515.57894835
CACATCT854.5149226E-415.2352947
ACATCTC950.001050533413.6315798
CGTATGC1152.8797513E-412.86956533
GTATGCC1204.1286694E-412.33333434
ATCTCCG1357.605806E-512.3333342
CATCTCC1401.06944084E-411.8928571
TCTCGTA1400.00150923810.57142830