##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632337.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139919
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45482028888142	33.0	31.0	34.0	31.0	34.0
2	32.60247714749248	34.0	31.0	34.0	31.0	34.0
3	32.646152416755406	34.0	31.0	34.0	31.0	34.0
4	36.16963385959019	37.0	35.0	37.0	35.0	37.0
5	36.01503727156426	37.0	35.0	37.0	35.0	37.0
6	36.0704121670395	37.0	35.0	37.0	35.0	37.0
7	36.05026479606058	37.0	35.0	37.0	35.0	37.0
8	36.06237180082762	37.0	35.0	37.0	35.0	37.0
9	37.80290739642221	39.0	38.0	39.0	35.0	39.0
10	37.72850006074943	39.0	37.0	39.0	35.0	39.0
11	37.80604492599289	39.0	37.0	39.0	35.0	39.0
12	37.759861062471856	39.0	37.0	39.0	35.0	39.0
13	37.809568393141745	39.0	37.0	39.0	35.0	39.0
14	39.061542749733775	40.0	38.0	41.0	36.0	41.0
15	39.07342819774298	40.0	38.0	41.0	36.0	41.0
16	39.01774598160364	40.0	38.0	41.0	36.0	41.0
17	39.03304054488668	40.0	38.0	41.0	36.0	41.0
18	39.07755915922784	40.0	38.0	41.0	36.0	41.0
19	39.09157441090917	40.0	39.0	41.0	36.0	41.0
20	39.090523803057486	40.0	39.0	41.0	36.0	41.0
21	39.08717901071334	40.0	39.0	41.0	36.0	41.0
22	39.009269648868276	40.0	38.0	41.0	36.0	41.0
23	38.947798369056386	40.0	38.0	41.0	35.0	41.0
24	38.88376131904888	40.0	38.0	41.0	35.0	41.0
25	38.83609802814485	40.0	38.0	41.0	35.0	41.0
26	38.69358700390941	40.0	38.0	41.0	35.0	41.0
27	38.57867051651313	40.0	38.0	41.0	35.0	41.0
28	38.50124000314467	40.0	38.0	41.0	35.0	41.0
29	38.38148500203689	40.0	38.0	41.0	34.0	41.0
30	38.24359093475511	40.0	38.0	41.0	34.0	41.0
31	38.10601133512961	40.0	37.0	41.0	34.0	41.0
32	37.971176180504436	40.0	37.0	41.0	34.0	41.0
33	37.82987299794881	40.0	37.0	41.0	33.0	41.0
34	37.69667450453477	40.0	37.0	41.0	33.0	41.0
35	37.61618507850971	40.0	36.0	41.0	33.0	41.0
36	37.52215924927994	40.0	36.0	41.0	33.0	41.0
37	37.404591227781786	40.0	36.0	41.0	33.0	41.0
38	37.297129053237946	40.0	36.0	41.0	33.0	41.0
39	37.15288845689292	39.0	36.0	41.0	32.0	41.0
40	36.99447537503841	39.0	35.0	41.0	32.0	41.0
41	36.84191567978616	39.0	35.0	41.0	31.0	41.0
42	36.686161279025725	39.0	35.0	41.0	31.0	41.0
43	35.7465390690328	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	5.0
19	3.0
20	14.0
21	27.0
22	44.0
23	77.0
24	145.0
25	214.0
26	375.0
27	586.0
28	841.0
29	1179.0
30	1618.0
31	2000.0
32	2667.0
33	3415.0
34	4771.0
35	6839.0
36	10936.0
37	20816.0
38	39948.0
39	43397.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.35865750898734	18.661511302968144	13.845867966466313	24.1339632215782
2	19.997284142968432	20.584052201630943	33.58014279690392	25.8385208584967
3	21.378083033755242	22.592357006553794	30.49335687076094	25.536203088930026
4	15.83487589248065	16.72038822461567	31.592564269327255	35.85217161357643
5	15.725526911999085	35.2525389689749	31.75908918731552	17.26284493171049
6	32.6374545272622	32.72536253117875	16.43665263473867	18.200530306820376
7	27.766064651691337	31.172321128653007	19.91723783045905	21.144376389196605
8	29.639291304254606	31.17517992552834	19.170377146777778	20.015151623439277
9	27.36583308914443	12.871732931195906	19.908661439833047	39.853772539826615
10	19.53272965072649	25.79992710067968	29.259071319835044	25.40827192875878
11	35.28398573460359	21.749726627548796	19.163230154589442	23.803057483258172
