FastQCFastQC Report
Fri 10 Feb 2017
ERR1632343.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632343.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50369
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT6191.228930492961941No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT6151.2209891004387619RNA PCR Primer, Index 29 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4290.8517143481109413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3270.6492088387698782No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC3110.6174432686771625RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC3040.6035458317615994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT2750.5459707359685522No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG2560.5082491214834521No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC1340.2660366495264945RNA PCR Primer, Index 29 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.23228573130298397No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG1040.2064762056026524No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA840.16676924298675772No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC810.1608131985943735No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG780.15485715420198934No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA690.1369890210248367No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC640.12706228037086303No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG560.11117949532450516No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT540.10720879906291568No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC540.10720879906291568No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC510.10125275467053148No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTC250.005447189329.59999837
TGGGCAG1100.025.2272726
GGGCAGT1150.024.1304367
TACTGTC400.00190579623.12500236
CCGTCTT1053.6379788E-1122.9047637
GCCGTCT1053.6379788E-1122.9047636
TTATACA854.937465E-821.7647062
GCAGTCG1209.094947E-1221.5833349
GGCAGTC1209.094947E-1221.5833348
AGTCGGT1251.6370905E-1120.72000111
CTCTGCA450.003775984720.5555559
ACAGCAC450.003775984720.5555553
ACACAGC450.003775984720.5555551
TCTGCAA450.003775984720.55555510
ATTAAAT450.003775984720.55555535
CACAGCA450.003775984720.5555552
GGACGTA450.003775984720.55555527
CACTCTG555.049853E-420.1818187
CAAACAC555.049853E-420.18181814
CTCGCCT1101.6407284E-920.18181823
Produced by FastQC (version 0.11.5)