##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632333.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	812340
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.814357288819956	34.0	31.0	34.0	31.0	34.0
2	32.97345692690253	34.0	33.0	34.0	31.0	34.0
3	33.0602752542039	34.0	33.0	34.0	31.0	34.0
4	36.43352044710343	37.0	37.0	37.0	35.0	37.0
5	36.312025752763624	37.0	37.0	37.0	35.0	37.0
6	36.34301277790088	37.0	37.0	37.0	35.0	37.0
7	36.32234778541005	37.0	37.0	37.0	35.0	37.0
8	36.31304749242928	37.0	37.0	37.0	35.0	37.0
9	38.11577787625871	39.0	39.0	39.0	37.0	39.0
10	38.090981608686015	39.0	38.0	39.0	37.0	39.0
11	38.15331634537263	39.0	39.0	39.0	37.0	39.0
12	38.12434571731048	39.0	38.0	39.0	37.0	39.0
13	38.14209198119014	39.0	39.0	39.0	37.0	39.0
14	39.5330366595268	41.0	39.0	41.0	37.0	41.0
15	39.54382893862176	41.0	39.0	41.0	37.0	41.0
16	39.54268286677992	41.0	39.0	41.0	37.0	41.0
17	39.547646305734055	41.0	39.0	41.0	37.0	41.0
18	39.55417189846616	41.0	39.0	41.0	37.0	41.0
19	39.57904941280744	41.0	39.0	41.0	37.0	41.0
20	39.54970209518182	41.0	39.0	41.0	37.0	41.0
21	39.52735554078342	41.0	39.0	41.0	37.0	41.0
22	39.48902922421646	41.0	39.0	41.0	37.0	41.0
23	39.441455548169486	41.0	39.0	41.0	37.0	41.0
24	39.39362335967698	41.0	39.0	41.0	37.0	41.0
25	39.34327498338134	40.0	39.0	41.0	36.0	41.0
26	39.263639609030704	40.0	39.0	41.0	36.0	41.0
27	39.184713297387795	40.0	39.0	41.0	36.0	41.0
28	39.12025137257798	40.0	39.0	41.0	36.0	41.0
29	39.06443238053032	40.0	39.0	41.0	35.0	41.0
30	38.990198685279566	40.0	39.0	41.0	35.0	41.0
31	38.91438314006451	40.0	38.0	41.0	35.0	41.0
32	38.82815323632961	40.0	38.0	41.0	35.0	41.0
33	38.74766107787379	40.0	38.0	41.0	35.0	41.0
34	38.66176970234138	40.0	38.0	41.0	35.0	41.0
35	38.63561562892385	40.0	38.0	41.0	35.0	41.0
36	38.59281458502598	40.0	38.0	41.0	35.0	41.0
37	38.532920944432135	40.0	38.0	41.0	35.0	41.0
38	38.485056749636854	40.0	38.0	41.0	35.0	41.0
39	38.427754388556515	40.0	38.0	41.0	35.0	41.0
40	38.35504591673437	40.0	38.0	41.0	34.0	41.0
41	38.29293153113228	40.0	38.0	41.0	34.0	41.0
42	38.220294457985574	40.0	38.0	41.0	34.0	41.0
43	37.37015904670458	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	2.0
14	1.0
15	1.0
16	1.0
17	3.0
18	11.0
19	15.0
20	32.0
21	83.0
22	149.0
23	215.0
24	439.0
25	664.0
26	1040.0
27	1602.0
28	2327.0
29	3448.0
30	5055.0
31	6651.0
32	9033.0
33	12552.0
34	18520.0
35	27679.0
36	45406.0
37	86995.0
38	198304.0
39	392109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.33850358224389	17.96082920944432	12.28918925573036	26.41147795258143
2	19.838121968633825	20.153753354506733	33.53448063618682	26.47364404067262
3	20.849890439963563	20.99121057685206	28.717039663195216	29.441859319989167
4	15.594332422384715	14.970086417017505	32.524558682325136	36.91102247827264
5	16.541595883497056	34.36135115838196	32.06871507004456	17.028337888076422
6	36.24061353620405	32.76362114385602	14.968978506536674	16.026786813403255
7	30.666100401309798	28.817859516951028	19.910997858039735	20.605042223699435
8	28.177117955535856	31.713937513848883	19.472511509958885	20.636433020656376
9	27.66871014599798	13.320038407563336	19.263608833739568	39.74764261269911
10	18.305635571312504	25.69576778196322	31.337494152694685	24.661102494029592
11	37.3282123248886	20.638648841618043	19.734101484600046	22.29903734889332
12	23.341334909028237	24.766230888544204	27.587340276239015	24.305093926188544
