##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632336.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7887
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.71243818942564	34.0	31.0	34.0	31.0	34.0
2	32.84544186636237	34.0	31.0	34.0	31.0	34.0
3	32.88690249778116	34.0	31.0	34.0	31.0	34.0
4	36.35032331685051	37.0	37.0	37.0	35.0	37.0
5	36.226068213515916	37.0	35.0	37.0	35.0	37.0
6	36.25776594395841	37.0	37.0	37.0	35.0	37.0
7	36.20451375681501	37.0	36.0	37.0	35.0	37.0
8	36.20971218460758	37.0	36.0	37.0	35.0	37.0
9	38.02079371117028	39.0	38.0	39.0	35.0	39.0
10	37.94725497654368	39.0	38.0	39.0	35.0	39.0
11	38.05591479650057	39.0	38.0	39.0	35.0	39.0
12	37.94814251299607	39.0	38.0	39.0	35.0	39.0
13	38.03474071256498	39.0	38.0	39.0	35.0	39.0
14	39.31773804995562	40.0	39.0	41.0	36.0	41.0
15	39.298465829846585	40.0	39.0	41.0	37.0	41.0
16	39.28984404716622	40.0	39.0	41.0	36.0	41.0
17	39.30100164828198	40.0	39.0	41.0	36.0	41.0
18	39.35983263598327	40.0	39.0	41.0	37.0	41.0
19	39.33396728794219	40.0	39.0	41.0	36.0	41.0
20	39.3309243058197	40.0	39.0	41.0	36.0	41.0
21	39.37644224673513	40.0	39.0	41.0	36.0	41.0
22	39.27272727272727	40.0	39.0	41.0	36.0	41.0
23	39.28578673766958	40.0	39.0	41.0	36.0	41.0
24	39.188157727906685	40.0	39.0	41.0	36.0	41.0
25	39.13376442246735	40.0	39.0	41.0	36.0	41.0
26	39.02776721186763	40.0	39.0	41.0	35.0	41.0
27	38.83808799289971	40.0	38.0	41.0	35.0	41.0
28	38.87029288702929	40.0	38.0	41.0	35.0	41.0
29	38.7629009762901	40.0	38.0	41.0	35.0	41.0
30	38.6766831494865	40.0	38.0	41.0	35.0	41.0
31	38.54469379992393	40.0	38.0	41.0	35.0	41.0
32	38.329529605680236	40.0	38.0	41.0	34.0	41.0
33	38.17167490807658	40.0	38.0	41.0	34.0	41.0
34	38.02459743882338	40.0	37.0	41.0	33.0	41.0
35	38.0443768226195	40.0	37.0	41.0	34.0	41.0
36	38.02979586661595	40.0	37.0	41.0	33.0	41.0
37	37.92747559274756	40.0	37.0	41.0	33.0	41.0
38	37.81513883605934	40.0	37.0	41.0	33.0	41.0
39	37.75351844807912	40.0	37.0	41.0	33.0	41.0
40	37.56637504754659	40.0	36.0	41.0	33.0	41.0
41	37.43704830734119	40.0	36.0	41.0	33.0	41.0
42	37.31621655889438	40.0	36.0	41.0	33.0	41.0
43	36.33739064282997	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	1.0
23	4.0
24	7.0
25	12.0
26	9.0
27	26.0
28	40.0
29	58.0
30	72.0
31	79.0
32	98.0
33	134.0
34	226.0
35	352.0
36	552.0
37	1036.0
38	2059.0
39	3118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.02624572080639	18.612907315836186	15.100798782807152	27.26004818055027
2	19.208824648155193	19.627234689996197	33.054393305439326	28.10954735640928
3	20.375301128439204	22.04894129580322	32.53455052618233	25.04120704957525
4	14.263978699125143	17.712691771269178	28.058830987701285	39.9644985419044
5	15.405096995055153	33.23190059591733	31.545581336376316	19.817421072651197
6	33.016356028908326	31.976670470394318	15.607962469887157	19.399011030810193
7	25.928743501965258	31.659693165969315	18.410041841004183	24.00152149106124
8	31.355394953721316	28.730822873082285	19.525801952580196	20.387980220616203
9	25.231393432230252	12.501584886522124	18.448079117535183	43.81894256371244
10	20.565487511094204	23.646506910105238	26.01749714720426	29.770508431596298
11	35.01965259287435	20.94586027640421	18.308609103588182	25.72587802713326
12	21.00925573728921	28.426524660834286	24.02687967541524	26.537339926461268
