##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632334.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	125945
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.83855651276351	34.0	31.0	34.0	31.0	34.0
2	33.01283893763151	34.0	33.0	34.0	31.0	34.0
3	33.08781611020684	34.0	33.0	34.0	31.0	34.0
4	36.45357100321569	37.0	37.0	37.0	35.0	37.0
5	36.33207352415737	37.0	37.0	37.0	35.0	37.0
6	36.365921632458615	37.0	37.0	37.0	35.0	37.0
7	36.35165349954345	37.0	37.0	37.0	35.0	37.0
8	36.33678192861964	37.0	37.0	37.0	35.0	37.0
9	38.134352296637424	39.0	39.0	39.0	37.0	39.0
10	38.11926634642106	39.0	38.0	39.0	37.0	39.0
11	38.17421096510382	39.0	39.0	39.0	37.0	39.0
12	38.16014927150741	39.0	39.0	39.0	37.0	39.0
13	38.15991107229346	39.0	39.0	39.0	37.0	39.0
14	39.600817817301206	41.0	39.0	41.0	37.0	41.0
15	39.61063956488944	41.0	39.0	41.0	37.0	41.0
16	39.58986859343364	41.0	39.0	41.0	37.0	41.0
17	39.5933621819048	41.0	39.0	41.0	37.0	41.0
18	39.58390567311128	41.0	39.0	41.0	37.0	41.0
19	39.602461391877405	41.0	39.0	41.0	37.0	41.0
20	39.58751836118941	41.0	39.0	41.0	37.0	41.0
21	39.56214220493072	41.0	39.0	41.0	37.0	41.0
22	39.518766128071775	41.0	39.0	41.0	37.0	41.0
23	39.480392234705626	41.0	39.0	41.0	37.0	41.0
24	39.43338758982095	41.0	39.0	41.0	37.0	41.0
25	39.39053555123268	41.0	39.0	41.0	36.0	41.0
26	39.31088967406408	41.0	39.0	41.0	36.0	41.0
27	39.238072174361825	40.0	39.0	41.0	36.0	41.0
28	39.19209178609711	40.0	39.0	41.0	36.0	41.0
29	39.14240343006868	40.0	39.0	41.0	36.0	41.0
30	39.06879987296042	40.0	39.0	41.0	35.0	41.0
31	39.00617729961491	40.0	39.0	41.0	35.0	41.0
32	38.92760331890905	40.0	38.0	41.0	35.0	41.0
33	38.856135614752475	40.0	38.0	41.0	35.0	41.0
34	38.777664853705986	40.0	38.0	41.0	35.0	41.0
35	38.760855929175435	40.0	38.0	41.0	35.0	41.0
36	38.722886974472985	40.0	38.0	41.0	35.0	41.0
37	38.67643018778038	40.0	38.0	41.0	35.0	41.0
38	38.63805629441423	40.0	38.0	41.0	35.0	41.0
39	38.55381317241653	40.0	38.0	41.0	35.0	41.0
40	38.488221048870535	40.0	38.0	41.0	35.0	41.0
41	38.44470999245703	40.0	38.0	41.0	35.0	41.0
42	38.38834411846441	40.0	38.0	41.0	35.0	41.0
43	37.553797292468936	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	0.0
18	2.0
19	5.0
20	13.0
21	16.0
22	26.0
23	35.0
24	67.0
25	91.0
26	156.0
27	250.0
28	361.0
29	530.0
30	679.0
31	976.0
32	1333.0
33	1863.0
34	2669.0
35	4122.0
36	6637.0
37	12372.0
38	29800.0
39	63940.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.81269601810314	18.831235856921673	12.98900313628965	26.367064988685534
2	19.0908730001191	20.625669935289213	34.65480963912819	25.628647425463498
3	20.455754496010165	21.971495494064868	29.833657548930088	27.73909246099488
4	15.87518361189408	15.956965342014371	33.46619556155465	34.7016554845369
5	16.217396482591607	34.39596649331057	33.4161737266267	15.970463297471118
6	34.26416292826234	33.86160625669935	15.757671999682401	16.11655881535591
7	29.007106276549287	29.408868950732465	21.31485966096312	20.26916511175513
8	26.5473023939021	32.715074040255665	20.5049823335583	20.23264123228393
