##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632343.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50369
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.70390517977327	34.0	31.0	34.0	31.0	34.0
2	32.862931565049934	34.0	31.0	34.0	31.0	34.0
3	32.890746292362365	34.0	31.0	34.0	31.0	34.0
4	36.331255335623105	37.0	37.0	37.0	35.0	37.0
5	36.212154301256724	37.0	35.0	37.0	35.0	37.0
6	36.26407115487701	37.0	37.0	37.0	35.0	37.0
7	36.21938096845282	37.0	36.0	37.0	35.0	37.0
8	36.2488236812325	37.0	36.0	37.0	35.0	37.0
9	38.03527963628422	39.0	38.0	39.0	35.0	39.0
10	37.99805435883182	39.0	38.0	39.0	35.0	39.0
11	38.04353868450833	39.0	38.0	39.0	35.0	39.0
12	37.98586432130874	39.0	38.0	39.0	35.0	39.0
13	38.053703666938	39.0	38.0	39.0	35.0	39.0
14	39.32404852190832	40.0	39.0	41.0	37.0	41.0
15	39.35660823125335	40.0	39.0	41.0	37.0	41.0
16	39.30286485735274	40.0	39.0	41.0	36.0	41.0
17	39.28843137644186	40.0	39.0	41.0	36.0	41.0
18	39.407413289920385	40.0	39.0	41.0	37.0	41.0
19	39.425718199686315	40.0	39.0	41.0	37.0	41.0
20	39.43002640513014	40.0	39.0	41.0	37.0	41.0
21	39.44154142428875	40.0	39.0	41.0	37.0	41.0
22	39.34102324842661	40.0	39.0	41.0	36.0	41.0
23	39.33431277174453	40.0	39.0	41.0	36.0	41.0
24	39.252476721793165	40.0	39.0	41.0	36.0	41.0
25	39.17127598324366	40.0	39.0	41.0	36.0	41.0
26	39.07816315590939	40.0	39.0	41.0	36.0	41.0
27	38.95086263376283	40.0	38.0	41.0	35.0	41.0
28	38.89513391173142	40.0	38.0	41.0	35.0	41.0
29	38.81446921717723	40.0	38.0	41.0	35.0	41.0
30	38.70467946554428	40.0	38.0	41.0	35.0	41.0
31	38.54068970994064	40.0	38.0	41.0	35.0	41.0
32	38.415453949850104	40.0	38.0	41.0	35.0	41.0
33	38.29855665190891	40.0	38.0	41.0	34.0	41.0
34	38.17838352955191	40.0	37.0	41.0	34.0	41.0
35	38.16180587265977	40.0	37.0	41.0	34.0	41.0
36	38.06964601242828	40.0	37.0	41.0	34.0	41.0
37	37.95517083920665	40.0	37.0	41.0	34.0	41.0
38	37.86277273719947	40.0	37.0	41.0	33.0	41.0
39	37.76275089837003	40.0	37.0	41.0	33.0	41.0
40	37.61244019138756	40.0	36.0	41.0	33.0	41.0
41	37.46729138954516	40.0	36.0	41.0	33.0	41.0
42	37.32514046338025	40.0	36.0	41.0	33.0	41.0
43	36.3986976116262	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	0.0
17	0.0
18	0.0
19	5.0
20	3.0
21	7.0
22	10.0
23	26.0
24	29.0
25	53.0
26	93.0
27	148.0
28	198.0
29	294.0
30	348.0
31	558.0
32	725.0
33	969.0
34	1389.0
35	2026.0
36	3372.0
37	6744.0
38	13359.0
39	20011.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.57120451071095	17.25068990847545	15.291151303381048	25.88695427743255
2	19.936865929440728	19.980543588318213	33.29031745716611	26.792273025074948
3	20.038118684111257	21.74154738033314	33.6337826837936	24.586551251761996
4	14.423554170223749	17.90386944350692	30.975401536659454	36.69717484960988
5	14.6300303758264	34.95006849451051	32.422720324008814	17.997180805654274
6	31.64644920486807	33.28237606464294	16.166689829061525	18.904484901427466
7	26.69300561853521	32.114991363735626	18.040858464531755	23.151144553197405
8	31.275189104409456	30.39965057872898	17.985268716869506	20.339891599992058
9	26.93918878675376	12.523576009053187	17.37576684071552	43.16146836347753
10	20.447100399054975	24.89030951577359	26.4329250134011	28.22966507177033
11	35.160515396374755	21.78721038734142	17.760924378089697	25.291349838194126
12	21.818975957434137	28.412317099803452	24.41978200877524	25.348924933987178
13	34.586749786575076	17.25068990847545	23.12533502749707	25.0372252774524
14	24.175584188687484	21.499334908376184	22.509877106950704	31.815203795985624
