ERR1632339.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1632339.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3181
Sequences flagged as poor quality	0
Sequence length	43
%GC	52

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	184	5.784344545740333	TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	92	2.8921722728701664	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT	77	2.42062244577177	No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC	57	1.7918893429739076	No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG	37	1.1631562401760454	No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC	33	1.0374096196164728	RNA PCR Primer, Index 3 (95% over 22bp)
GTGTGGTACTGAGCTGACGACAAGTAGGGCGGGACACGAGAAA	11	0.3458032065388243	No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG	10	0.31436655139893116	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.22005658597925182	No Hit
CAATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT	6	0.1886199308393587	No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC	6	0.1886199308393587	No Hit
GTGATAGCACAAGACTTAACGGAACAGTTTGGAAAGACTGGCC	5	0.15718327569946558	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.15718327569946558	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.12574662055957245	No Hit
GACATCAACCGAGATTCCGAAAGTAGTGGCGAGCGAAATCGGA	4	0.12574662055957245	No Hit
CTAATAGGGCACACCTTCTTCCGAAGTTACGGTGTCAATTTGC	4	0.12574662055957245	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	4	0.12574662055957245	No Hit
CTTTACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA	4	0.12574662055957245	No Hit
CAGTACCACACAGGTCTTTTCACGTACGGGGCTATCACCCACT	4	0.12574662055957245	No Hit
GATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT	4	0.12574662055957245	No Hit
GTGAATAAGAGCACGTGGTGGATGCCTTGGCGATGATAGGCGA	4	0.12574662055957245	No Hit
ATCACTGACAGGCTCTTCCGATTTCGCTCGCCACTACTTTCGG	4	0.12574662055957245	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	4	0.12574662055957245	No Hit
GTGCAAGCTCTTGATTGAAGTCCCAGTAAACGGCGGCCGTAAC	4	0.12574662055957245	No Hit
GTCTTGTGCTATCACTGACAGGCTCTTCCGATTTCGCTCGCCA	4	0.12574662055957245	No Hit
ATCAAGAGCTTGCACCCCATCATTTAATCTTCCAGCACCGGGC	4	0.12574662055957245	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	4	0.12574662055957245	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	25	9.764299E-5	37.0	35
TATGCCG	25	9.764299E-5	37.0	33
CCGTCTT	25	9.764299E-5	37.0	37
ATGCCGT	25	9.764299E-5	37.0	34
GCCGTCT	25	9.764299E-5	37.0	36
GTATGCC	30	2.855152E-4	30.833332	32
TCTCGTA	30	2.855152E-4	30.833332	28
CGTATGC	30	2.855152E-4	30.833332	31
TCGTATG	30	2.855152E-4	30.833332	30
CTCGTAT	30	2.855152E-4	30.833332	29
GAGGAAT	50	1.9644873E-4	22.2	22
AGAGGAA	50	1.9644873E-4	22.2	21
AGCCCAC	45	0.0030540982	20.555557	7
GTAGAGG	45	0.0030540982	20.555557	19
AATCTCG	45	0.0030540982	20.555557	26
ATCTCGT	45	0.0030540982	20.555557	27
GCCCACG	45	0.0030540982	20.555557	8
CCACGAG	45	0.0030540982	20.555557	10
AGGAATC	45	0.0030540982	20.555557	23
ACGTAGA	45	0.0030540982	20.555557	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content