9	26.164595656834333	14.817579101988965	20.477986422644808	38.5398388185319
10	17.87526301163206	26.702925880344598	32.71031005597681	22.71150105204653
11	35.38131724165311	21.57767279367978	21.500655047838343	21.540354916828775
12	21.58958275437691	25.204652824645677	28.38302433601969	24.82274008495772
13	30.219540275517094	19.532335543292707	24.74016435745762	25.507959823732584
14	23.309381079042442	21.07348445750129	24.520227083250624	31.096907380205646
15	25.47619992854024	27.378617650561754	22.113621025050616	25.031561395847397
16	25.06252729365993	26.05661201318036	23.852475286831552	25.02838540632816
17	23.973956885942275	26.321807138036448	25.481757910198898	24.222478065822383
18	23.6452419707015	25.04823534082338	26.510778514430903	24.795744174044227
19	24.74651633649609	25.283258565246737	26.196355552026677	23.773869546230497
20	24.673468577553695	24.978363571400212	26.08995990313232	24.25820794791377
21	25.027591408948354	25.387272222001666	25.256262654333238	24.328873714716742
22	25.05696931200127	24.754456310294177	25.474611933780615	24.713962443923933
23	24.720314422962407	24.925959744332843	25.528603755607605	24.825122077097145
24	25.00377148755409	25.353130334669892	25.2800825757275	24.363015602048513
25	24.93231172337131	24.57024891817857	25.350748342530473	25.146691015919647
26	24.57183691293819	25.288022549525586	25.546865695343207	24.593274842193022
27	24.80447814522212	24.837826035174086	25.151455000198496	25.206240819405295
28	24.272499900750326	25.137163047361945	25.636587399261586	24.953749652626147
29	23.999364802096153	25.419032117194014	25.989122235896623	24.59248084481321
30	24.04859263964429	26.08440192147366	25.646115367819288	24.220890071062765
31	24.760808289332644	25.095081186232086	25.562745642939376	24.58136488149589
32	24.123228393346302	25.022033427289692	25.940688395728294	24.914049783635715
33	24.139902338322283	24.95216165786653	25.954186351185044	24.953749652626147
34	24.69490650680853	24.555956965342016	25.63738139664139	25.11175513120807
35	24.421771408154353	24.386835523442773	26.617968160705068	24.573424907697806
36	23.899321132240264	24.76398427885188	26.226527452459408	25.110167136448453
37	24.30108380642344	23.303823097383777	26.92842113621025	25.466671959982534
38	23.629362023105323	23.420540712215647	28.0074635753702	24.942633689308824
39	23.1680495454365	23.199809440628847	28.70062328794315	24.931517725991505
40	23.0854738179364	22.936202310532376	28.854658779626025	25.123665091905195
41	22.21207670014689	23.0672118782008	29.42077891142959	25.299932510222717
42	22.3049743935845	23.247449283417364	29.223073563857238	25.224502759140893
43	21.41490333081901	23.347492953273257	29.446980824963276	25.79062289094446
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	14.5
2	14.0
3	26.5
4	39.0
5	39.0
6	60.0
7	81.0
8	85.5
9	90.0
10	115.0
11	140.0
12	140.0
13	251.0
14	362.0
15	518.0
16	674.0
17	670.0
18	666.0
19	666.0
20	849.5
21	1033.0
22	1097.5
23	1162.0
24	1380.5
25	1599.0
