FastQCFastQC Report
Fri 10 Feb 2017
ERR1632333.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632333.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812340
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25500.3139079695693921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT22890.28177856562523085No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21340.26269788512199327No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.10008124676859442No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA200.001841618637.015
GGTATCA8500.029.382351
GTCTAGA1700.020.6764721
CTAGAAC1750.020.0857143
TAGAACA701.2186389E-418.54
TATTATG609.233637E-418.52
GACGGAC801.6157197E-518.57
TTCGACC500.00703314918.532
CTAGACT852.721523E-517.4117644
TAGAACT1950.017.0769234
GTTTTCG650.001579394817.07692328
GTATCAA14700.016.8639452
GTTCTAG2000.016.6500021
TCTAGAC1751.3096724E-1015.8571433
CCGATCG700.002591616515.85714218
GTTTATA700.002591616515.8571421
CTTATAC9100.015.65384737
TCTTATA15750.015.62222237
AAGACGG957.0555994E-515.5789485
AACGGAG1201.9337022E-615.41666625
Produced by FastQC (version 0.11.5)