##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632345.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7326
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78487578487579	34.0	31.0	34.0	31.0	34.0
2	32.987168987168985	34.0	31.0	34.0	31.0	34.0
3	33.04982254982255	34.0	33.0	34.0	31.0	34.0
4	36.43857493857494	37.0	37.0	37.0	35.0	37.0
5	36.30576030576031	37.0	37.0	37.0	35.0	37.0
6	36.336745836745834	37.0	37.0	37.0	35.0	37.0
7	36.2971607971608	37.0	37.0	37.0	35.0	37.0
8	36.323232323232325	37.0	37.0	37.0	35.0	37.0
9	38.12080262080262	39.0	39.0	39.0	37.0	39.0
10	38.05965055965056	39.0	38.0	39.0	37.0	39.0
11	38.16311766311766	39.0	39.0	39.0	37.0	39.0
12	38.05705705705706	39.0	38.0	39.0	37.0	39.0
13	38.21607971607972	39.0	39.0	39.0	37.0	39.0
14	39.473109473109474	40.0	39.0	41.0	37.0	41.0
15	39.48812448812449	40.0	39.0	41.0	37.0	41.0
16	39.486349986349985	40.0	39.0	41.0	37.0	41.0
17	39.32855582855583	40.0	39.0	41.0	37.0	41.0
18	39.544089544089545	40.0	39.0	41.0	37.0	41.0
19	39.5004095004095	40.0	39.0	41.0	37.0	41.0
20	39.49235599235599	40.0	39.0	41.0	37.0	41.0
21	39.496996996997	40.0	39.0	41.0	37.0	41.0
22	39.202293202293205	40.0	39.0	41.0	36.0	41.0
23	39.42355992355992	40.0	39.0	41.0	37.0	41.0
24	39.1987441987442	40.0	39.0	41.0	36.0	41.0
25	39.070570570570574	40.0	39.0	41.0	36.0	41.0
26	39.09118209118209	40.0	39.0	41.0	36.0	41.0
27	38.71444171444171	40.0	38.0	41.0	35.0	41.0
28	38.971471471471475	40.0	39.0	41.0	35.0	41.0
29	38.78910728910729	40.0	38.0	41.0	35.0	41.0
30	38.807807807807805	40.0	38.0	41.0	35.0	41.0
31	38.78924378924379	40.0	38.0	41.0	35.0	41.0
32	38.34630084630085	40.0	38.0	41.0	35.0	41.0
33	38.37182637182637	40.0	38.0	41.0	35.0	41.0
34	38.26849576849577	40.0	38.0	41.0	34.0	41.0
35	38.23068523068523	40.0	38.0	41.0	34.0	41.0
36	38.32200382200382	40.0	38.0	41.0	34.0	41.0
37	38.22031122031122	40.0	38.0	41.0	34.0	41.0
38	38.27272727272727	40.0	38.0	41.0	34.0	41.0
39	38.09336609336609	40.0	38.0	41.0	34.0	41.0
40	37.941850941850944	40.0	37.0	41.0	33.0	41.0
41	37.986349986349985	40.0	37.0	41.0	33.0	41.0
42	37.753890253890255	40.0	37.0	41.0	33.0	41.0
43	36.895850395850395	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	1.0
23	5.0
24	4.0
25	7.0
26	14.0
27	16.0
28	20.0
29	37.0
30	48.0
31	54.0
32	102.0
33	117.0
34	170.0
35	279.0
36	456.0
37	913.0
38	2116.0
39	2966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.616980616980616	19.724269724269725	17.73136773136773	31.927381927381926
2	15.793065793065795	19.956319956319955	34.83483483483483	29.415779415779415
3	14.632814632814634	23.314223314223316	40.62244062244062	21.43052143052143
4	10.988260988260988	20.242970242970244	24.351624351624352	44.417144417144414
5	13.458913458913461	31.627081627081626	34.91673491673492	19.99726999727
6	31.435981435981436	31.98198198198198	15.11056511056511	21.47147147147147
7	22.235872235872236	31.476931476931476	15.6975156975157	30.58968058968059
8	33.169533169533175	29.38847938847939	18.987168987168985	18.454818454818454
9	19.383019383019384	13.786513786513787	13.90936390936391	52.92110292110293
10	19.956319956319955	24.542724542724542	23.955773955773957	31.545181545181546
11	32.159432159432164	17.103467103467104	19.66966966966967	31.067431067431066
12	17.062517062517063	30.6988806988807	19.314769314769315	32.923832923832926
13	33.53808353808354	13.008463008463009	22.167622167622167	31.285831285831282
14	24.297024297024297	23.955773955773957	17.185367185367184	34.561834561834566
15	27.136227136227138	24.965874965874963	13.677313677313677	34.22058422058422
16	36.21348621348621	19.833469833469834	16.707616707616708	27.245427245427244
17	18.48211848211848	39.653289653289654	16.612066612066613	25.252525252525253
18	30.125580125580125	16.502866502866503	19.164619164619165	34.20693420693421
19	23.25962325962326	26.50832650832651	24.556374556374553	25.675675675675674
20	23.054873054873052	19.915369915369915	18.91891891891892	38.11083811083812
21	34.79388479388479	18.031668031668033	20.898170898170896	26.276276276276278
22	22.836472836472836	34.68468468468468	16.63936663936664	25.83947583947584
23	28.97897897897898	16.844116844116844	17.4993174993175	36.677586677586675
24	32.705432705432706	23.955773955773957	21.94922194922195	21.38957138957139
25	20.93912093912094	22.549822549822547	23.887523887523887	32.623532623532626
26	33.14223314223314	19.505869505869505	19.75156975156975	27.600327600327603
27	20.67977067977068	31.46328146328146	23.45072345072345	24.406224406224407
28	21.812721812721815	17.64946764946765	25.088725088725088	35.44908544908545
29	22.686322686322686	24.242424242424242	26.35817635817636	26.71307671307671