12	22.022027029924455	27.337245120391085	26.415997827314374	24.224730022370085
13	32.895460945261185	18.62434694358879	24.090366569229342	24.389825541920683
14	24.235450510652594	21.06361537746839	24.902264881824486	29.798669230054532
15	28.807381413532113	24.918702963857662	22.21499581900957	24.058919803600652
16	25.048063522466567	24.281905959876788	24.61281169819681	26.05721881945983
17	23.03332642457422	27.172864300059317	25.008755065430716	24.785054209935748
18	23.537189373852012	23.156969389432458	27.11282956567728	26.193011671038242
19	24.697146206019198	25.414704221728286	27.749626569658158	22.138523002594358
20	25.00589626855538	22.892530678463967	28.05623253453784	24.045340518442814
21	27.458743987592822	22.463711147163718	26.307363546051644	23.77018131919182
22	26.479606057790576	23.735161057468964	25.580514440497716	24.20471844424274
23	25.544064780337195	23.826642557479687	26.158706108534226	24.470586553648896
24	24.918702963857662	24.725734174772544	26.164423702284896	24.191139159084898
25	24.527762491155595	24.27190017081311	26.24804351088844	24.952293827142846
26	25.151694909197463	23.702284893402613	26.573231655457803	24.572788541942124
27	24.274044268469613	24.185421565334227	26.773347436731253	24.767186729464903
28	24.15326010048671	24.317640920818473	27.133555843023466	24.395543135671353
29	24.612096998977982	24.853665334943788	26.951307542220853	23.582930123857373
30	23.628670873862735	25.003037471680045	27.667436159492276	23.700855494964944
31	24.359093475510832	24.963729014644187	26.417427225752043	24.25975028409294
32	23.099793451925756	25.296778850620715	27.7038858196528	23.89954187780073
33	23.175551569122135	24.725019475553715	28.081961706415854	24.017467248908297
34	23.692993803557773	24.568500346629122	27.417291432900466	24.321214416912643
35	23.02617943238588	24.547774069282944	28.03550625719166	24.390540241139515
36	23.400681823054768	24.810783381813764	26.792644315639762	24.995890479491706
37	22.45656415497538	24.434136893488375	27.67243905402411	25.436859897512132
38	22.3257741979288	23.38567313945926	28.91530099557601	25.37325166703593
39	22.642385951872154	23.04690570973206	29.39915236672646	24.911555971669323
40	22.130661311187186	22.551619151080267	30.464768902007588	24.852950635724955
41	21.548896147056514	23.37280855352025	29.77008126130118	25.308214038122056
42	21.24443427983333	22.94041552612583	30.484065780916104	25.33108441312474
43	20.373215932074988	23.428555092589285	30.179603913692922	26.01862506164281
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	8.0
1	15.0
2	22.0
3	79.5
4	137.0
5	137.0
6	189.5
7	242.0
8	238.0
9	234.0
10	348.5
11	463.0
12	463.0
13	941.0
14	1419.0
15	2159.0
16	2899.0
17	2460.0
18	2021.0
19	2021.0
20	2254.0
21	2487.0
22	1748.0
23	1009.0
24	1009.5
25	1010.0
26	1010.0
27	1084.5
28	1159.0
29	1298.5
30	1438.0
31	1609.0
32	1780.0
33	1780.0
34	1994.5
35	2209.0
36	2593.0
37	2977.0
38	3524.0
39	4071.0
40	4071.0
41	4752.0
42	5433.0
43	6488.5
44	7544.0
45	8733.5
46	9923.0
47	9923.0
48	11586.5
49	13250.0
50	13439.5
51	13629.0
52	13107.5
53	12586.0
54	12586.0
55	12514.5
56	12443.0
57	11774.5
58	11106.0
59	9595.5
60	8085.0
61	8085.0
62	7202.5
63	6320.0
64	5384.5
65	4449.0
66	4044.0
67	3639.0
68	3639.0
69	2915.5
70	2192.0
71	1864.5
72	1537.0
73	1229.0
74	921.0
75	921.0
76	770.0
77	619.0
78	473.5
79	328.0
80	256.0
