FastQCFastQC Report
Fri 10 Feb 2017
ERR1632334.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632334.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125945
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2730.2167612846877605No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2490.197705347572353No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1670.132597562428044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT251.2273916E-437.021
ATCTAAG352.3715776E-531.71428726
CATCTAA352.3715776E-531.71428725
ACATCTA352.3715776E-531.71428724
GTAGAAC250.005478001729.63
CTAAGGG453.9711085E-628.77777928
CCGGACA358.8254164E-426.42857220
GACATCT358.8254164E-426.42857223
GCGCGTG451.3140915E-424.6666688
AGCGCGC451.3140915E-424.6666685
GGTATCA901.4006218E-1024.6666681
TAAGGGC400.001921702323.12500229
GGACATC400.001921702323.12500222
GCGCGCG502.683628E-422.26
CGCGCGT502.683628E-422.27
TCTAAGG502.683628E-422.227
CGCGTGC502.683628E-422.29
TCTTATA1700.021.76470637
CCCGGAC450.003807197320.55555719
TAGCGCG555.108219E-420.1818184
Produced by FastQC (version 0.11.5)