##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632344.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	58524
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70475360535848	34.0	31.0	34.0	31.0	34.0
2	32.8614072858998	34.0	31.0	34.0	31.0	34.0
3	32.90357801927414	34.0	31.0	34.0	31.0	34.0
4	36.331146196432236	37.0	37.0	37.0	35.0	37.0
5	36.21198140933634	37.0	35.0	37.0	35.0	37.0
6	36.2560488004921	37.0	37.0	37.0	35.0	37.0
7	36.236176611304764	37.0	36.0	37.0	35.0	37.0
8	36.240328754015444	37.0	36.0	37.0	35.0	37.0
9	38.04620326703574	39.0	38.0	39.0	35.0	39.0
10	37.99340441528262	39.0	38.0	39.0	35.0	39.0
11	38.06089809309002	39.0	38.0	39.0	35.0	39.0
12	38.010508509329505	39.0	38.0	39.0	35.0	39.0
13	38.05216663249265	39.0	38.0	39.0	35.0	39.0
14	39.34117626956462	40.0	39.0	41.0	37.0	41.0
15	39.37227462237714	40.0	39.0	41.0	37.0	41.0
16	39.336340646572346	40.0	39.0	41.0	36.0	41.0
17	39.347959811359445	40.0	39.0	41.0	37.0	41.0
18	39.39284737885312	40.0	39.0	41.0	37.0	41.0
19	39.409182557583215	40.0	39.0	41.0	37.0	41.0
20	39.40629485339348	40.0	39.0	41.0	37.0	41.0
21	39.383791265122	40.0	39.0	41.0	37.0	41.0
22	39.32120155833504	40.0	39.0	41.0	36.0	41.0
23	39.27055566946893	40.0	39.0	41.0	36.0	41.0
24	39.19222882919828	40.0	39.0	41.0	36.0	41.0
25	39.12500854350352	40.0	39.0	41.0	36.0	41.0
26	39.01302029936436	40.0	39.0	41.0	35.0	41.0
27	38.91031030004785	40.0	38.0	41.0	35.0	41.0
28	38.862312897272915	40.0	38.0	41.0	35.0	41.0
29	38.734860911762695	40.0	38.0	41.0	35.0	41.0
30	38.63913949832547	40.0	38.0	41.0	35.0	41.0
31	38.527407559291916	40.0	38.0	41.0	35.0	41.0
32	38.39939170254938	40.0	38.0	41.0	35.0	41.0
33	38.29341808488825	40.0	38.0	41.0	34.0	41.0
34	38.18698311803704	40.0	38.0	41.0	34.0	41.0
35	38.13291982776297	40.0	37.0	41.0	34.0	41.0
36	38.065238192878134	40.0	37.0	41.0	34.0	41.0
37	37.93532567835418	40.0	37.0	41.0	33.0	41.0
38	37.84249196910669	40.0	37.0	41.0	33.0	41.0
39	37.72312213792632	40.0	37.0	41.0	33.0	41.0
40	37.629006903150845	40.0	36.0	41.0	33.0	41.0
41	37.49875264848609	40.0	36.0	41.0	33.0	41.0
42	37.36494429635705	40.0	36.0	41.0	33.0	41.0
43	36.42874718064384	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	5.0
22	13.0
23	36.0
24	50.0
25	71.0
26	121.0
27	168.0
28	253.0
29	372.0
30	467.0
31	612.0
32	839.0
33	1087.0
34	1614.0
35	2387.0
36	3975.0
37	7565.0
38	15486.0
39	23401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.77390472284874	17.736313307361083	14.300116191647872	25.1896657781423
2	20.499282345704327	19.639805891600027	33.242772196022145	26.6181395666735
3	20.770965757637892	21.510833162463264	31.16840954138473	26.54979153851411
4	15.88066434283371	16.51459230401203	31.27434898503178	36.330394368122484
5	15.737133483698996	34.80794204087212	31.89631604128221	17.558608434146674
6	32.84464493199371	33.037728111543984	15.690998564691409	18.426628391770898
7	28.381518693185704	30.5020162668307	19.311735356434966	21.80472968354863
8	29.869797006356368	31.231631467432162	18.505228624154192	20.393342902057277
9	27.29307634474745	12.666598318638508	18.575285353017566	41.465039983596476
10	20.148998701387466	24.328480623334016	28.600232383295744	26.922288291982778
11	37.37270179755314	20.96917503930012	18.254049620668443	23.404073542478297
12	21.813273187068553	25.950037591415487	26.308864739252275	25.927824482263688
13	34.126170459982234	17.777322124256713	22.790649989747795	25.30585742601326
14	24.255006493062677	20.342081880937734	23.586904517804662	31.816007108194928
15	30.14831522110587	23.85517052833026	20.422390814025015	25.574123436538855
16	26.18071218645342	23.88934454240995	23.103342218576994	26.826601052559635
17	24.03800150365662	26.442143394163075	23.32718201079899	26.192673091381312
18	24.36948944022965	21.844029799740277	25.709110792153645	28.077369967876425
19	26.081607545622308	24.957282482400384	25.21871369011004	23.74239628186727
20	26.65402228145718	21.8303601941084	25.88681566536805	25.628801859066364
21	28.55580616499214	21.565511584990773	23.850044426218304	26.028637823798782
22	28.379809992481718	22.424988039095073	23.67746565511585	25.51773631330736
23	26.443852094867065	22.84361971157132	23.996992686760986	26.71553550680063
24	25.779167521017023	23.578361014284738	24.509602897956395	26.132868566741852
25	26.10040325336614	23.081129109425195	24.109766933223977	26.70870070398469
26	26.519034925842387	22.283165880664342	24.560863919075935	26.636935274417333
27	26.64547877793726	22.870958922835076	24.48226368669264	26.001298612535027
28	25.86801995762422	22.932472148178526	24.83596473241747	26.363543161779784
29	25.758663112569202	23.920101155081678	25.536532021051194	24.78470371129793
30	25.300731323901303	22.761602077780054	26.197799193493264	25.73986740482537
