FastQCFastQC Report
Fri 10 Feb 2017
ERR1632338.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632338.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19121
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT4082.1337796140369227RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT3221.6840123424507087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2061.0773495110088385No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC2051.07211965901365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1910.9989017310810104No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC1680.8786151351916741No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG1380.7217195753360179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1370.7164897233408294No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC890.4654568275717797RNA PCR Primer, Index 41 (95% over 23bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG630.3294806756968778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT580.3033314157209351No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400.20919407980754143No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG210.10982689189895926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACAT551.6625563E-830.2727285
TATACAC502.4919973E-729.63
TTATACA555.7383477E-726.909092
ATACACA555.7383477E-726.909094
CCGTCTT508.495945E-625.90000237
GCCGTCT451.2579106E-424.66666636
GGTATCA751.2030796E-824.6666661
CTTATAC652.46126E-622.769231
ACACATC801.485539E-518.56
GTATCAA1054.2184183E-717.6190472
TGCCGTC700.002469560415.85714335
ATGCCGT750.00391134814.834
CACATCT1102.2643062E-413.4545457
AACGCAG1551.6266531E-613.1290325
CCTGTCT1153.299209E-412.86956537
ATCAACG1602.3242792E-612.718752
CAACGCA1602.3242792E-612.718754
AGTACAT1256.664719E-411.8414
GAGTACA1256.664719E-411.8413
TCAACGC1756.3374246E-611.62857153
Produced by FastQC (version 0.11.5)