13	32.113646995100574	18.6275451165768	23.23780682965261	26.021001058670013
14	24.22175443779698	19.805377058866977	24.062215328557006	31.910653174779036
15	26.934904104192825	25.898022995297538	21.29022330551247	25.87684959499717
16	26.29760937538469	24.460078292340643	22.825417928453604	26.41689440382106
17	24.694093606125513	25.05108698328286	24.17066745451412	26.084151956077505
18	25.334219661718	23.168500874018267	25.482187261491497	26.015092202772237
19	25.829209444321343	23.77169658024965	25.164216953492378	25.23487702193663
20	26.424526676022353	23.474284166728218	25.222197601989315	24.878991555260114
21	27.115370411404093	23.351552305684812	24.42856439421917	25.104512888691925
22	26.714183716177953	23.293448556023343	24.412561242829355	25.579806484969346
23	25.75719526306719	23.50998350444395	24.578501612625256	26.154319619863603
24	25.49843661521038	23.892089519166852	24.737794519536155	25.871679346086612
25	26.03909692985696	23.608341334909028	24.50968806164906	25.842873673584954
26	25.951941305364752	23.778220941970112	24.330083462589556	25.939754290075584
27	25.697491198266736	23.478838909816087	24.92527759312603	25.898392298791144
28	25.027574660856295	23.677154885885223	25.091587266415537	26.203683186842948
29	24.84390772336706	23.930250880173325	25.189083388728857	26.036758007730754
30	24.719698648349215	24.314942019351506	25.394293030012065	25.57106630228722
31	25.864785680872544	24.07821847994682	24.41342295098112	25.64357288819952
32	24.66861166506635	23.873131939828152	25.17086441637738	26.28739197872812
33	24.809193194967623	23.44621710121378	25.525518871408526	26.219070832410075
34	25.386291454317156	22.84043627052712	25.602949503902305	26.170322771253417
35	24.902873181180293	23.034197503508384	26.073811458256397	25.98911785705493
36	24.725730605411528	23.07026614471773	25.98025457320826	26.223748676662485
37	24.554373784376	22.391609424625155	26.57581800723835	26.478198783760494
38	24.040180220104883	22.060467292020583	27.47765713863653	26.421695349238007
39	23.87485535613167	21.61127114262501	28.237068222665386	26.276805278577935
40	23.382820001477214	21.961247753403747	28.502474333407196	26.153457911711847
41	22.998990570450797	21.688086269296107	29.282197109584658	26.03072605066844
42	22.613191520791787	21.79604599059507	29.468079868035552	26.122682620577592
43	21.524731023955486	21.740527365388875	29.62257182952951	27.112169781126127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	60.0
2	81.0
3	227.0
4	373.0
5	373.0
6	485.5
7	598.0
8	589.0
9	580.0
10	850.0
11	1120.0
12	1120.0
13	1901.5
14	2683.0
15	3878.0
16	5073.0
17	4934.0
18	4795.0
19	4795.0
20	5169.5
21	5544.0
22	4889.0
23	4234.0
24	4853.5
25	5473.0
26	5473.0
27	6402.0
28	7331.0
29	8848.5
30	10366.0
31	12238.0
32	14110.0
33	14110.0
34	16865.0
35	19620.0
36	22923.5
37	26227.0
38	29140.0
39	32053.0
40	32053.0
41	34865.5
42	37678.0
43	41048.5
44	44419.0
45	48887.0
46	53355.0
47	53355.0
48	55431.0
49	57507.0
50	60323.0
51	63139.0
52	64865.5
53	66592.0
54	66592.0
55	66743.5
56	66895.0
57	64611.5
58	62328.0
59	60594.0
60	58860.0
61	58860.0
62	53774.5
63	48689.0
64	42771.5
65	36854.0
66	32120.5
67	27387.0
68	27387.0
69	22924.5
70	18462.0
71	15624.0
72	12786.0
73	9763.0
74	6740.0
75	6740.0
76	5249.0
77	3758.0
78	3066.0
79	2374.0
80	2064.0
81	1754.0
82	1754.0
83	1384.5
84	1015.0
85	858.0
86	701.0
87	574.0
88	447.0
89	447.0
90	339.5
91	232.0
