FastQCFastQC Report
Fri 10 Feb 2017
ERR1632337.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632337.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139919
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT14521.0377432657466106No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13900.9934319141789179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT8690.6210736211665321No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.3680700976993832No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT4710.33662333207069806RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT3140.2244155547137987No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC2410.17224251173893465No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC2210.1579485273622596No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2090.14937213673625455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.11649597266990189No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG1550.11077837891923184No Hit
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGA1460.10434608594972807No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTT303.5834822E-430.8333347
CGTTATT250.005480061829.610
ATACTAT250.005480061829.61
AACATGG250.005480061829.620
TTAGGAC405.903482E-527.753
GGACCGT358.830354E-426.428576
CAGGGTG400.001922767123.1254
ACCGTTA400.001922767123.1258
TAGGACC400.001922767123.1254
GGTATCA6500.021.9153841
CTCTGCA603.6962632E-521.5833349
AATGGGA450.003809286520.55555520
GACGCGC450.003809286520.5555556
CCGTTTA450.003809286520.55555527
CCGTTAT450.003809286520.5555559
GCCGTCT759.170757E-619.73333436
TCTGCAA500.007004793718.510
CCTTATT500.007004793718.527
TTACTGG609.1816555E-418.531
ACGGCCG500.007004793718.520
Produced by FastQC (version 0.11.5)