15	30.421489408167723	24.572653814846433	19.559649784589727	25.446206992396114
16	27.21316682880343	23.512477913002044	23.008199487780182	26.26615577041434
17	21.92618475649705	29.8655919315452	22.45825805555004	25.74996525640771
18	24.507137326530206	21.292858702773533	25.20002382417757	28.999980146518695
19	25.090829676983862	25.6050348428597	26.363437828823283	22.94069765133316
20	27.556632055430917	20.866008854652662	25.777760130238836	25.799598959677578
21	29.62337945958824	20.48680736167087	24.02668307887788	25.863130099863014
22	28.708133971291865	24.753320494748753	22.396712263495406	24.141833270463977
23	26.935218090492167	22.186265361631165	23.574023705056682	27.30449284281999
24	25.360837022771943	24.15374535924874	25.11862455081498	25.366793067164327
25	25.27943774940936	24.171613492425898	24.397943179336494	26.151005578828247
26	26.76447815124382	22.92878556254839	23.92543032420735	26.381305962000432
27	25.34296888959479	24.598463340546765	24.443606186344777	25.61496158351367
28	24.040580515793444	22.688558438722232	26.1311520975203	27.139708947964024
29	24.576624511108022	23.041950406003693	27.495086263376283	24.886338819512
30	25.472016518096446	23.000258095257003	28.18598741289285	23.3417379737537
31	23.760646429351386	24.04256586392424	24.76126188727193	27.43552581945244
32	24.036609819531854	24.78508606484147	27.431555123190854	23.746748992435823
33	23.85792848776033	23.417181202723896	28.213782286723976	24.5111080227918
34	23.939327761122914	24.477357104568288	25.567313228374594	26.016001905934203
35	22.509877106950704	23.11937898310469	30.097877662848184	24.272866247096427
36	23.691159244773573	24.20337906251861	25.926661240048443	26.178800452659374
37	22.02942285929838	23.92940102046894	27.326331672258732	26.714844447973952
38	22.50392106255832	21.294844050904327	29.26403144791439	26.937203438622966
39	23.23452917469078	20.955349520538427	29.36726955071572	26.442851754055074
40	21.737576684071552	20.00238241775695	32.21624411840616	26.043796779765334
41	20.727034485497033	21.074470408386112	30.488991244614745	27.709503861502117
42	20.568206635033455	20.566221286902657	32.901189223530345	25.96438285453354
43	18.979928130397667	20.840199328952334	32.780082987551864	27.399789553098135
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	16.5
4	28.0
5	28.0
6	49.5
7	71.0
8	71.0
9	71.0
10	96.5
11	122.0
12	122.0
13	239.5
14	357.0
15	583.5
16	810.0
17	681.0
18	552.0
19	552.0
20	593.5
21	635.0
22	442.5
23	250.0
24	239.5
25	229.0
26	229.0
27	267.5
28	306.0
29	339.5
30	373.0
31	419.0
32	465.0
33	465.0
34	525.0
35	585.0
36	685.0
37	785.0
38	1029.5
39	1274.0
40	1274.0
41	1530.5
42	1787.0
43	2211.0
44	2635.0
45	3180.5
46	3726.0
47	3726.0
48	4577.5
49	5429.0
50	5559.0
51	5689.0
52	5071.5
53	4454.0
54	4454.0
55	4434.5
56	4415.0
57	4260.0
58	4105.0
59	3405.0
60	2705.0
61	2705.0
62	2472.0
63	2239.0
64	2026.0
65	1813.0
66	1649.0
67	1485.0
68	1485.0
69	1274.5
70	1064.0
71	914.0
72	764.0
73	634.5
74	505.0
75	505.0
76	415.0
77	325.0
78	249.5
79	174.0
80	132.0
81	90.0
82	90.0
83	61.5
84	33.0
85	20.5
86	8.0
87	5.5
88	3.0
89	3.0
90	2.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	50369.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.54078897734718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02066976903532	61.21026822053247
2	7.0716875553144725	9.835414639957117
3	1.9356496417049704	4.03819809803649
4	1.0077939874953608	2.8033115606821655
