FastQCFastQC Report
Fri 10 Feb 2017
ERR1632336.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632336.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7887
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT2012.5484975275770254TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT1151.4580956003550145No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC861.090401927222011No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC821.0396855585140106No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG680.8621782680360086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT500.6339546088500064No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC480.6085964244960061Illumina Paired End PCR Primer 2 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG320.4057309496640041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310.39305185748700394No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT260.3296563966020033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.2409027513630024No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG140.1775072904780018No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG130.16482819830100165No Hit
ATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT130.16482819830100165No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA100.12679092177000129No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT80.10143273741600102No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA80.10143273741600102No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCC405.2343625E-527.751
ATCTCCG451.16717696E-424.6666662
GTATGCC502.3858677E-422.234
ATCTCGT502.3858677E-422.229
TATGCCG502.3858677E-422.235
TCTCGTA502.3858677E-422.230
CGTATGC502.3858677E-422.233
CTCGTAT502.3858677E-422.231
GATCTCG554.5457418E-420.18181828
TCGTATG554.5457418E-420.18181832
AGCCCAC608.172153E-418.59
GAGCGAT608.172153E-418.524
GCCCACG608.172153E-418.510
ACTGAGC608.172153E-418.521
TGCCGTC608.172153E-418.537
CCACGAG608.172153E-418.512
ACACTGA608.172153E-418.519
CACTGAG608.172153E-418.520
CCCACGA608.172153E-418.511
GACACTG701.0353765E-418.518