##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632338.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19121
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.657235500235345	34.0	31.0	34.0	31.0	34.0
2	32.80157941530255	34.0	31.0	34.0	31.0	34.0
3	32.85340724857487	34.0	31.0	34.0	31.0	34.0
4	36.28167982846085	37.0	37.0	37.0	35.0	37.0
5	36.17676899743737	37.0	35.0	37.0	35.0	37.0
6	36.209298676847446	37.0	36.0	37.0	35.0	37.0
7	36.18733329846766	37.0	36.0	37.0	35.0	37.0
8	36.20009413733592	37.0	36.0	37.0	35.0	37.0
9	37.96898697766853	39.0	38.0	39.0	35.0	39.0
10	37.943883688091624	39.0	38.0	39.0	35.0	39.0
11	38.00617122535432	39.0	38.0	39.0	35.0	39.0
12	37.947439987448355	39.0	38.0	39.0	35.0	39.0
13	38.02552167773652	39.0	38.0	39.0	35.0	39.0
14	39.26891898959259	40.0	39.0	41.0	36.0	41.0
15	39.30280843052142	40.0	39.0	41.0	36.0	41.0
16	39.249254746090685	40.0	39.0	41.0	36.0	41.0
17	39.23278071230584	40.0	39.0	41.0	36.0	41.0
18	39.34302599236442	40.0	39.0	41.0	37.0	41.0
19	39.37822289629204	40.0	39.0	41.0	37.0	41.0
20	39.39438313895717	40.0	39.0	41.0	37.0	41.0
21	39.37309764133675	40.0	39.0	41.0	36.0	41.0
22	39.24805188013179	40.0	39.0	41.0	36.0	41.0
23	39.18905914962607	40.0	39.0	41.0	36.0	41.0
24	39.121123372208565	40.0	39.0	41.0	36.0	41.0
25	39.03509230688771	40.0	38.0	41.0	35.0	41.0
26	38.960514617436324	40.0	38.0	41.0	35.0	41.0
27	38.80168401234245	40.0	38.0	41.0	35.0	41.0
28	38.7517389257884	40.0	38.0	41.0	35.0	41.0
29	38.62224779038753	40.0	38.0	41.0	35.0	41.0
30	38.507557136133045	40.0	38.0	41.0	35.0	41.0
31	38.35531614455311	40.0	38.0	41.0	35.0	41.0
32	38.15360075309869	40.0	37.0	41.0	34.0	41.0
33	37.99147534124784	40.0	37.0	41.0	34.0	41.0
34	37.91783902515559	40.0	37.0	41.0	34.0	41.0
35	37.8351027665917	40.0	37.0	41.0	33.0	41.0
36	37.74839182051148	40.0	36.0	41.0	33.0	41.0
37	37.6463051095654	40.0	36.0	41.0	33.0	41.0
38	37.524083468437844	40.0	36.0	41.0	33.0	41.0
39	37.36342241514565	40.0	36.0	41.0	33.0	41.0
40	37.10982689189896	39.0	35.0	41.0	32.0	41.0
41	36.951623869044504	39.0	35.0	41.0	32.0	41.0
42	36.83829297630877	39.0	35.0	41.0	32.0	41.0
43	35.89634433345536	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	2.0
21	2.0
22	1.0
23	3.0
24	21.0
25	21.0
26	41.0
27	77.0
28	97.0
29	153.0
30	176.0
31	238.0
32	349.0
33	426.0
34	627.0
35	870.0
36	1297.0
37	2647.0
38	4764.0
39	7309.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.2384812509806	18.712410438784584	16.050415773233617	24.9986925370012
2	18.63919251085194	20.7572825689033	33.8476021128602	26.755922807384554
3	19.350452382197584	23.15778463469484	33.565190105120024	23.926572877987553
4	14.591287066576017	17.98023115945819	29.878144448512106	37.55033732545369
5	14.240886982898385	34.84650384394122	32.47215103812562	18.440458135034778
6	31.22221641127556	33.246169133413524	15.956278437320224	19.57533601799069
7	25.903456932168822	31.92824643062601	18.785628366717223	23.382668270487944
8	30.6103237278385	30.254693792165682	19.319073270226454	19.815909209769362
9	25.291564248731763	12.875895612154176	18.278332723183937	43.55420741593013
10	19.972804769625018	25.997594268082214	26.567648135557764	27.461952826735004
11	33.607028921081536	21.405784216306678	18.65488206683751	26.33230479577428