92	140.5
93	49.0
94	29.0
95	9.0
96	9.0
97	8.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	812340.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.032564944971
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49010251929722	58.197709040183085
2	5.389445081274818	7.0097887453071825
3	1.6874948540833188	3.2922635607743342
4	0.8550483632610177	2.2242395285945316
5	0.5130483123429285	1.6682423846174632
6	0.3327333619450493	1.2983102382029976
7	0.2591748519861991	1.1798363775727019
8	0.16904719589603473	0.879485819669929
9	0.14493744448906448	0.8483088394523892
>10	1.004115233053224	12.781701061433484
>50	0.10860641642146762	4.867986281137434
>100	0.045488228804902844	4.792433488722884
>500	1.8953428617343555E-4	0.10020957329971478
>1k	5.686028585203067E-4	0.859485061031871
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2550	0.3139079695693921	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2289	0.28177856562523085	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2134	0.26269788512199327	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.10008124676859442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.2310116453701652E-4	0.0
4	0.0	0.0	0.0	1.2310116453701652E-4	0.0
5	0.0	0.0	0.0	1.2310116453701652E-4	0.0
6	0.0	0.0	0.0	1.2310116453701652E-4	0.0
7	0.0	0.0	0.0	1.2310116453701652E-4	0.0
8	0.0	0.0	0.0	1.2310116453701652E-4	0.0
9	0.0	0.0	0.0	2.4620232907403305E-4	0.0
10	0.0	0.0	0.0	2.4620232907403305E-4	1.2310116453701652E-4
11	0.0	0.0	0.0	8.617081517591157E-4	1.2310116453701652E-4
12	0.0	0.0	0.0	0.0013541128099071817	1.2310116453701652E-4
13	0.0	0.0	0.0	0.0014772139744441983	1.2310116453701652E-4
14	0.0	0.0	0.0	0.0022158209616662972	1.2310116453701652E-4
15	0.0	0.0	0.0	0.003569933771573479	1.2310116453701652E-4
16	0.0	0.0	0.0	0.006893665214072925	1.2310116453701652E-4
17	0.0	0.0	0.0	0.010340497821109388	1.2310116453701652E-4
18	0.0	0.0	0.0	0.01095600364379447	1.2310116453701652E-4
19	0.0	0.0	0.0	0.012187015289164636	1.2310116453701652E-4
20	0.0	0.0	0.0	0.01304872344092375	1.2310116453701652E-4
21	0.0	0.0	0.0	0.015264544402590048	1.2310116453701652E-4
22	0.0	0.0	0.0	0.020680995642218775	1.2310116453701652E-4
23	0.0	0.0	0.0	0.025974345717310485	1.2310116453701652E-4
24	0.0	0.0	0.0	0.035822438880271805	1.2310116453701652E-4
25	0.0	0.0	0.0	0.04074648546175247	1.2310116453701652E-4
26	0.0	0.0	0.0	0.046901543688603296	1.2310116453701652E-4
27	0.0	0.0	0.0	0.0707831696087845	1.2310116453701652E-4
28	0.0	0.0	0.0	0.15227614053228944	1.2310116453701652E-4
29	0.0	0.0	0.0	0.29470418790161756	1.2310116453701652E-4
30	0.0	0.0	0.0	0.4783711253908462	1.2310116453701652E-4
31	0.0	0.0	0.0	0.9438166285053057	1.2310116453701652E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATTA	20	0.0018416186	37.0	15
GGTATCA	850	0.0	29.38235	1
GTCTAGA	170	0.0	20.676472	1
CTAGAAC	175	0.0	20.085714	3
TAGAACA	70	1.2186389E-4	18.5	4
TATTATG	60	9.233637E-4	18.5	2
GACGGAC	80	1.6157197E-5	18.5	7
TTCGACC	50	0.007033149	18.5	32
CTAGACT	85	2.721523E-5	17.411764	4
TAGAACT	195	0.0	17.076923	4
GTTTTCG	65	0.0015793948	17.076923	28
GTATCAA	1470	0.0	16.863945	2
GTTCTAG	200	0.0	16.650002	1
TCTAGAC	175	1.3096724E-10	15.857143	3
CCGATCG	70	0.0025916165	15.857142	18
GTTTATA	70	0.0025916165	15.857142	1
CTTATAC	910	0.0	15.653847	37
TCTTATA	1575	0.0	15.622222	37
AAGACGG	95	7.0555994E-5	15.578948	5
AACGGAG	120	1.9337022E-6	15.416666	25
>>END_MODULE