FastQCFastQC Report
Fri 10 Feb 2017
ERR1632342.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632342.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT2015.577136514983351TruSeq Adapter, Index 8 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC832.3029966703662597No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT772.1365149833518315No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC621.7203107658157604RNA PCR Primer, Index 8 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC581.609322974472808No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG441.2208657047724751No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG320.8879023307436182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140.388457269700333No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC110.3052164261931188No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT90.24972253052164264No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC90.24972253052164264No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG90.24972253052164264No Hit
ATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCT90.24972253052164264TruSeq Adapter, Index 8 (95% over 21bp)
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG90.24972253052164264No Hit
ACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGT80.22197558268590456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70.1942286348501665No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGC70.1942286348501665No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC60.1664816870144284No Hit
CCTTAGGACCGTTATTGTTACGGCCGCCGTTTACTGGGACTTC60.1664816870144284No Hit
TCCTGAGACTCAGCGAAGTTGAAATGTTTGTGATGATGCAATC60.1664816870144284No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA60.1664816870144284No Hit
ACTCAGGAGAAGGAACTCGGCAAATTGACACCGTAACTTCGGA50.13873473917869034No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50.13873473917869034No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAG50.13873473917869034No Hit
ATTCTAAGGCGCTTGAGAGAACTCAGGAGAAGGAACTCGGCAA40.11098779134295228No Hit
CGTATAGGGTGTGACGCCTGCCCGGTGCTGGAAGATTAAATGA40.11098779134295228No Hit
GGAGAAGGAACTCGGCAAATTGACACCGTAACTTCGGAAGAAG40.11098779134295228No Hit
GGGTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTA40.11098779134295228No Hit
GGCAACGACCGTACCGCAAACCGACACTGGTGCGCGAGATGAG40.11098779134295228No Hit
CATTTCAACTTCGCTGAGTCTCAGGAGGAGACAGTGTGGCCAT40.11098779134295228No Hit
TTCCAGCACCGGGCAGGCGTCACACCCTATACGTCCACTTTCG40.11098779134295228No Hit
ACCGTACCGCAAACCGACACTGGTGCGCGAGATGAGTATTCTA40.11098779134295228No Hit
TGAGAGAACTCAGGAGAAGGAACTCGGCAAATTGACACCGTAA40.11098779134295228No Hit
GAGATTGCATCATCACAAACATTTCAACTTCGCTGAGTCTCAG40.11098779134295228No Hit
ACACAGCACTCTGCAAACACGAAAGTGGACGTATAGGGTGTGA40.11098779134295228No Hit
TGAATGGGTTGCAAAAAATCGGTGGCTGCGACTGTTTATTAAA40.11098779134295228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT250.004808238229.59999837
GCCGTCT250.004808238229.59999836
Produced by FastQC (version 0.11.5)