FastQCFastQC Report
Fri 10 Feb 2017
ERR1632332.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632332.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457354
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAAGT405.9324317E-527.752
AGCGTAT358.861198E-426.428578
CGTGTCG358.861198E-426.4285727
GTGTCGT451.3215106E-424.66666828
TGTCGTG451.3215106E-424.66666829
GGTATCA1850.024.01
CAACGTA400.001929419623.12517
AGTACGA400.001929419623.12537
CGTAGCC450.003822338820.5555573
TACTAAC450.003822338820.5555577
ACGGTAT450.003822338820.5555579
ACAACGT500.00702856518.516
GACCGTG500.00702856518.57
TTATAGA500.00702856518.54
TCTAATA500.00702856518.52
GTACTAT609.225227E-418.51
CGTGTAG500.00702856518.532
ACTAACA650.001577968717.0769238
TATACAC1655.4569682E-1116.81818236
CTTATAC4100.016.69512237
Produced by FastQC (version 0.11.5)