12	20.171539145442182	29.50159510485853	25.00392238899639	25.322943360702894
13	33.29846765336541	17.640290779770933	23.926572877987553	25.134668688876104
14	24.05208932587208	22.085664975681187	23.712148946184822	30.150096752261913
15	28.241200774018093	25.76225092829873	20.307515297317085	25.68903300036609
16	26.954657183201714	23.889963914021234	23.44019664243502	25.715182260342033
17	20.24998692537001	31.687673238847342	22.77077558705089	25.291564248731763
18	23.79582657810784	21.40055436431149	26.018513676062966	28.7851053815177
19	23.508184718372473	25.19219706082318	26.05512264002929	25.244495580775062
20	24.245593849694053	22.04382615971968	27.095863187071807	26.61471680351446
21	27.86988128235971	21.452852884263375	26.280006275822394	24.39725955755452
22	25.505988180534487	27.676376758537735	23.8585848020501	22.959050258877674
23	24.167146069766225	24.726740233251398	25.495528476544116	25.61058522043826
24	26.02374352805816	24.742429789236965	25.239265728779873	23.994560953925003
25	22.420375503373254	27.801893206422257	24.857486533131112	24.920244757073377
26	25.495528476544116	22.373306835416557	26.04466293603891	26.08650175200042
27	22.43606505935882	28.021546990220177	25.176507504837613	24.36588044558339
28	22.038596307724493	23.989331101929814	28.581141153705353	25.39093143664034
29	23.210083154646725	24.496626745463104	26.243397311856075	26.0498927880341
30	21.384864808325922	25.521677736520054	28.968150201349303	24.125307253804717
31	22.598190471209666	23.87427435803567	27.472412530725382	26.05512264002929
32	23.241462266617855	27.341666230845668	27.430573714763874	21.986297787772607
33	21.33256628837404	25.4118508446211	29.710789184666076	23.544793682338792
34	24.08346843784321	25.53736729250562	26.714083991423042	23.665080278228125
35	21.03446472464829	24.9986925370012	31.713822498823284	22.25302023952722
36	22.932900998901733	23.638931018252183	26.164949531928244	27.263218450917837
37	20.74682286491292	23.9056534700068	29.015218869305997	26.33230479577428
38	21.275037916426964	22.211181423565712	30.41158935202134	26.102191307985983
39	22.943360702892107	22.57727106322891	29.402227916949954	25.077140316929032
40	20.18199884943256	21.32210658438366	34.01495737670624	24.48093718947754
41	20.846190052821505	21.82940222791695	29.940902672454367	27.383505046807176
42	20.380733225249724	21.07107368861461	33.471052769206636	25.077140316929032
43	18.921604518592126	21.620208148109405	32.88530934574552	26.57287798755295
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	8.5
4	17.0
5	17.0
6	21.5
7	26.0
8	33.5
9	41.0
10	57.5
11	74.0
12	74.0
13	138.5
14	203.0
15	304.0
16	405.0
17	365.5
18	326.0
19	326.0
20	332.5
21	339.0
22	251.0
23	163.0
24	152.5
25	142.0
26	142.0
27	164.5
28	187.0
29	199.0
30	211.0
31	231.5
32	252.0
33	252.0
34	278.0
35	304.0
36	344.0
37	384.0
38	482.0
39	580.0
40	580.0
41	656.5
42	733.0
43	885.0
44	1037.0
45	1164.5
46	1292.0
47	1292.0
48	1496.0
49	1700.0
50	1866.5
51	2033.0
52	1758.5
53	1484.0
54	1484.0
55	1707.5
56	1931.0
57	1737.0
58	1543.0
59	1265.5
60	988.0
61	988.0
62	861.0
63	734.0
64	651.5
65	569.0
66	505.0
67	441.0
68	441.0
69	403.0
70	365.0
71	301.5
72	238.0