30	18.823368823368824	17.977067977067975	33.005733005733006	30.193830193830195
31	28.992628992628994	19.123669123669124	17.895167895167894	33.98853398853399
32	24.761124761124762	26.822276822276827	28.255528255528255	20.16107016107016
33	18.44116844116844	18.413868413868414	29.4021294021294	33.742833742833746
34	28.91072891072891	25.23887523887524	22.24952224952225	23.6008736008736
35	19.574119574119575	16.885066885066884	42.61534261534262	20.925470925470925
36	25.457275457275458	22.044772044772046	19.79251979251979	32.705432705432706
37	20.857220857220856	23.232323232323232	27.32732732732733	28.58312858312858
38	22.945672945672946	17.39011739011739	29.688779688779686	29.975429975429975
39	27.586677586677588	18.63226863226863	23.996723996723997	29.78432978432978
40	18.604968604968604	15.165165165165165	39.80343980343981	26.426426426426424
41	24.37892437892438	18.08626808626809	24.57002457002457	32.96478296478296
42	21.84002184002184	15.52006552006552	37.7013377013377	24.938574938574938
43	18.741468741468744	15.465465465465467	35.27163527163527	30.52143052143052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.0
7	4.0
8	4.0
9	4.0
10	4.5
11	5.0
12	5.0
13	10.0
14	15.0
15	24.5
16	34.0
17	27.5
18	21.0
19	21.0
20	28.5
21	36.0
22	24.5
23	13.0
24	12.5
25	12.0
26	12.0
27	20.5
28	29.0
29	35.0
30	41.0
31	44.0
32	47.0
33	47.0
34	57.0
35	67.0
36	74.0
37	81.0
38	101.0
39	121.0
40	121.0
41	149.0
42	177.0
43	244.0
44	311.0
45	360.5
46	410.0
47	410.0
48	488.5
49	567.0
50	600.0
51	633.0
52	906.0
53	1179.0
54	1179.0
55	849.5
56	520.0
57	858.0
58	1196.0
59	1051.5
60	907.0
61	907.0
62	588.0
63	269.0
64	238.0
65	207.0
66	187.5
67	168.0
68	168.0
69	136.0
70	104.0
71	82.0
72	60.0
73	48.5
74	37.0
75	37.0
76	34.0
77	31.0
78	19.5
79	8.0
80	6.5
81	5.0
82	5.0
83	4.0
84	3.0
85	2.5
86	2.0
87	2.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.65956865956866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.37375745526839	64.79661479661479
2	4.174950298210735	5.733005733005733
3	0.5964214711729622	1.2285012285012284
4	0.3379721669980119	0.9282009282009281
5	0.09940357852882703	0.34125034125034126
6	0.05964214711729623	0.2457002457002457
7	0.07952286282306163	0.38220038220038216
8	0.019880715705765408	0.10920010920010921
9	0.0	0.0
>10	0.11928429423459246	1.405951405951406
>50	0.019880715705765408	0.9145509145509146
>100	0.11928429423459246	23.914823914823916
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	478	6.5247065247065255	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	424	5.787605787605788	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	316	4.313404313404313	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	249	3.3988533988533987	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	180	2.457002457002457	No Hit
CTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTTC	105	1.4332514332514332	Illumina PCR Primer Index 7 (95% over 21bp)
CACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCG	67	0.9145509145509146	No Hit
ACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGT	24	0.32760032760032765	RNA PCR Primer, Index 41 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23	0.313950313950314	No Hit
TATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTAT	17	0.23205023205023204	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15	0.20475020475020475	No Hit
TTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTA	14	0.19110019110019108	No Hit
ATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCT	10	0.1365001365001365	RNA PCR Primer, Index 41 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8	0.10920010920010921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.01365001365001365	0.0
26	0.0	0.0	0.0	0.01365001365001365	0.0
27	0.0	0.0	0.0	0.06825006825006825	0.0
28	0.0	0.0	0.0	0.1365001365001365	0.0
29	0.0	0.0	0.0	0.313950313950314	0.0
30	0.0	0.0	0.0	0.47775047775047774	0.0
31	0.0	0.0	0.0	0.8326508326508326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACAC	35	4.6807327E-7	37.0	3
TTATACA	35	4.6807327E-7	37.0	2
CTTATAC	40	1.3316676E-6	32.375	1
CCGTCTT	45	1.1559407E-4	24.666666	37
GCCGTCT	45	1.1559407E-4	24.666666	36
GGTATCA	40	0.001754455	23.125	1
TACACAT	60	3.1169337E-5	21.583334	5
ATACACA	60	3.1169337E-5	21.583334	4
GTATCAA	45	0.0034788467	20.555555	2
TGCCGTC	85	2.173879E-5	17.411764	37
ATGCCGT	90	3.5542438E-5	16.444445	36
ACACATC	80	2.8435446E-4	16.1875	6
TATGCCG	95	5.6516787E-5	15.578948	35
CACATCT	85	4.5149226E-4	15.235294	7
ACATCTC	95	0.0010505334	13.631579	8
CGTATGC	115	2.8797513E-4	12.869565	33
GTATGCC	120	4.1286694E-4	12.333334	34
ATCTCCG	135	7.605806E-5	12.333334	2
CATCTCC	140	1.06944084E-4	11.892857	1
TCTCGTA	140	0.001509238	10.571428	30
>>END_MODULE