##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632335.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9764
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14778779188857	33.0	31.0	34.0	30.0	34.0
2	32.24897582957804	33.0	31.0	34.0	30.0	34.0
3	32.23146251536256	34.0	31.0	34.0	30.0	34.0
4	35.902806226956166	37.0	35.0	37.0	35.0	37.0
5	35.71415403523146	37.0	35.0	37.0	33.0	37.0
6	35.787894305612454	37.0	35.0	37.0	35.0	37.0
7	35.71763621466612	37.0	35.0	37.0	35.0	37.0
8	35.74928308070463	37.0	35.0	37.0	35.0	37.0
9	37.39809504301516	39.0	37.0	39.0	35.0	39.0
10	37.28891847603441	39.0	37.0	39.0	34.0	39.0
11	37.42021712412946	39.0	37.0	39.0	35.0	39.0
12	37.336439983613275	39.0	37.0	39.0	34.0	39.0
13	37.45278574354773	39.0	37.0	39.0	35.0	39.0
14	38.536665301106105	40.0	38.0	41.0	35.0	41.0
15	38.48514952888161	40.0	38.0	41.0	34.0	41.0
16	38.41519868906186	40.0	38.0	41.0	34.0	41.0
17	38.46876280213027	40.0	38.0	41.0	34.0	41.0
18	38.60764031134781	40.0	38.0	41.0	35.0	41.0
19	38.64020893076608	40.0	38.0	41.0	35.0	41.0
20	38.655264235968865	40.0	38.0	41.0	35.0	41.0
21	38.61562884063908	40.0	38.0	41.0	35.0	41.0
22	38.505530520278576	40.0	38.0	41.0	34.0	41.0
23	38.41693977877919	40.0	38.0	41.0	34.0	41.0
24	38.29045473166735	40.0	38.0	41.0	34.0	41.0
25	38.24549365014338	40.0	38.0	41.0	34.0	41.0
26	38.07732486685784	40.0	37.0	41.0	34.0	41.0
27	37.89778779188857	40.0	37.0	41.0	33.0	41.0
28	37.79721425645227	40.0	37.0	41.0	33.0	41.0
29	37.64993854977468	39.0	36.0	41.0	33.0	41.0
30	37.46179844326096	39.0	36.0	40.0	33.0	41.0
31	37.18230233510856	39.0	35.0	40.0	33.0	41.0
32	36.90751741089717	39.0	35.0	40.0	32.0	41.0
33	36.6937730438345	38.0	35.0	40.0	31.0	41.0
34	36.476546497337154	38.0	35.0	40.0	31.0	41.0
35	36.37945514133552	38.0	35.0	40.0	31.0	41.0
36	36.21282261368292	38.0	35.0	40.0	31.0	41.0
37	36.04311757476444	38.0	35.0	40.0	30.0	41.0
38	35.921548545678	38.0	35.0	40.0	30.0	41.0
39	35.636112249078245	38.0	35.0	40.0	29.0	41.0
40	35.363068414584184	38.0	35.0	40.0	27.0	41.0
41	35.149631298648096	38.0	35.0	40.0	26.0	41.0
42	34.82640311347808	38.0	34.0	40.0	24.0	41.0
43	33.854260548955345	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	3.0
22	3.0
23	9.0
24	8.0
25	28.0
26	39.0
27	67.0
28	93.0
29	164.0
30	172.0
31	220.0
32	262.0
33	373.0
34	463.0
35	661.0
36	1057.0
37	1876.0
38	2624.0
39	1637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.52355591970504	18.373617369930358	17.01147070872593	22.091356001638673
2	19.029086439983615	22.255223269151987	34.02294141745186	24.692748873412537
3	20.964768537484638	24.62105694387546	32.42523555919705	21.98893895944285
4	14.42031954117165	18.230233510856205	30.52027857435477	36.82916837361737
5	13.426874231872182	37.36173699303564	31.872183531339616	17.339205243752563
6	30.714870954526834	32.507169192953704	17.277755018435066	19.500204834084393
7	24.692748873412537	34.20729209340434	19.73576403113478	21.36419500204834
8	31.544448996312983	30.36665301106104	19.01884473576403	19.07005325686194
9	27.417042195821384	12.67922982384269	19.664072101597707	40.239655878738226
10	20.360507988529292	26.73084801310938	27.611634575993442	25.297009422367882
11	31.667349446947973	23.781237197869725	18.363375665710773	26.18803768947153
12	21.01597705858255	31.718557968045886	25.788611224907825	21.476853748463746
13	34.145841868086855	18.650143383859074	25.09217533797624	22.111839410077838
14	23.17697664891438	23.361327324866856	26.986890618598935	26.47480540761983