73	201.0
74	164.0
75	164.0
76	140.5
77	117.0
78	86.5
79	56.0
80	39.0
81	22.0
82	22.0
83	15.5
84	9.0
85	7.5
86	6.0
87	4.5
88	3.0
89	3.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	19121.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.9636002301135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.6368330464716	68.85100151665708
2	6.024096385542169	9.152240991579937
3	1.6041308089500863	3.655666544636787
4	0.7228915662650602	2.1965378379791853
5	0.2753872633390706	1.0459703990377074
6	0.17900172117039587	0.8158569112494116
7	0.1376936316695353	0.732179279326395
8	0.08261617900172118	0.5020657915380995
9	0.06196213425129088	0.4236180116102714
>10	0.19965576592082618	2.2436065059358823
>50	0.020654044750430294	1.0982689189895927
>100	0.055077452667814115	9.282987291459651
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT	408	2.1337796140369227	RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT	322	1.6840123424507087	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	206	1.0773495110088385	No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC	205	1.07211965901365	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	191	0.9989017310810104	No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC	168	0.8786151351916741	No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG	138	0.7217195753360179	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	137	0.7164897233408294	No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC	89	0.4654568275717797	RNA PCR Primer, Index 41 (95% over 23bp)
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG	63	0.3294806756968778	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	58	0.3033314157209351	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	0.20919407980754143	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	21	0.10982689189895926	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.005229851995188536	0.0
19	0.0	0.0	0.0	0.005229851995188536	0.0
20	0.0	0.0	0.0	0.005229851995188536	0.0
21	0.0	0.0	0.0	0.005229851995188536	0.0
22	0.0	0.0	0.0	0.010459703990377073	0.0
23	0.0	0.0	0.0	0.020919407980754146	0.0
24	0.0	0.0	0.0	0.03137911197113122	0.0
25	0.0	0.0	0.0	0.036608963966319755	0.0
26	0.0	0.0	0.0	0.04706866795669683	0.0
27	0.0	0.0	0.0	0.09413733591339365	0.0
28	0.0	0.0	0.0	0.19350452382197583	0.0
29	0.0	0.0	0.0	0.3504000836776319	0.0
30	0.0	0.0	0.0	0.6380419434130015	0.0
31	0.0	0.0	0.0	1.229015218869306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACACAT	55	1.6625563E-8	30.272728	5
TATACAC	50	2.4919973E-7	29.6	3
TTATACA	55	5.7383477E-7	26.90909	2
ATACACA	55	5.7383477E-7	26.90909	4
CCGTCTT	50	8.495945E-6	25.900002	37
GCCGTCT	45	1.2579106E-4	24.666666	36
GGTATCA	75	1.2030796E-8	24.666666	1
CTTATAC	65	2.46126E-6	22.76923	1
ACACATC	80	1.485539E-5	18.5	6
GTATCAA	105	4.2184183E-7	17.619047	2
TGCCGTC	70	0.0024695604	15.857143	35
ATGCCGT	75	0.003911348	14.8	34
CACATCT	110	2.2643062E-4	13.454545	7
AACGCAG	155	1.6266531E-6	13.129032	5
CCTGTCT	115	3.299209E-4	12.869565	37
ATCAACG	160	2.3242792E-6	12.71875	2
CAACGCA	160	2.3242792E-6	12.71875	4
AGTACAT	125	6.664719E-4	11.84	14
GAGTACA	125	6.664719E-4	11.84	13
TCAACGC	175	6.3374246E-6	11.6285715	3
>>END_MODULE