31	25.116191647870963	23.682591757227804	24.777868908481988	26.423347686419245
32	24.940195475360536	23.306677602351172	26.054268334358554	25.69885858792974
33	24.71977308454651	22.826532704531473	26.167042580821544	26.286651630100472
34	25.00512610211195	23.158020641104503	26.175586084341468	25.661267172442077
35	24.721481785250496	22.368600915863578	27.22814571799604	25.68177158088989
36	25.19991798236621	22.27803977855239	25.850932950584376	26.671109288497025
37	23.238329574191784	22.476249060214613	26.893240380015037	27.39218098557857
38	23.470712869933703	21.32458478572893	28.15938760166769	27.045314742669675
39	23.204155560112092	20.97771854282004	29.38110860501675	26.437017292051124
40	22.968354862962205	20.05331146196432	30.543025083726334	26.43530859134714
41	21.591142095550545	20.287403458410225	30.924065340714918	27.19738910532431
42	21.288702070945252	20.946961930148316	31.62292392864466	26.141412070261772
43	20.535165060488005	20.738500444262183	31.250427175176	27.475907320073816
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	3.0
2	5.0
3	18.5
4	32.0
5	32.0
6	45.5
7	59.0
8	62.5
9	66.0
10	87.0
11	108.0
12	108.0
13	199.5
14	291.0
15	491.0
16	691.0
17	590.0
18	489.0
19	489.0
20	529.5
21	570.0
22	419.5
23	269.0
24	264.0
25	259.0
26	259.0
27	317.5
28	376.0
29	421.5
30	467.0
31	492.5
32	518.0
33	518.0
34	664.5
35	811.0
36	962.5
37	1114.0
38	1389.5
39	1665.0
40	1665.0
41	2027.0
42	2389.0
43	2839.5
44	3290.0
45	3774.0
46	4258.0
47	4258.0
48	4827.0
49	5396.0
50	5523.0
51	5650.0
52	5407.0
53	5164.0
54	5164.0
55	5114.5
56	5065.0
57	4855.5
58	4646.0
59	4119.0
60	3592.0
61	3592.0
62	3256.5
63	2921.0
64	2654.5
65	2388.0
66	2210.0
67	2032.0
68	2032.0
69	1737.5
70	1443.0
71	1233.5
72	1024.0
73	856.5
74	689.0
75	689.0
76	543.5
77	398.0
78	309.0
79	220.0
80	169.5
81	119.0
82	119.0
83	76.5
84	34.0
85	24.0
86	14.0
87	7.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	58524.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	77.28965894333949
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.7980014591117	68.63167247624907
2	7.5011606570424245	11.595242977240106
3	1.7664094798045673	4.095755587451301
4	0.6101739880175977	1.886405577199098
5	0.3161408706033206	1.2217210033490533
6	0.24097450976057305	1.117490260405987
7	0.14591117104768644	0.7894197252409267
8	0.08400946211836491	0.5194450140113458
9	0.08400946211836491	0.584375640762764
>10	0.42004731059182454	5.89501742874718
>50	0.01768620255123472	1.013259517462921
>100	0.015475427232330377	2.650194791880254
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	376	0.6424714646982435	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.5160276126033764	No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT	257	0.4391360809240653	TruSeq Adapter, Index 1 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	222	0.3793315562846012	No Hit
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT	169	0.2887704189734126	No Hit
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC	118	0.20162668307019344	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	107	0.18283097532636183	No Hit
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC	95	0.16232656687854555	No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG	92	0.1572004647665915	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	89	0.15207436265463742	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	72	0.12302645068689769	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	70	0.1196090492789283	No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC	63	0.10764814435103548	RNA PCR Primer, Index 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.00170870070398469	0.0
23	0.0	0.0	0.0	0.00512610211195407	0.0
24	0.0	0.0	0.0	0.01196090492789283	0.0
25	0.0	0.0	0.0	0.02221310915180097	0.0
26	0.0	0.0	0.0	0.039300116191647874	0.0
27	0.0	0.0	0.0	0.06663932745540291	0.0
28	0.0	0.0	0.0	0.1742874718064384	0.0
29	0.0	0.0	0.0	0.3810402569885859	0.0
30	0.0	0.0	0.0	0.6851889822978607	0.0
31	0.0	0.0	0.0	1.3567083589638438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTA	20	0.0018276945	37.0	18
CGACATT	20	0.0018276945	37.0	28
GACATTG	20	0.0018276945	37.0	29
TAAGTTC	30	3.5582457E-4	30.833334	30
ATGGCGT	25	0.005454332	29.6	17
GCACGAA	25	0.005454332	29.6	11
ACGAATG	25	0.005454332	29.6	13
GGTAAGT	35	8.7687536E-4	26.42857	28
TCGGGTA	35	8.7687536E-4	26.42857	25
AAGTTCC	35	8.7687536E-4	26.42857	31
AGTTCCG	35	8.7687536E-4	26.42857	32
GGTATCA	205	0.0	25.268293	1
CGGGTAA	40	0.0019094807	23.125	26
CGAAATT	40	0.0019094807	23.125	13
GTCCTAA	40	0.0019094807	23.125	1
CTAAGGT	40	0.0019094807	23.125	4
TCCTAAG	40	0.0019094807	23.125	2
ATTCCTT	40	0.0019094807	23.125	17
GTAAGTT	40	0.0019094807	23.125	29
TACACAT	40	0.0019094807	23.125	5
>>END_MODULE