FastQCFastQC Report
Fri 10 Feb 2017
ERR1632344.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632344.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58524
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.6424714646982435No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3020.5160276126033764No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT2570.4391360809240653TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2220.3793315562846012No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT1690.2887704189734126No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC1180.20162668307019344No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.18283097532636183No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC950.16232656687854555No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG920.1572004647665915No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG890.15207436265463742No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC720.12302645068689769No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC700.1196090492789283No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC630.10764814435103548RNA PCR Primer, Index 1 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA200.001827694537.018
CGACATT200.001827694537.028
GACATTG200.001827694537.029
TAAGTTC303.5582457E-430.83333430
ATGGCGT250.00545433229.617
GCACGAA250.00545433229.611
ACGAATG250.00545433229.613
GGTAAGT358.7687536E-426.4285728
TCGGGTA358.7687536E-426.4285725
AAGTTCC358.7687536E-426.4285731
AGTTCCG358.7687536E-426.4285732
GGTATCA2050.025.2682931
CGGGTAA400.001909480723.12526
CGAAATT400.001909480723.12513
GTCCTAA400.001909480723.1251
CTAAGGT400.001909480723.1254
TCCTAAG400.001909480723.1252
ATTCCTT400.001909480723.12517
GTAAGTT400.001909480723.12529
TACACAT400.001909480723.1255
Produced by FastQC (version 0.11.5)