5	0.4653552973420504	1.618058726597709
6	0.2912039283980929	1.2150330560463778
7	0.19699089273988638	0.9589231471738568
8	0.1541667856225198	0.8576703925033254
9	0.1227624404031176	0.7683297266175624
>10	0.6766208924543924	8.590601361948817
>50	0.025694464270419957	1.1733407452996882
>100	0.025694464270419957	4.480930731203717
>500	0.005709880948982214	2.4499195934007028
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	619	1.228930492961941	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	615	1.2209891004387619	RNA PCR Primer, Index 29 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	429	0.8517143481109413	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	327	0.6492088387698782	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	311	0.6174432686771625	RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	304	0.6035458317615994	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	275	0.5459707359685522	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	256	0.5082491214834521	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	134	0.2660366495264945	RNA PCR Primer, Index 29 (95% over 24bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.23228573130298397	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	104	0.2064762056026524	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	84	0.16676924298675772	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	81	0.1608131985943735	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	78	0.15485715420198934	No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA	69	0.1369890210248367	No Hit
GGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGC	64	0.12706228037086303	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	56	0.11117949532450516	No Hit
TCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCTCT	54	0.10720879906291568	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	54	0.10720879906291568	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	51	0.10125275467053148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.001985348130794735	0.0
18	0.0	0.0	0.0	0.001985348130794735	0.0
19	0.0	0.0	0.0	0.001985348130794735	0.0
20	0.0	0.0	0.0	0.005956044392384205	0.0
21	0.0	0.0	0.0	0.01588278504635788	0.0
22	0.0	0.0	0.0	0.025809525700331553	0.0
23	0.0	0.0	0.0	0.03772161448509996	0.0
24	0.0	0.0	0.0	0.06551648831622625	0.0
25	0.0	0.0	0.0	0.07544322897019992	0.0
26	0.0	0.0	0.0	0.08735531775496834	0.0
27	0.0	0.0	0.0	0.12309158410927357	0.0
28	0.0	0.001985348130794735	0.0	0.20052016121026822	0.0
29	0.0	0.001985348130794735	0.0	0.4069963668129206	0.0
30	0.0	0.001985348130794735	0.0	0.7623736822251782	0.0
31	0.0	0.001985348130794735	0.0	1.5168059719271774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTC	25	0.0054471893	29.599998	37
TGGGCAG	110	0.0	25.227272	6
GGGCAGT	115	0.0	24.130436	7
TACTGTC	40	0.001905796	23.125002	36
CCGTCTT	105	3.6379788E-11	22.90476	37
GCCGTCT	105	3.6379788E-11	22.90476	36
TTATACA	85	4.937465E-8	21.764706	2
GCAGTCG	120	9.094947E-12	21.583334	9
GGCAGTC	120	9.094947E-12	21.583334	8
AGTCGGT	125	1.6370905E-11	20.720001	11
CTCTGCA	45	0.0037759847	20.555555	9
ACAGCAC	45	0.0037759847	20.555555	3
ACACAGC	45	0.0037759847	20.555555	1
TCTGCAA	45	0.0037759847	20.555555	10
ATTAAAT	45	0.0037759847	20.555555	35
CACAGCA	45	0.0037759847	20.555555	2
GGACGTA	45	0.0037759847	20.555555	27
CACTCTG	55	5.049853E-4	20.181818	7
CAAACAC	55	5.049853E-4	20.181818	14
CTCGCCT	110	1.6407284E-9	20.181818	23
>>END_MODULE