15	28.789430561245393	26.218762802130275	23.545678000819336	21.446128635805
16	24.56984842277755	24.928308070462926	27.140516181892664	23.361327324866856
17	19.469479721425646	30.950430151577223	26.77181482998771	22.808275297009423
18	20.45268332650553	22.75706677591151	29.711183941007786	27.079065956575178
19	21.50757886112249	27.847193773043834	30.92994674313806	19.715280622695616
20	24.436706267922983	22.449815649324048	31.923392052437528	21.190086030315445
21	26.444080294961083	23.535436296599755	29.44489963129865	20.575583777140515
22	26.20852109791069	24.65178205653421	28.50266284309709	20.63703400245801
23	22.40884883244572	24.80540761982794	29.946743138058174	22.83900040966817
24	22.02990577632118	26.08562064727571	30.07988529291274	21.80458828349037
25	21.773863170831625	24.590331831216712	30.66366243342892	22.972142564522738
26	23.484227775501843	24.457189676362145	29.219582138467842	22.83900040966817
27	22.60344121261778	25.2867677181483	30.100368701351904	22.009422367882014
28	21.06718557968046	25.2867677181483	31.575174108971733	22.07087259319951
29	20.534616960262188	25.60426054895535	31.442031954117166	22.4190905366653
30	20.45268332650553	26.63867267513314	33.04997951659156	19.858664481769768
31	21.138877509217536	26.966407210159772	30.40761982793937	21.487095452683327
32	20.2990577632118	27.25317492830807	32.384268742318724	20.063498566161407
33	20.73945104465383	26.81278164686604	32.14870954526833	20.2990577632118
34	20.135190495698485	27.76526013928718	30.469070053256864	21.630479311757476
35	19.080294961081524	27.130274477673083	32.957804178615326	20.83162638263007
36	20.524375256042607	27.181482998770996	30.141335518230232	22.152806226956166
37	19.203195411716507	26.741089717328965	30.428103236378533	23.62761163457599
38	20.053256861941826	24.03727980335928	31.790249897582957	24.119213437115935
39	20.063498566161407	25.15362556329373	32.24088488324457	22.541990987300288
40	19.12126177795985	24.32404752150758	33.86931585415813	22.685374846374437
41	17.800081933633756	24.743957394510446	33.5415813191315	23.914379352724293
42	17.605489553461695	24.610815239655878	34.760344121261774	23.023351085620646
43	17.472347398607127	24.692748873412537	34.115116755428105	23.71978697255223
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	10.0
4	18.0
5	18.0
6	24.0
7	30.0
8	35.0
9	40.0
10	60.0
11	80.0
12	80.0
13	145.0
14	210.0
15	299.5
16	389.0
17	340.5
18	292.0
19	292.0
20	331.0
21	370.0
22	250.5
23	131.0
24	122.0
25	113.0
26	113.0
27	109.0
28	105.0
29	126.5
30	148.0
31	154.5
32	161.0
33	161.0
34	169.5
35	178.0
36	181.0
37	184.0
38	238.5
39	293.0
40	293.0
41	326.0
42	359.0
43	433.0
44	507.0
45	600.0
46	693.0
47	693.0
48	842.0
49	991.0
50	930.0
51	869.0
52	792.0
53	715.0
54	715.0
55	668.5
56	622.0
57	584.5
58	547.0
59	554.0
60	561.0
61	561.0
62	484.5
63	408.0
64	332.5
65	257.0
66	212.0
67	167.0
68	167.0
69	140.5
70	114.0
71	105.0
72	96.0
73	76.5
74	57.0
75	57.0
76	40.0
77	23.0
78	20.5
79	18.0
80	13.0
81	8.0
82	8.0
83	7.0
84	6.0
85	3.0
86	0.0
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	9764.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.22613682916837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.19691018591254	72.12208111429742
2	4.7001832940560355	7.353543629659976
3	1.4139827179890023	3.318312167144613
4	0.6022518984027232	1.8844735764031135
5	0.24875621890547264	0.9729619008603032
6	0.15710919088766695	0.7374027038099139
7	0.1178318931657502	0.6452273658336748
8	0.1832940560356114	1.1470708725931995