13	34.05604158742234	16.102447064790162	22.619500443768224	27.22201090401927
14	24.686192468619247	21.02193482946621	21.693926714847215	32.59794598706733
15	30.353746671738303	24.610117915557247	18.384683656650182	26.651451756054268
16	29.009762900976288	22.429314061113224	22.25180677063522	26.30911626727526
17	22.25180677063522	29.7958666159503	21.18676302776721	26.765563585647268
18	25.294788893115257	20.742994801572205	23.139343223025232	30.822873082287312
19	25.408900722708257	27.89400278940028	23.278813236972233	23.418283250919234
20	25.12996069481425	20.578166603271207	25.61176619754026	28.680106504374287
21	30.315709395207303	21.03461392164321	23.88740966146824	24.762267021681247
22	28.096868264232285	24.73690883732725	21.96018765056422	25.20603524787625
23	25.561049828832257	24.242424242424242	24.07759604412324	26.11892988462026
24	28.99708380879929	23.088626854317233	23.03791048560923	24.87637885127425
25	25.05388614175225	23.874730569291238	22.91111956383923	28.16026372511728
26	30.936984911880312	21.96018765056422	22.98719411690123	24.115633320654243
27	25.06656523392925	24.470647901610246	23.380245974388234	27.08254089007227
28	25.39622163053125	22.08697857233422	26.321795359452267	26.19500443768226
29	25.64980347407126	23.266134144795235	24.03955876759224	27.04450361354127
30	24.10295422847724	22.226448586281222	27.19665271966527	26.473944465576267
31	25.39622163053125	21.88411309750222	24.724229745150247	27.99543552681628
32	26.46126537339926	23.11398503867123	26.182325345505262	24.242424242424242
33	23.92544693799924	23.266134144795235	27.754532775453278	25.05388614175225
34	25.64980347407126	25.24407252440725	24.686192468619247	24.419931532902243
35	23.253455052618232	21.643210346139217	30.049448459490304	25.05388614175225
36	25.852668948903258	23.088626854317233	23.84937238493724	27.209331811842276
37	22.606821351591226	22.454672245467226	26.765563585647268	28.172942817294285
38	22.79700773424623	21.13604665905921	28.33777101559528	27.729174591099277
39	24.686192468619247	21.14872575123621	26.917712691771268	27.247369088373276
40	21.90947128185622	19.411690122987192	32.77545327754533	25.90338531761126
41	22.150374033219222	21.161404843413212	27.323443641435276	29.364777481932293
42	21.199442119944212	20.1851147457842	32.75009509319133	25.86534804108026
43	20.717636617218208	20.0456447318372	31.266641308482313	27.97007734246228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	4.0
6	8.0
7	12.0
8	8.0
9	4.0
10	7.5
11	11.0
12	11.0
13	28.0
14	45.0
15	58.5
16	72.0
17	69.5
18	67.0
19	67.0
20	73.5
21	80.0
22	57.0
23	34.0
24	32.0
25	30.0
26	30.0
27	36.5
28	43.0
29	50.0
30	57.0
31	76.5
32	96.0
33	96.0
34	102.0
35	108.0
36	125.0
37	142.0
38	155.5
39	169.0
40	169.0
41	221.0
42	273.0
43	352.0
44	431.0
45	482.0
46	533.0
47	533.0
48	631.0
49	729.0
50	719.5
51	710.0
52	768.5
53	827.0
54	827.0
55	678.5
56	530.0
57	689.0
58	848.0
59	709.5
60	571.0
61	571.0
62	459.5
63	348.0
64	316.0
65	284.0
66	254.0
67	224.0
68	224.0
69	218.0
70	212.0
71	192.5
72	173.0
73	132.5
74	92.0
75	92.0
76	70.0
77	48.0
78	47.0
79	46.0
80	32.5
81	19.0
82	19.0
83	13.5
84	8.0
85	7.0
86	6.0
87	3.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	7887.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.1698998351718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.44029732721809	74.10929377456574
