FastQCFastQC Report
Fri 10 Feb 2017
ERR1632331.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632331.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences963119
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.17318732160823325No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT15090.15667845821752038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14280.14826828252791194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12540.13020197919467896No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC11690.12137648618706515No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC10560.10964377195341386No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG250.005495907729.5999984
GGTATCA6700.026.7835831
TCAATCG450.00382536420.55555713
CTTATAC8900.020.1629237
CTAGACA2700.019.1851844
TCTAGAC2550.018.8627453
GTAGACC902.1514788E-618.54
CTCGTAT500.00703407618.49999829
GTATCAA10300.017.4223292
CTCTAGT2550.017.41176427
GTTCTAG2800.017.1785721
GAACGGT650.001579682617.07692320
GTCTAGA2100.016.7380941
TCTTATA16150.016.72445937
TAGCGCC904.4447515E-516.44444529
AAGACGG2550.015.9607845
TATACAC2900.015.94827637
ATTCCGC700.002592084515.85714328
CCTCTAG4100.015.79268226
CGCAAGA2600.015.6538462
Produced by FastQC (version 0.11.5)