FastQCFastQC Report
Fri 10 Feb 2017
ERR1632341.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632341.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9211
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT4244.603191835848442TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT2813.050700249701444No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC1551.6827706003691238No Hit
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC1441.5633481706655086No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG1251.3570730648138096No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC780.8468135924438172RNA PCR Primer, Index 6 (95% over 22bp)
CACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCG440.477689718814461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT370.40169362718488766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300.3256975355553143No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240.26055802844425147No Hit
ATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCT200.21713169037020952RNA PCR Primer, Index 40 (95% over 21bp)
ACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGT180.19541852133318857No Hit
TTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTA130.1411355987406362No Hit
CACTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTT120.13027901422212573No Hit
TCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTCT110.11942242970361525RNA PCR Primer, Index 40 (95% over 24bp)
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC100.10856584518510476No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC100.10856584518510476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC250.005221129429.5999983
TTATACA250.005221129429.5999982
CTTATAC250.005221129429.5999981
CCGTCTT500.00653062718.49999837
GCCGTCT500.00653062718.49999836
Produced by FastQC (version 0.11.5)