Basic Statistics
Measure | Value |
---|---|
Filename | ERR1632345.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 7326 |
Sequences flagged as poor quality | 0 |
Sequence length | 43 |
%GC | 53 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT | 478 | 6.5247065247065255 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT | 424 | 5.787605787605788 | No Hit |
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC | 316 | 4.313404313404313 | No Hit |
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC | 249 | 3.3988533988533987 | No Hit |
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG | 180 | 2.457002457002457 | No Hit |
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC | 105 | 1.4332514332514332 | Illumina PCR Primer Index 7 (95% over 21bp) |
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG | 67 | 0.9145509145509146 | No Hit |
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT | 24 | 0.32760032760032765 | RNA PCR Primer, Index 41 (95% over 21bp) |
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT | 23 | 0.313950313950314 | No Hit |
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT | 17 | 0.23205023205023204 | No Hit |
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT | 15 | 0.20475020475020475 | No Hit |
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA | 14 | 0.19110019110019108 | No Hit |
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT | 10 | 0.1365001365001365 | RNA PCR Primer, Index 41 (95% over 23bp) |
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 8 | 0.10920010920010921 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATACAC | 35 | 4.6807327E-7 | 37.0 | 3 |
TTATACA | 35 | 4.6807327E-7 | 37.0 | 2 |
CTTATAC | 40 | 1.3316676E-6 | 32.375 | 1 |
CCGTCTT | 45 | 1.1559407E-4 | 24.666666 | 37 |
GCCGTCT | 45 | 1.1559407E-4 | 24.666666 | 36 |
GGTATCA | 40 | 0.001754455 | 23.125 | 1 |
TACACAT | 60 | 3.1169337E-5 | 21.583334 | 5 |
ATACACA | 60 | 3.1169337E-5 | 21.583334 | 4 |
GTATCAA | 45 | 0.0034788467 | 20.555555 | 2 |
TGCCGTC | 85 | 2.173879E-5 | 17.411764 | 37 |
ATGCCGT | 90 | 3.5542438E-5 | 16.444445 | 36 |
ACACATC | 80 | 2.8435446E-4 | 16.1875 | 6 |
TATGCCG | 95 | 5.6516787E-5 | 15.578948 | 35 |
CACATCT | 85 | 4.5149226E-4 | 15.235294 | 7 |
ACATCTC | 95 | 0.0010505334 | 13.631579 | 8 |
CGTATGC | 115 | 2.8797513E-4 | 12.869565 | 33 |
GTATGCC | 120 | 4.1286694E-4 | 12.333334 | 34 |
ATCTCCG | 135 | 7.605806E-5 | 12.333334 | 2 |
CATCTCC | 140 | 1.06944084E-4 | 11.892857 | 1 |
TCTCGTA | 140 | 0.001509238 | 10.571428 | 30 |