26	1599.0
27	1755.5
28	1912.0
29	2334.5
30	2757.0
31	3097.0
32	3437.0
33	3437.0
34	3885.5
35	4334.0
36	4805.5
37	5277.0
38	5744.5
39	6212.0
40	6212.0
41	6814.5
42	7417.0
43	7382.5
44	7348.0
45	7814.5
46	8281.0
47	8281.0
48	8504.5
49	8728.0
50	9192.0
51	9656.0
52	10025.5
53	10395.0
54	10395.0
55	9530.5
56	8666.0
57	8325.5
58	7985.0
59	7320.0
60	6655.0
61	6655.0
62	6157.0
63	5659.0
64	5054.5
65	4450.0
66	3941.0
67	3432.0
68	3432.0
69	2967.5
70	2503.0
71	2168.0
72	1833.0
73	1535.5
74	1238.0
75	1238.0
76	993.5
77	749.0
78	612.0
79	475.0
80	394.0
81	313.0
82	313.0
83	240.0
84	167.0
85	129.0
86	91.0
87	73.0
88	55.0
89	55.0
90	47.5
91	40.0
92	22.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	125945.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.06415498828854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.64291902838234	69.70741196554052
2	4.346600695205587	6.6124101790464085
3	1.2693243144500466	2.896502441541943
4	0.6764162465161432	2.058041208464012
5	0.40919007505297555	1.5562348644249473
6	0.2797523982505037	1.2767477867323038
7	0.2296474911011597	1.2227559649053157
8	0.1701479138613138	1.0353725832704752
9	0.13152538126702784	0.9003930287030053
>10	0.8183801501059511	10.99448171821033
>50	0.02296474911011597	1.1925840644725871
>100	0.003131556696833996	0.5470641946881575
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	273	0.2167612846877605	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	249	0.197705347572353	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	167	0.132597562428044	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	7.939973798086466E-4	0.0
17	0.0	0.0	0.0	0.00238199213942594	0.0
18	0.0	0.0	0.0	0.00238199213942594	0.0
19	0.0	0.0	0.0	0.00238199213942594	0.0
20	0.0	0.0	0.0	0.00238199213942594	0.0
21	0.0	0.0	0.0	0.00238199213942594	0.0
22	0.0	0.0	0.0	0.0031759895192345865	0.0
23	0.0	0.0	0.0	0.0031759895192345865	0.0
24	0.0	0.0	0.0	0.00476398427885188	0.0
25	0.0	0.0	0.0	0.005557981658660526	0.0
26	0.0	0.0	0.0	0.0119099606971297	0.0
27	0.0	0.0	0.0	0.03255389257215451	0.0
28	0.0	0.0	0.0	0.10004366985588947	0.0
29	0.0	7.939973798086466E-4	0.0	0.18500138949541467	0.0
30	0.0	7.939973798086466E-4	0.0	0.2961610226686252	0.0
31	0.0	7.939973798086466E-4	0.0	0.5827940767795466	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGACAT	25	1.2273916E-4	37.0	21
ATCTAAG	35	2.3715776E-5	31.714287	26
CATCTAA	35	2.3715776E-5	31.714287	25
ACATCTA	35	2.3715776E-5	31.714287	24
GTAGAAC	25	0.0054780017	29.6	3
CTAAGGG	45	3.9711085E-6	28.777779	28
CCGGACA	35	8.8254164E-4	26.428572	20
GACATCT	35	8.8254164E-4	26.428572	23
GCGCGTG	45	1.3140915E-4	24.666668	8
AGCGCGC	45	1.3140915E-4	24.666668	5
GGTATCA	90	1.4006218E-10	24.666668	1
TAAGGGC	40	0.0019217023	23.125002	29
GGACATC	40	0.0019217023	23.125002	22
GCGCGCG	50	2.683628E-4	22.2	6
CGCGCGT	50	2.683628E-4	22.2	7
TCTAAGG	50	2.683628E-4	22.2	27
CGCGTGC	50	2.683628E-4	22.2	9
TCTTATA	170	0.0	21.764706	37
CCCGGAC	45	0.0038071973	20.555557	19
TAGCGCG	55	5.108219E-4	20.181818	4
>>END_MODULE