FastQCFastQC Report
Fri 10 Feb 2017
ERR1632335.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632335.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9764
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1881.925440393281442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1871.91519868906186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1171.1982793936911103No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT1141.1675542810323638TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.7578861122490782No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT510.5223269151986891No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC480.4916018025399427No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT390.39942646456370345No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC380.38918476034412125RNA PCR Primer, Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC380.38918476034412125No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG270.2765260139287178No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG200.20483408439164277No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA180.18435067595247848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.17410897173289636No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.17410897173289636No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.1638672675133142No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.14338385907414994No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA130.1331421548545678No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGC130.1331421548545678No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA130.1331421548545678No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG120.12290045063498567No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG110.11265874641540352No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA110.11265874641540352No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC110.11265874641540352No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG100.10241704219582139No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT100.10241704219582139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATG450.0035643820.5555551
TGTCTCT608.3733816E-418.537
TATGGGC500.006558593418.54
GGTATCA1106.0489765E-716.8181821
CTGTCTC700.002353905215.85714236
AACGCAG1702.625984E-713.0588245
CAACGCA1702.625984E-713.0588244
GTATCAA1451.0725589E-512.7586212
AGTACAT1204.3639384E-412.33333312
TCAACGC1857.3479714E-712.03
ATCAACG1857.3479714E-712.02
GAGTACA1256.157307E-411.8411
ACATGGG1308.5633865E-411.38461515
CAGAGTA2001.8871433E-611.19
AGAGTAC2001.8871433E-611.110
GCAGAGT2001.8871433E-611.18
CGCAGAG2001.8871433E-611.17
TATCAAC2001.8871433E-611.11
GTACATG1350.001175209510.96296213
TACATGG1350.001175209510.96296214
Produced by FastQC (version 0.11.5)