9	0.03927729772191674	0.2765260139287178
>10	0.2618486514794449	4.055714870954527
>50	0.02618486514794449	1.2802130274477674
>100	0.05236973029588898	6.206472757066775
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	188	1.925440393281442	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	187	1.91519868906186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	117	1.1982793936911103	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	114	1.1675542810323638	TruSeq Adapter, Index 10 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.7578861122490782	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	51	0.5223269151986891	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	48	0.4916018025399427	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.39942646456370345	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	38	0.38918476034412125	RNA PCR Primer, Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	38	0.38918476034412125	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	27	0.2765260139287178	No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG	20	0.20483408439164277	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	18	0.18435067595247848	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.17410897173289636	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.17410897173289636	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16	0.1638672675133142	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.14338385907414994	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	13	0.1331421548545678	No Hit
GTGCTGTGTTTTTAATAAACAGTCGCAGCCACCGATTTTTTGC	13	0.1331421548545678	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	13	0.1331421548545678	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	12	0.12290045063498567	No Hit
ACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATAAGGCG	11	0.11265874641540352	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	11	0.11265874641540352	No Hit
GTTCCGACCTGCACGAATGGCGTAACGATGGCCACACTGTCTC	11	0.11265874641540352	No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG	10	0.10241704219582139	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	10	0.10241704219582139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.010241704219582138	0.0
20	0.0	0.0	0.0	0.010241704219582138	0.0
21	0.0	0.0	0.0	0.020483408439164276	0.0
22	0.0	0.0	0.0	0.030725112658746414	0.0
23	0.0	0.0	0.0	0.04096681687832855	0.0
24	0.0	0.0	0.0	0.04096681687832855	0.0
25	0.0	0.0	0.0	0.04096681687832855	0.0
26	0.0	0.0	0.0	0.04096681687832855	0.0
27	0.0	0.0	0.0	0.07169192953707497	0.0
28	0.0	0.0	0.0	0.14338385907414994	0.0
29	0.0	0.0	0.0	0.297009422367882	0.0
30	0.0	0.0	0.0	0.6247439573945105	0.0
31	0.0	0.0	0.0	1.1265874641540352	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTATG	45	0.00356438	20.555555	1
TGTCTCT	60	8.3733816E-4	18.5	37
TATGGGC	50	0.0065585934	18.5	4
GGTATCA	110	6.0489765E-7	16.818182	1
CTGTCTC	70	0.0023539052	15.857142	36
AACGCAG	170	2.625984E-7	13.058824	5
CAACGCA	170	2.625984E-7	13.058824	4
GTATCAA	145	1.0725589E-5	12.758621	2
AGTACAT	120	4.3639384E-4	12.333333	12
TCAACGC	185	7.3479714E-7	12.0	3
ATCAACG	185	7.3479714E-7	12.0	2
GAGTACA	125	6.157307E-4	11.84	11
ACATGGG	130	8.5633865E-4	11.384615	15
CAGAGTA	200	1.8871433E-6	11.1	9
AGAGTAC	200	1.8871433E-6	11.1	10
GCAGAGT	200	1.8871433E-6	11.1	8
CGCAGAG	200	1.8871433E-6	11.1	7
TATCAAC	200	1.8871433E-6	11.1	1
GTACATG	135	0.0011752095	10.962962	13
TACATGG	135	0.0011752095	10.962962	14
>>END_MODULE