2	4.712952712320101	7.556738937492076
3	1.4550055353471452	3.499429440852035
4	0.49027360430175554	1.5722074299480158
5	0.39538193895302864	1.5848865221250157
6	0.12652222046496917	0.6085964244960061
7	0.11070694290684802	0.6212755166730063
8	0.03163055511624229	0.20286547483200204
9	0.0	0.0
>10	0.15815277558121146	3.2458475973120327
>50	0.04744583267436343	2.99226575377203
>100	0.03163055511624229	4.00659312793204
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	201	2.5484975275770254	TruSeq Adapter, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	115	1.4580956003550145	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	86	1.090401927222011	No Hit
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	82	1.0396855585140106	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	68	0.8621782680360086	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	50	0.6339546088500064	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	48	0.6085964244960061	Illumina Paired End PCR Primer 2 (95% over 23bp)
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	32	0.4057309496640041	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31	0.39305185748700394	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.3296563966020033	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.2409027513630024	No Hit
GGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGCAGGTCG	14	0.1775072904780018	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	13	0.16482819830100165	No Hit
ATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCT	13	0.16482819830100165	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	10	0.12679092177000129	No Hit
ACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	8	0.10143273741600102	No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAA	8	0.10143273741600102	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.012679092177000128	0.0
18	0.0	0.0	0.0	0.012679092177000128	0.0
19	0.0	0.0	0.0	0.025358184354000255	0.0
20	0.0	0.0	0.0	0.025358184354000255	0.0
21	0.0	0.0	0.0	0.025358184354000255	0.0
22	0.0	0.0	0.0	0.025358184354000255	0.0
23	0.0	0.0	0.0	0.025358184354000255	0.0
24	0.0	0.0	0.0	0.03803727653100038	0.0
25	0.0	0.0	0.0	0.03803727653100038	0.0
26	0.0	0.0	0.0	0.06339546088500063	0.0
27	0.0	0.0	0.0	0.17750729047800176	0.0
28	0.0	0.0	0.0	0.26626093571700266	0.0
29	0.0	0.0	0.0	0.41841004184100417	0.0
30	0.0	0.0	0.0	0.6593127932040066	0.0
31	0.0	0.0	0.0	1.0396855585140103	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCTCC	40	5.2343625E-5	27.75	1
ATCTCCG	45	1.16717696E-4	24.666666	2
GTATGCC	50	2.3858677E-4	22.2	34
ATCTCGT	50	2.3858677E-4	22.2	29
TATGCCG	50	2.3858677E-4	22.2	35
TCTCGTA	50	2.3858677E-4	22.2	30
CGTATGC	50	2.3858677E-4	22.2	33
CTCGTAT	50	2.3858677E-4	22.2	31
GATCTCG	55	4.5457418E-4	20.181818	28
TCGTATG	55	4.5457418E-4	20.181818	32
AGCCCAC	60	8.172153E-4	18.5	9
GAGCGAT	60	8.172153E-4	18.5	24
GCCCACG	60	8.172153E-4	18.5	10
ACTGAGC	60	8.172153E-4	18.5	21
TGCCGTC	60	8.172153E-4	18.5	37
CCACGAG	60	8.172153E-4	18.5	12
ACACTGA	60	8.172153E-4	18.5	19
CACTGAG	60	8.172153E-4	18.5	20
CCCACGA	60	8.172153E-4	18.5	11
GACACTG	70	1.0353765E-4	18.5	18
>>END_MODULE