81	184.0
82	184.0
83	137.0
84	90.0
85	61.0
86	32.0
87	23.5
88	15.0
89	15.0
90	11.0
91	7.0
92	4.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	139919.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.34649070743392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21290696696069	64.70107161127915
2	7.557992029023941	11.08704384247328
3	1.7521016970078098	3.8553153251418752
4	0.8180010196186693	2.3999001673652876
5	0.40199961204717477	1.474263040470507
6	0.2859996993699693	1.2586244575301007
7	0.20199978698994642	1.037118284955321
8	0.1239998692251809	0.72759642046782
9	0.09899989559056944	0.6535165429773566
>10	0.4989994737341824	6.502159543763277
>50	0.02999996836077246	1.407517672248361
>100	0.012999986289668065	1.7962536630204462
>500	0.001999997890718164	1.0151143608086146
>1k	0.001999997890718164	2.084505067498615
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1452	1.0377432657466106	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1390	0.9934319141789179	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	869	0.6210736211665321	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	515	0.3680700976993832	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	471	0.33662333207069806	RNA PCR Primer, Index 33 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	314	0.2244155547137987	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	241	0.17224251173893465	No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	221	0.1579485273622596	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	209	0.14937213673625455	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	0.11649597266990189	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	155	0.11077837891923184	No Hit
GTACTTGTTCGCTATCGGTCTCCCGCCAGTATTTAGCCTTAGA	146	0.10434608594972807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0014293984376675076	0.0
16	0.0	0.0	0.0	0.003573496094168769	0.0
17	0.0	0.0	0.0	0.007146992188337538	0.0
18	0.0	0.0	0.0	0.0092910898448388	0.0
19	0.0	0.0	0.0	0.012149886720173815	0.0
20	0.0	0.0	0.0	0.01286458593900757	0.0
21	0.0	0.0	0.0	0.013579285157841323	0.0
22	0.0	0.0	0.0	0.017152781252010092	0.0
23	0.0	0.0	0.0	0.020011578127345105	0.0
24	0.0	0.0	0.0	0.02572917187801514	0.0
25	0.0	0.0	0.0	0.029302667972183907	0.0
26	0.0	0.0	0.0	0.03502026172285394	0.0
27	0.0	0.0	0.0	0.06360823047620409	0.0
28	0.0	0.0	0.0	0.12721646095240818	7.146992188337538E-4
29	0.0	0.0	0.0	0.21155096877479113	7.146992188337538E-4
30	0.0	0.0	0.0	0.40737855473523965	7.146992188337538E-4
31	0.0	0.0	0.0	0.8404862813484945	7.146992188337538E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTT	30	3.5834822E-4	30.833334	7
CGTTATT	25	0.0054800618	29.6	10
ATACTAT	25	0.0054800618	29.6	1
AACATGG	25	0.0054800618	29.6	20
TTAGGAC	40	5.903482E-5	27.75	3
GGACCGT	35	8.830354E-4	26.42857	6
CAGGGTG	40	0.0019227671	23.125	4
ACCGTTA	40	0.0019227671	23.125	8
TAGGACC	40	0.0019227671	23.125	4
GGTATCA	650	0.0	21.915384	1
CTCTGCA	60	3.6962632E-5	21.583334	9
AATGGGA	45	0.0038092865	20.555555	20
GACGCGC	45	0.0038092865	20.555555	6
CCGTTTA	45	0.0038092865	20.555555	27
CCGTTAT	45	0.0038092865	20.555555	9
GCCGTCT	75	9.170757E-6	19.733334	36
TCTGCAA	50	0.0070047937	18.5	10
CCTTATT	50	0.0070047937	18.5	27
TTACTGG	60	9.1816555E-4	18.5	31
ACGGCCG	50	0.0070047937	18.5	20
>>END_MODULE